Paper · 2013 · Communications in Soil Science and Plant Analysis

Ascophyllum nodosumExtract and Its Organic Fractions StimulateRhizobiumRoot Nodulation and Growth ofMedicago sativa(Alfalfa)

Wajahatullah Khan; Ravishankar Palanisamy; Alan T. Critchley; et al.

13
claims
23
verbatim quotes
30
citations
3
research areas
—
Not open access

Abstract

The effects of the seaweed Ascophyllum nodosum extracts (ANE) on nitrogen (N)–fixing nodules and growth of alfalfa plants were studied under greenhouse conditions. The treatment of alfalfa roots increased the number of total nodules per plant with ANE (69%) and organic sub-fractions methanol (20%) and chloroform (35%) at 1 g L−1 concentration. The number of functional nodules was greater per plant in ANE (36%) and its organic sub-fraction chloroform (105%). Maximum increase in shoot length was observed in ANE-treated plants (42%) and chloroform-treated plants (42%). Root length was longer in the chloroform fraction (15%), whereas the shoot dry-weight accumulation was greater in plants treated with ANE (118%), methanol (85%), and chloroform (85%) than the control. Root dry-weight accumulation increased in plants treated with ANE (118%) and chloroform (69%) compared to the control. Further studies are under way to identify the chemical components in ANE and organic fractions.

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Claims 13

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ANE increased total nodules per alfalfa plant by 69%.

Taxon
alfalfaMedicago sativae0
Treatment
ANEe0
Outcome
number of total nodules per plante0
Direction
increasede0
Value
69%e0
Qualifier
1 g L−1 concentratione0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The treatment of alfalfa roots increased the number of total nodules per plant with ANE (69%) and organic sub-fractions methanol (20%) and chloroform (35%) at 1 g L−1 concentration”

✓ located verbatim in the source · abstract, characters 164–344 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Methanol subfraction increased total nodules per alfalfa plant by 20%.

Taxon
alfalfaMedicago sativae0
Treatment
methanole0
Outcome
number of total nodules per plante0
Direction
increasede0
Value
20%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The treatment of alfalfa roots increased the number of total nodules per plant with ANE (69%) and organic sub-fractions methanol (20%) and chloroform (35%) at 1 g L−1 concentration”

✓ located verbatim in the source · abstract, characters 164–344 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chloroform subfraction increased total nodules per alfalfa plant by 35%.

Taxon
alfalfaMedicago sativae0
Treatment
chloroforme0
Outcome
number of total nodules per plante0
Direction
increasede0
Value
35%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The treatment of alfalfa roots increased the number of total nodules per plant with ANE (69%) and organic sub-fractions methanol (20%) and chloroform (35%) at 1 g L−1 concentration”

✓ located verbatim in the source · abstract, characters 164–344 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ANE increased functional nodules per alfalfa plant by 36%.

Taxon
alfalfaMedicago sativae1
Treatment
ANEe0
Outcome
number of functional nodulese0
Direction
greatere0
Value
36%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The number of functional nodules was greater per plant in ANE (36%) and its organic sub-fraction chloroform (105%)”

✓ located verbatim in the source · abstract, characters 346–460 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chloroform subfraction increased functional nodules per alfalfa plant by 105%.

Taxon
alfalfaMedicago sativae1
Treatment
chloroforme0
Outcome
number of functional nodulese0
Direction
greatere0
Value
105%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“The number of functional nodules was greater per plant in ANE (36%) and its organic sub-fraction chloroform (105%)”

✓ located verbatim in the source · abstract, characters 346–460 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ANE increased alfalfa shoot length by 42%.

Taxon
alfalfaMedicago sativae1
Treatment
ANE-treatede0
Outcome
shoot lengthe0
Direction
increasee0
Value
42%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Maximum increase in shoot length was observed in ANE-treated plants (42%) and chloroform-treated plants (42%)”

✓ located verbatim in the source · abstract, characters 462–571 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chloroform fraction increased alfalfa shoot length by 42%.

Taxon
alfalfaMedicago sativae1
Treatment
chloroform-treatede0
Outcome
shoot lengthe0
Direction
increasee0
Value
42%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Maximum increase in shoot length was observed in ANE-treated plants (42%) and chloroform-treated plants (42%)”

✓ located verbatim in the source · abstract, characters 462–571 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chloroform fraction increased alfalfa root length by 15%.

Taxon
alfalfaMedicago sativae1
Treatment
chloroform fractione0
Outcome
Root lengthe0
Direction
longere0
Value
15%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Root length was longer in the chloroform fraction (15%)”

✓ located verbatim in the source · abstract, characters 573–628 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ANE increased alfalfa shoot dry weight by 118% relative to control.

Taxon
alfalfaMedicago sativae1
Treatment
ANEe0
Outcome
shoot dry-weight accumulatione0
Direction
greatere0
Value
118%e0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“shoot dry-weight accumulation was greater in plants treated with ANE (118%), methanol (85%), and chloroform (85%) than the control”

✓ located verbatim in the source · abstract, characters 642–772 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Methanol fraction increased alfalfa shoot dry weight by 85% relative to control.

Taxon
alfalfaMedicago sativae1
Treatment
methanole0
Outcome
shoot dry-weight accumulatione0
Direction
greatere0
Value
85%e0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“shoot dry-weight accumulation was greater in plants treated with ANE (118%), methanol (85%), and chloroform (85%) than the control”

✓ located verbatim in the source · abstract, characters 642–772 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chloroform fraction increased alfalfa shoot dry weight by 85% relative to control.

Taxon
alfalfaMedicago sativae1
Treatment
chloroforme0
Outcome
shoot dry-weight accumulatione0
Direction
greatere0
Value
85%e0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“shoot dry-weight accumulation was greater in plants treated with ANE (118%), methanol (85%), and chloroform (85%) than the control”

✓ located verbatim in the source · abstract, characters 642–772 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ANE increased alfalfa root dry weight by 118% relative to control.

Taxon
alfalfaMedicago sativae1
Treatment
ANEe0
Outcome
Root dry-weight accumulatione0
Direction
increasede0
Value
118%e0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Root dry-weight accumulation increased in plants treated with ANE (118%) and chloroform (69%) compared to the control”

✓ located verbatim in the source · abstract, characters 774–891 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Chloroform fraction increased alfalfa root dry weight by 69% relative to control.

Taxon
alfalfaMedicago sativae1
Treatment
chloroforme0
Outcome
Root dry-weight accumulatione0
Direction
increasede0
Value
69%e0
Comparator
the controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Root dry-weight accumulation increased in plants treated with ANE (118%) and chloroform (69%) compared to the control”

✓ located verbatim in the source · abstract, characters 774–891 · show in text ↑
e1

“growth of alfalfa plants”

✓ located verbatim in the source · abstract, characters 97–121 · show in text ↑

methanol · growth · Medicago sativa (alfalfa)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 in the opposite direction

  1. negative
See all 2 papers →

botanical · growth · Medicago sativa (alfalfa)Results differ

101 papers: 42 positive, 59 negative or no effect

41 papers in the same direction as this paper · 59 in the opposite direction

  1. negative
  2. negative
  3. negative

97 papers more in this group

See all 101 papers →

botanical · symbiosis · Medicago sativa (alfalfa)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.