Abstract
Leaves and pith of Turkish, Wisconsin 38, and Samsun NN tobacco (Nicotiana tabacum) varieties, which differ in their sensitivity to tobacco mosaic virus, showed the same qualitative isoperoxidase patterns and a similar distribution of distinctive isoperoxidases between the cell protoplast and wall-free, ionically, and covalently bound fractions. No changes in the qualitative isoenzyme spectrum were found in relation to age, mechanical injury, or leaf infection with tobacco mosaic virus. The distinctive isoperoxidases which reacted to infection were the same as those responsive to mechanical injury, confirming that the enzyme reaction to infection results from a nonspecific response to injury. The increase in peroxidase activity in response to infection or mechanical injury, or both, was greater in young tissue than in the older ones. The great increase in Samsun NN leaves and no increase in those of the two other varieties in response to infection may be due to differences in the degree to which the pathogen affected processes controlling the nonspecific peroxidase reaction to injury. Peroxidase development in the infected Samsun NN leaves was due to isoenzymes which form the wall-bound fraction in very young tissues, and to those which increase in activity with aging in the protoplast and wall-free fractions. In mechanically injured tissue, only the first group of isoenzymes increased in activity. In Samsun NN plants, the increased peroxidase activity in upper intact leaves above the infected ones was only due to isoenzymes whose activity increases with both normal and virus-accelerated senescence. Peroxidase reaction to challenge inoculation in these leaves was the same whether the lower ones were intact, infected and/or mechanically injured. Thus, the induced systemic resistance to tobacco mosaic virus may be due to other than peroxidase factors.In infected tissues, peroxidase was detected in the endoplasmic reticulum, Golgi apparatus, vacuole, cell wall, and intercellular spaces. The Golgi vesicles were often localized near the tonoplast and plasmalemma, fusing with membranes and secreting their contents. The possible "rejuvenating" effects of injury on synthesis and transport of distinctive isoperoxidases are discussed.
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Claims 12
Claim · model-written summary
Three tobacco varieties shared qualitative isoperoxidase patterns and fraction distributions.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Gene
- isoperoxidasese0
- Outcome
- same qualitative isoperoxidase patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Turkish, Wisconsin 38, and Samsun NN tobacco (Nicotiana tabacum) varieties, which differ in their sensitivity to tobacco mosaic virus, showed the same qualitative isoperoxidase patterns and a similar distribution of distinctive isoperoxidases between the cell protoplast and wall-free, ionically, and covalently bound fractions”
Claim · model-written summary
Age, injury, and virus infection caused no qualitative isoenzyme-spectrum changes.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Outcome
- qualitative isoenzyme spectrume0
- Direction
- No changese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No changes in the qualitative isoenzyme spectrum were found in relation to age, mechanical injury, or leaf infection with tobacco mosaic virus”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Infection-responsive isoperoxidases were the same as injury-responsive isoperoxidases.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- mechanical injurye0
- Gene
- isoperoxidasese0
- Outcome
- distinctive isoperoxidasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The distinctive isoperoxidases which reacted to infection were the same as those responsive to mechanical injury”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Peroxidase activity increased more in young than older tissue after infection or injury.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Organ / stage
- young tissuee0
- Treatment
- infection or mechanical injurye0
- Gene
- peroxidasee0
- Outcome
- peroxidase activitye0
- Direction
- greatere0
- Comparator
- older onese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in peroxidase activity in response to infection or mechanical injury, or both, was greater in young tissue than in the older ones”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Infected Samsun NN leaves showed a large peroxidase increase.
- Taxon
- TobaccoNicotiana tabacumt0
- Material
- Samsun NNe0
- Gene
- Isoperoxidasest0
- Outcome
- increasee0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The great increase in Samsun NN leaves and no increase in those of the two other varieties in response to infection”
t0“Cell Wall and Protoplast Isoperoxidases in Tobacco Plants in Relation to Mechanical Injury and Infection with Tobacco Mosaic Virus”
Claim · model-written summary
The other two varieties showed no peroxidase increase after infection.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Outcome
- no increasee0
- Direction
- no increasee0
- Qualifier
- two other varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no increase in those of the two other varieties in response to infection”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Infection in Samsun NN activated wall-bound and age-associated isoenzymes.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Material
- Samsun NNe0
- Gene
- isoenzymese0
- Outcome
- Peroxidase developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Peroxidase development in the infected Samsun NN leaves was due to isoenzymes which form the wall-bound fraction in very young tissues, and to those which increase in activity with aging in the protoplast and wall-free fractions”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Mechanical injury increased only the first isoenzyme group.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- mechanically injurede0
- Outcome
- isoenzymes increased in activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In mechanically injured tissue, only the first group of isoenzymes increased in activity”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Peroxidase increases in upper intact Samsun NN leaves involved senescence-associated isoenzymes.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Material
- Samsun NNe0
- Organ / stage
- upper intact leavese0
- Outcome
- peroxidase activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Samsun NN plants, the increased peroxidase activity in upper intact leaves above the infected ones was only due to isoenzymes whose activity increases with both normal and virus-accelerated senescence”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Challenge-inoculation response in upper leaves was the same regardless of lower-leaf condition.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Treatment
- challenge inoculatione0
- Outcome
- Peroxidase reactione0
- Direction
- the samee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Peroxidase reaction to challenge inoculation in these leaves was the same whether the lower ones were intact, infected and/or mechanically injured”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
In infected tissues peroxidase was detected in five cellular locations.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Organ / stage
- infected tissuese0
- Gene
- peroxidasee0
- Outcome
- peroxidase was detectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In infected tissues, peroxidase was detected in the endoplasmic reticulum, Golgi apparatus, vacuole, cell wall, and intercellular spaces”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Golgi vesicles were observed fusing with membranes and secreting contents.
- Taxon
- Nicotiana tabacumNicotiana tabacumt0
- Outcome
- Golgi vesicles were often localized near the tonoplast and plasmalemmae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Golgi vesicles were often localized near the tonoplast and plasmalemma, fusing with membranes and secreting their contents”
t0“tobacco (Nicotiana tabacum)”
The same questions in other papers
infection · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Positive and negative results
3 papers in the same direction as this paper · 4 in the opposite direction
- no effect
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.