Abstract
*When supplied with arsenate (As(V)), plant roots extrude a substantial amount of arsenite (As(III)) to the external medium through as yet unidentified pathways. The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots. Whether Lsi1 also mediates arsenite efflux was investigated. *Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux, indicating that Lsi1 facilitates arsenite transport bidirectionally. *Arsenite was the predominant arsenic species in arsenate-exposed rice plants. During 24-h exposure to 5 mum arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake. A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots. By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium. *We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux, suggesting the existence of other efflux transporters.
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Claims 6
Claim · model-written summary
Expression of Lsi1 in Xenopus oocytes enhanced arsenite efflux, so Lsi1 transports arsenite bidirectionally.
- Taxon
- rice
Oryza sativae1 - Treatment
- Expression of Lsi1 in Xenopus laevis oocytese2
- Gene
- Lsi1e2
- Outcome
- arsenite effluxe2
- Direction
- enhancede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots”
e2“Expression of Lsi1 in Xenopus laevis oocytes enhanced arsenite efflux”
Claim · model-written summary
During 24 h exposure to 5 mum arsenate, rice roots extruded arsenite, accounting for 60-90% of arsenate uptake.
- Taxon
- riceOryza sativae1
- Organ / stage
- rice rootse3
- Treatment
- 5 mum arsenatee3
- Outcome
- extruded arsenitee3
- Value
- 60-90%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots”
e3“During 24-h exposure to 5 mum arsenate, rice roots extruded arsenite to the external medium rapidly, accounting for 60-90% of the arsenate uptake”
Claim · model-written summary
The Lsi1 mutant extruded significantly less arsenite than wild-type rice.
- Taxon
- riceOryza sativae4
- Material
- lsi1e4
- Treatment
- defective in Lsi1e4
- Gene
- Lsi1e4
- Outcome
- extruded significantly less arsenitee4
- Direction
- lesse4
- Comparator
- wild-type ricee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots”
e4“A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots”
Claim · model-written summary
The Lsi1 mutant accumulated more arsenite in the roots than wild-type rice.
- Taxon
- riceOryza sativae4
- Treatment
- defective in Lsi1e4
- Gene
- Lsi1e4
- Outcome
- arsenite in the rootse4
- Direction
- accumulated moree4
- Comparator
- wild-type ricee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots”
e4“A rice mutant defective in Lsi1 (lsi1) extruded significantly less arsenite than the wild-type rice and, as a result, accumulated more arsenite in the roots”
Claim · model-written summary
Lsi2 mutation had little effect on arsenite efflux from rice roots.
- Taxon
- riceOryza sativae1
- Treatment
- Lsi2 mutatione5
- Gene
- Lsi2e5
- Outcome
- arsenite effluxe5
- Direction
- had little effecte5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots”
e5“By contrast, Lsi2 mutation had little effect on arsenite efflux to the external medium”
Claim · model-written summary
The Lsi1 pathway accounts for only 15-20% of total arsenite efflux, suggesting other efflux transporters.
- Taxon
- riceOryza sativae6
- Gene
- Lsi1e6
- Outcome
- total effluxe6
- Value
- 15-20%e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The rice (Oryza sativa) silicon transporter Lsi1 (OsNIP2;1, an aquaporin channel) is the major entry route of arsenite into rice roots”
e6“We conclude that Lsi1 plays a role in arsenite efflux in rice roots exposed to arsenate. However, this pathway accounts for only 15-20% of the total efflux”
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