Abstract
Watermelon chlorotic stunt virus (WmCSV) is a bipartite begomovirus (genus Begomovirus, family Geminiviridae) that causes economic losses to cucurbits, particularly watermelon, across the Middle East and North Africa. Recently squash (Cucurbita moschata) grown in an experimental field in Oman was found to display symptoms such as leaf curling, yellowing and stunting, typical of a begomovirus infection. Sequence analysis of the virus isolated from squash showed 97.6-99.9% nucleotide sequence identity to previously described WmCSV isolates for the DNA A component and 93-98% identity for the DNA B component. Agrobacterium-mediated inoculation to Nicotiana benthamiana resulted in the development of symptoms fifteen days post inoculation. This is the first bipartite begomovirus identified in Oman. Overall the Oman isolate showed the highest levels of sequence identity to a WmCSV isolate originating from Iran, which was confirmed by phylogenetic analysis. This suggests that WmCSV present in Oman has been introduced from Iran. The significance of this finding is discussed.
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Claims 6
Claim · model-written summary
Squash in Oman displayed leaf curling, yellowing and stunting.
- Taxon
- squash (Cucurbita moschata)
Cucurbita moschatae0 - Outcome
- leaf curling, yellowing and stuntinge0
- Setting
- Omane0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“squash (Cucurbita moschata) grown in an experimental field in Oman was found to display symptoms such as leaf curling, yellowing and stunting”
Claim · model-written summary
The squash virus DNA A had 97.6-99.9% identity to prior WmCSV isolates.
Verbatim
e0“virus isolated from squash showed 97.6-99.9% nucleotide sequence identity to previously described WmCSV isolates for the DNA A component”
Claim · model-written summary
The squash virus DNA B had 93-98% identity to prior WmCSV isolates.
Verbatim
e0“virus isolated from squash showed 97.6-99.9% nucleotide sequence identity to previously described WmCSV isolates for the DNA A component and 93-98% identity for the DNA B component”
Claim · model-written summary
Inoculated Nicotiana benthamiana developed symptoms after fifteen days.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Agrobacterium-mediated inoculatione0
- Outcome
- development of symptomse0
- Qualifier
- fifteen days post inoculatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Agrobacterium-mediated inoculation to Nicotiana benthamiana resulted in the development of symptoms fifteen days post inoculation.”
Claim · model-written summary
The Oman isolate had highest sequence identity to an Iran isolate, supported by phylogeny.
- Interactor
- WmCSVe0pathogen
- Outcome
- sequence identitye0
- Direction
- higheste0
- Comparator
- WmCSV isolate originating from Irane0
Not reportedTaxon
Verbatim
e0“the Oman isolate showed the highest levels of sequence identity to a WmCSV isolate originating from Iran, which was confirmed by phylogenetic analysis”
Claim · model-written summary
Authors suggest WmCSV in Oman was introduced from Iran.
- Interactor
- WmCSVe0pathogen
- Outcome
- WmCSV present in Oman has been introduced from Irane0
- Qualifier
- Omane0
Not reportedTaxon
Verbatim
e0“This suggests that WmCSV present in Oman has been introduced from Iran.”
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See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.