Abstract
In vitro cultural conditions were investigated for promoting differentiation and growth of bulblets from segments of various parts of plantlets regenerated through shoot-tip culture of lilies (Lilium spp.). The growth and flowering of the in vitro propagated bulblets were observed in soil culture, and diagnosis of virus was also performed under electron microscope and by sap inoculation to L. formosanum seedlings.1. Bulblet differentiation from various tissue segments and the growth of bulblets were enhanced with addition of auxin, especially 0.1mg/l NAA, to the medium. Addition of cytokinin to the medium multiplied the differentiation of bulblet primordia (adventitious buds) remarkably, but retarded their development to bulblets.2. Cutting bulb scales into segments increased total number of regenerated bulblets per scale.3. High sucrose concentration of the medium (60-120g/l) and high incubation temperature (27-28°C) inhibited leaf elongation from bulblets and promoted enlargement of the base of scaly leaves.4. Although both leaf and stem-node segments regenerated bulblets, bulb scales were most suitable material for in vitro propagation of lilies because of their high regeneration rate of bulblets and simplicity of their culture process.5. In L. longiflorum, in vitro propagated bulblets flowered without cold treatment within one year after transplanting to soil, and bulbs with marketable size for cut-flower production were harvested in the first cultivation in soil. On the other hand, cold treatment to the bulblets of L. speciosum accelerated bolting in soil culture and reduced days to flowering. In the case of L.×formolongo and L. concolor, bulblets propagated by in vitro culture could flower earlier than by conventional cultivation methods in field conditions. Lilies like these two species are considered to be promising materials for practical production of bulblets through in vitro culture.6. Diagnosis of virus in bulblets derived from shoot-tip culture of L. longiflorum indicated that these bulblets were freed from virus.These results suggested that a large number of virus-free lily bulbs can be propagated in a short time by subculturing bulb scales of in vitro bulblets derived from shoot-tip culture, and that the cultivation time necessary to flowering-bulb production can be reduced remarkably by use of those bulbs.
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Claims 18
Claim · model-written summary
Auxin, especially 0.1mg/l NAA, enhanced bulblet differentiation and growth.
- Treatment
- addition of auxin, especially 0.1mg/l NAAe0
- Outcome
- Bulblet differentiation from various tissue segments and the growth of bulbletse0
- Direction
- enhancede0
LiliumNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Bulblet differentiation from various tissue segments and the growth of bulblets were enhanced with addition of auxin, especially 0.1mg/l NAA, to the medium.”
t0“Lilies”
Claim · model-written summary
Cytokinin increased bulblet primordia differentiation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of cytokinin to the medium multiplied the differentiation of bulblet primordia (adventitious buds) remarkably”
t0“Lilies”
Claim · model-written summary
Cytokinin retarded primordia development into bulblets.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but retarded their development to bulblets.”
t0“Lilies”
Claim · model-written summary
Cutting bulb scales into segments increased regenerated bulblets per scale.
- Treatment
- Cutting bulb scales into segmentse0
- Outcome
- total number of regenerated bulblets per scalee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cutting bulb scales into segments increased total number of regenerated bulblets per scale.”
t0“Lilies”
Claim · model-written summary
High sucrose concentration inhibited bulblet leaf elongation.
- Treatment
- High sucrose concentration of the medium (60-120g/l)e0
- Outcome
- leaf elongation from bulbletse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High sucrose concentration of the medium (60-120g/l) and high incubation temperature (27-28°C) inhibited leaf elongation from bulblets”
t0“Lilies”
Claim · model-written summary
High incubation temperature inhibited bulblet leaf elongation.
- Treatment
- high incubation temperature (27-28°C)e0
- Outcome
- leaf elongation from bulbletse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high incubation temperature (27-28°C) inhibited leaf elongation from bulblets”
t0“Lilies”
Claim · model-written summary
High sucrose concentration promoted enlargement of scaly leaf bases.
- Treatment
- High sucrose concentration of the medium (60-120g/l)e0
- Outcome
- enlargement of the base of scaly leavese0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High sucrose concentration of the medium (60-120g/l) and high incubation temperature (27-28°C) inhibited leaf elongation from bulblets and promoted enlargement of the base of scaly leaves.”
t0“Lilies”
Claim · model-written summary
High incubation temperature promoted enlargement of scaly leaf bases.
- Treatment
- high incubation temperature (27-28°C)e0
- Outcome
- enlargement of the base of scaly leavese0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high incubation temperature (27-28°C) inhibited leaf elongation from bulblets and promoted enlargement of the base of scaly leaves.”
t0“Lilies”
Claim · model-written summary
Bulb scales were the most suitable propagation material, though leaf and stem-node segments also regenerated bulblets.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although both leaf and stem-node segments regenerated bulblets, bulb scales were most suitable material for in vitro propagation of lilies because of their high regeneration rate of bulblets and simplicity of their culture process.”
Claim · model-written summary
L. longiflorum bulblets flowered without cold treatment within one year.
- Taxon
- L. longiflorum
.JPG?width=60)
Lilium longiflorume0 - Outcome
- flowered without cold treatmente0
- Value
- within one yeare0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.JPG?width=500)
Verbatim
e0“In L. longiflorum, in vitro propagated bulblets flowered without cold treatment within one year after transplanting to soil”
Claim · model-written summary
L. longiflorum bulbs reached marketable cut-flower size in their first soil cultivation.
- Outcome
- marketable size for cut-flower productione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bulbs with marketable size for cut-flower production were harvested in the first cultivation in soil.”
t0“Lilies”
Claim · model-written summary
Cold treatment accelerated bolting in L. speciosum.
- Taxon
- L. speciosum
Lilium speciosume0 - Treatment
- cold treatmente0
- Outcome
- boltinge0
- Direction
- acceleratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold treatment to the bulblets of L. speciosum accelerated bolting in soil culture”
Claim · model-written summary
Cold treatment reduced days to flowering in L. speciosum.
- Taxon
- L. speciosumLilium speciosume0
- Treatment
- cold treatmente0
- Outcome
- days to floweringe0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“cold treatment to the bulblets of L. speciosum accelerated bolting in soil culture and reduced days to flowering.”
Claim · model-written summary
In vitro propagated L.×formolongo flowered earlier than conventional field cultivation.
Verbatim
e0“In the case of L.×formolongo and L. concolor, bulblets propagated by in vitro culture could flower earlier than by conventional cultivation methods in field conditions.”
Claim · model-written summary
In vitro propagated L. concolor flowered earlier than conventional field cultivation.
- Taxon
- L. concolor
Lilium concolore0 - Treatment
- in vitro culturee0
- Outcome
- flowere0
- Direction
- earliere0
- Comparator
- conventional cultivation methodse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the case of L.×formolongo and L. concolor, bulblets propagated by in vitro culture could flower earlier than by conventional cultivation methods in field conditions.”
Claim · model-written summary
Shoot-tip derived L. longiflorum bulblets were virus-free.
- Taxon
- L. longiflorumLilium longiflorume0
- Outcome
- freed from viruse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Diagnosis of virus in bulblets derived from shoot-tip culture of L. longiflorum indicated that these bulblets were freed from virus.”
Claim · model-written summary
Subculturing scales of shoot-tip derived bulblets could produce many virus-free bulbs quickly.
- Outcome
- virus-free lily bulbs can be propagated in a short timee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a large number of virus-free lily bulbs can be propagated in a short time by subculturing bulb scales of in vitro bulblets derived from shoot-tip culture”
Claim · model-written summary
Use of those bulbs could substantially shorten production of flowering bulbs.
- Outcome
- cultivation time necessary to flowering-bulb production can be reduced remarkablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cultivation time necessary to flowering-bulb production can be reduced remarkably by use of those bulbs.”
t0“Lilies”
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