Abstract
Rhizosphere bacteria play a vital role in plant growth, pathogen control, biodegradation and rhizosphere signaling. A motile, rod-shaped bacterium, Pv2Z2, isolated from the nodules of the common bean grown in Tanzanian soil was characterized using a polyphasic approach. The traits assessed included the production of indole-3-acetic acid and N-acyl homoserine lactone (AHL) molecules, solubilization of insoluble phosphate and zinc compounds and biodegradation of a number of toxic compounds. The 16S rRNA sequence of Pv2Z2 (EU399793) showed 99 % homology to Ochrobactrum anthropi isolates (Accession no. AJ867292, AJ867291, AJ867290) from soil samples of wheat root. Phylogenetic analysis showed relatedness to nodulating strain Ochrobactrum cytisi rather than to the clinical/pathogenic type strain of O. anthropi. Moreover, it showed unique fingerprints in the randomly amplified polymorphic DNA (RAPD) and two primers-RAPD assays which were different from those of the pathogenic type strain of O. anthropi. The bacterium produced 6.68 μg/mL-1 indoleacetic acid in the presence of tryptophan, released 25.7 μg/mL-1 phosphorus from inorganic tri-calcium phosphate in the Pikoviskaya’s medium and solubilized zinc sulphate and zinc oxide in the LG1 medium. The production of AHLs (e.g. 3O-C7-HSL, 3OH-C7-HSL) was detected with biosensor strains CV026 using reverse phase thin layer chromatography. The bacterium was able to grow in minimal salt medium supplemented with 100 mg/L each of phenol, 2-bromophenol, 2,4-diamino phenol hydrochloride, 3,4-dimethoxy benzyl alcohol and 4-methoxy benzyl alcohol. Phenol degradation was recorded up to a level of 94 % within 12 days. Inoculation of common bean plants resulted in a significant increase in plant height, fresh/dry weight and nitrogen uptake as compared to non-inoculated plants. The data suggest that the plant growth-promoting and biodegradation potential of this bacterium may be exploited on a large scale. The capacity to produce AHL molecules by members of the Ochrobactrum genus has not been previously reported and needs to be explored in detail.
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Claims 14
Claim · model-written summary
Pv2Z2 showed 99 % 16S rRNA sequence homology to Ochrobactrum anthropi isolates.
- Taxon
- common bean
Phaseolus vulgarise1 - Interactor
- Pv2Z2e0symbiont
- Outcome
- homology to Ochrobactrum anthropi isolatese0
- Value
- 99 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 16S rRNA sequence of Pv2Z2 (EU399793) showed 99 % homology to Ochrobactrum anthropi isolates”
e1“nodules of the common bean”
Claim · model-written summary
Phylogenetic analysis placed Pv2Z2 closer to nodulating Ochrobactrum cytisi than to the pathogenic O. anthropi type strain.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- relatedness to nodulating strain Ochrobactrum cytisie0
- Comparator
- clinical/pathogenic type strain of O. anthropie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis showed relatedness to nodulating strain Ochrobactrum cytisi rather than to the clinical/pathogenic type strain of O. anthropi”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
Pv2Z2 had RAPD fingerprints different from the pathogenic O. anthropi type strain.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- unique fingerprints in the randomly amplified polymorphic DNA (RAPD) and two primers-RAPD assayse0
- Comparator
- pathogenic type strain of O. anthropie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it showed unique fingerprints in the randomly amplified polymorphic DNA (RAPD) and two primers-RAPD assays which were different from those of the pathogenic type strain of O. anthropi”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
The bean nodule bacterium produced 6.68 μg/mL-1 indoleacetic acid in the presence of tryptophan.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- indoleacetic acide0
- Value
- 6.68 μg/mL-1e0
- Qualifier
- in the presence of tryptophane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bacterium produced 6.68 μg/mL-1 indoleacetic acid in the presence of tryptophan”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
The bean nodule bacterium released 25.7 μg/mL-1 phosphorus from tri-calcium phosphate.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- phosphorus from inorganic tri-calcium phosphatee0
- Value
- 25.7 μg/mL-1e0
- Setting
- Pikoviskaya’s mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“released 25.7 μg/mL-1 phosphorus from inorganic tri-calcium phosphate in the Pikoviskaya’s medium”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
The bean nodule bacterium solubilized zinc sulphate and zinc oxide.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- solubilized zinc sulphate and zinc oxidee0
- Setting
- LG1 mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“solubilized zinc sulphate and zinc oxide in the LG1 medium”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
AHL molecules including 3O-C7-HSL and 3OH-C7-HSL were detected from the bean nodule bacterium.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- production of AHLs (e.g. 3O-C7-HSL, 3OH-C7-HSL)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The production of AHLs (e.g. 3O-C7-HSL, 3OH-C7-HSL) was detected with biosensor strains CV026 using reverse phase thin layer chromatography”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
The bean nodule bacterium grew in minimal salt medium supplemented with five listed aromatic compounds at 100 mg/L each.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- able to growe0
- Value
- 100 mg/Le0
- Setting
- minimal salt mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The bacterium was able to grow in minimal salt medium supplemented with 100 mg/L each of phenol, 2-bromophenol, 2,4-diamino phenol hydrochloride, 3,4-dimethoxy benzyl alcohol and 4-methoxy benzyl alcohol”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
The bean nodule bacterium degraded phenol by up to 94 % within 12 days.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- Phenol degradatione0
- Value
- 94 %e0
- Qualifier
- within 12 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phenol degradation was recorded up to a level of 94 % within 12 days”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
Inoculated common bean plants were significantly taller than non-inoculated plants.
- Taxon
- common beanPhaseolus vulgarise0
- Treatment
- Inoculatione0
- Interactor
- Pv2Z2e1symbiont
- Outcome
- plant heighte0
- Direction
- increasee0
- Comparator
- non-inoculated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of common bean plants resulted in a significant increase in plant height, fresh/dry weight and nitrogen uptake as compared to non-inoculated plants”
e1“bacterium, Pv2Z2”
Claim · model-written summary
Inoculated common bean plants had significantly greater fresh and dry weight than non-inoculated plants.
- Taxon
- common beanPhaseolus vulgarise0
- Treatment
- Inoculatione0
- Interactor
- Pv2Z2e1symbiont
- Outcome
- fresh/dry weighte0
- Direction
- increasee0
- Comparator
- non-inoculated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of common bean plants resulted in a significant increase in plant height, fresh/dry weight and nitrogen uptake as compared to non-inoculated plants”
e1“bacterium, Pv2Z2”
Claim · model-written summary
Inoculated common bean plants had significantly greater nitrogen uptake than non-inoculated plants.
- Taxon
- common beanPhaseolus vulgarise0
- Treatment
- Inoculatione0
- Interactor
- Pv2Z2e1symbiont
- Outcome
- nitrogen uptakee0
- Direction
- increasee0
- Comparator
- non-inoculated plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of common bean plants resulted in a significant increase in plant height, fresh/dry weight and nitrogen uptake as compared to non-inoculated plants”
e1“bacterium, Pv2Z2”
Claim · model-written summary
The authors suggest exploiting the bacterium’s plant-growth-promoting and biodegradation potential at large scale.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Pv2Z2e2symbiont
- Outcome
- may be exploited on a large scalee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the plant growth-promoting and biodegradation potential of this bacterium may be exploited on a large scale”
e1“nodules of the common bean”
e2“bacterium, Pv2Z2”
Claim · model-written summary
The authors state that AHL production by Ochrobactrum members had not previously been reported and needs further study.
- Taxon
- common beanPhaseolus vulgarise1
- Interactor
- Ochrobactrum genuse0symbiont
- Outcome
- needs to be explored in detaile0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The capacity to produce AHL molecules by members of the Ochrobactrum genus has not been previously reported and needs to be explored in detail”
e1“nodules of the common bean”
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See all 44 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.