Abstract
Three novel bacterial strains, designated Vu-144(T), Vu-7 and Vu-35, were isolated on minimal medium from rhizosphere soil of field-grown cowpea and subjected to a taxonomic study using a polyphasic approach. Cells of the strains were Gram-stain-negative, non-motile, non-spore-forming, coccoid rods, and formed non-pigmented colonies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Vu-144(T) was affiliated with an uncultivated lineage of the phylum Bacteroidetes. Its closest phylogenetic neighbour was the recently described species Niastella populi, a member of the family Chitinophagaceae, with just 90.7 % sequence similarity to the type strain. The only isoprenoid quinone detected was menaquinone 7 (MK-7). The fatty acid profiles showed large amounts of iso-C15 : 0, iso-C17 : 0 3-OH and iso-C15 : 1 G and minor amounts of summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH), C16 : 0 and other fatty acids, allowing the differentiation of the strains from other genera. The G+C content of the genomic DNA of the three strains ranged from 43.1 to 44.3 mol%. In addition to phosphatidylethanolamine, the major polar lipids were three unidentified aminophospholipids (APL1-APL3), two unidentified phospholipids (PL1, PL2) and three unidentified lipids (UL1-UL3). Biochemical test patterns also differed from those of Niastella populi and members of other genera. All three isolates showed plant-growth-promoting properties, e.g. the ability to produce indole-3-acetic acid and NH3 and to solubilize phosphate, utilized 1-aminocyclopropane 1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions. Inoculated plants showed a 45-60 % increase in dry matter weight with respect to uninoculated controls. On the basis of the evidence from our polyphasic study, isolate Vu-144(T) represents a novel genus and species in the family Chitinophagaceae, for which the name Arachidicoccus rhizosphaerae gen. nov., sp. nov. is proposed. The type strain of Arachidicoccus rhizosphaerae is Vu-144(T) ( = KCTC 22378(T) = NCIMB 14473(T)).
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Claims 4
Claim · model-written summary
The novel bacterial isolates colonized tomato roots.
- Taxon
- tomato
Solanum lycopersicume0 - Interactor
- novel isolatese0symbiont
- Outcome
- colonized rootse0
- Setting
- glasshouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions”
Claim · model-written summary
The novel bacterial isolates colonized cowpea roots.
- Taxon
- cowpea
Vigna unguiculatae0 - Interactor
- novel isolatese0symbiont
- Outcome
- colonized rootse0
- Setting
- glasshouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions”
Claim · model-written summary
Inoculation stimulated tomato growth and raised dry matter weight by 45–60% versus controls.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Inoculatede0
- Interactor
- novel isolatese0symbiont
- Outcome
- dry matter weighte0
- Direction
- increasee0
- Value
- 45-60 %e0
- Comparator
- uninoculated controlse0
- Setting
- glasshouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions. Inoculated plants showed a 45-60 % increase in dry matter weight with respect to uninoculated controls”
Claim · model-written summary
Inoculation stimulated cowpea growth and raised dry matter weight by 45–60% versus controls.
- Taxon
- cowpeaVigna unguiculatae0
- Treatment
- Inoculatede0
- Interactor
- novel isolatese0symbiont
- Outcome
- dry matter weighte0
- Direction
- increasee0
- Value
- 45-60 %e0
- Comparator
- uninoculated controlse0
- Setting
- glasshouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions. Inoculated plants showed a 45-60 % increase in dry matter weight with respect to uninoculated controls”
The same questions in other papers
inoculation · growth · Vigna unguiculata (cowpea)Positive and negative results
91 papers in the same direction as this paper · 8 in the opposite direction · 2 reporting no difference from an alternative
- no effect
- negative
- no effect
98 papers more in this group
See all 102 papers →inoculation · growth · Solanum lycopersicum (tomato)Positive and negative results
386 papers in the same direction as this paper · 53 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- no effect
- no effect
437 papers more in this group
See all 441 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.