Abstract
The Arabidopsis thaliana AtHMA3 protein belongs to the P(1B)-adenosine triphosphatase (ATPase) transporter family, involved in heavy metal transport. Functional expression of AtHMA3 phenotypically complements the Cd/Pb-hypersensitive yeast strain Deltaycf1, but not the Zn-hypersensitive mutant Deltazrc1. AtHMA3-complemented Deltaycf1 cells accumulate the same amount of cadmium as YCF1-complemented Deltaycf1 cells or wild-type cells, suggesting that AtHMA3 carries out an intracellular sequestration of Cd. A mutant of AtHMA3 altered in the P-ATPase phosphorylation domain did not complement Deltaycf1, suggesting that metal transport rather than chelation is involved. The fusion protein AtHMA3::green fluorescent protein (GFP) is localized at the vacuole, consistent with a role in the influx of cadmium into the vacuolar compartment. In A. thaliana, the mRNA of AtHMA3 was detected mainly in roots, old rosette leaves and cauline leaves. The expression levels were not affected by cadmium or zinc treatments.
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Claims 7
Claim · model-written summary
AtHMA3 complemented cadmium/lead hypersensitivity in yeast Deltaycf1.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- Functional expression of AtHMA3e0
- Gene
- AtHMA3e0
- Outcome
- phenotypically complementse0
- Comparator
- Cd/Pb-hypersensitive yeast strain Deltaycf1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional expression of AtHMA3 phenotypically complements the Cd/Pb-hypersensitive yeast strain Deltaycf1, but not the Zn-hypersensitive mutant Deltazrc1.”
t0“The Arabidopsis thaliana AtHMA3 protein”
Claim · model-written summary
AtHMA3 did not complement zinc-hypersensitive yeast Deltazrc1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Functional expression of AtHMA3e0
- Gene
- AtHMA3e0
- Outcome
- not the Zn-hypersensitive mutant Deltazrc1e0
- Comparator
- Zn-hypersensitive mutant Deltazrc1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional expression of AtHMA3 phenotypically complements the Cd/Pb-hypersensitive yeast strain Deltaycf1, but not the Zn-hypersensitive mutant Deltazrc1.”
t0“The Arabidopsis thaliana AtHMA3 protein”
Claim · model-written summary
AtHMA3-complemented yeast accumulated the same cadmium as YCF1-complemented or wild-type cells.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtHMA3e0
- Outcome
- amount of cadmiume0
- Direction
- samee0
- Comparator
- YCF1-complemented Deltaycf1 cells or wild-type cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“AtHMA3-complemented Deltaycf1 cells accumulate the same amount of cadmium as YCF1-complemented Deltaycf1 cells or wild-type cells”
t0“The Arabidopsis thaliana AtHMA3 protein”
Claim · model-written summary
A phosphorylation-domain AtHMA3 mutant failed to complement Deltaycf1.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- A mutant of AtHMA3e0
- Treatment
- altered in the P-ATPase phosphorylation domaine0
- Gene
- AtHMA3e0
- Outcome
- did not complemente0
- Comparator
- Deltaycf1e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A mutant of AtHMA3 altered in the P-ATPase phosphorylation domain did not complement Deltaycf1”
t0“The Arabidopsis thaliana AtHMA3 protein”
Claim · model-written summary
AtHMA3::GFP localized at the vacuole.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- AtHMA3::green fluorescent protein (GFP)e0
- Outcome
- localized at the vacuolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fusion protein AtHMA3::green fluorescent protein (GFP) is localized at the vacuole”
t0“The Arabidopsis thaliana AtHMA3 protein”
Claim · model-written summary
AtHMA3 mRNA was detected mainly in roots, old rosette leaves and cauline leaves.
- Taxon
- A. thalianaArabidopsis thalianae0
- Organ / stage
- roots, old rosette leaves and cauline leavese0
- Gene
- AtHMA3e0
- Outcome
- mRNA of AtHMA3 was detected mainlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In A. thaliana, the mRNA of AtHMA3 was detected mainly in roots, old rosette leaves and cauline leaves.”
Claim · model-written summary
Cadmium and zinc treatments did not affect AtHMA3 expression levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cadmium or zinc treatmentse0
- Gene
- AtHMA3t1
- Outcome
- expression levelse0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression levels were not affected by cadmium or zinc treatments.”
t0“The Arabidopsis thaliana AtHMA3 protein”
t1“AtHMA3”
The same questions in other papers
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- positive
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See all 6 papers →cadmium atom · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- positive
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.