Abstract
The exocyst is a protein complex that is essential for polarized secretion in mammals and fungi. Although the exocyst is essential for plant development, its precise function has not been elucidated. We studied the role of exocyst subunit SEC3A in plant development and its subcellular localization. T-DNA insertional mutants were identified and complemented with a SEC3A-green fluorescent protein (GFP) fusion construct. SEC3A-GFP localization was determined using confocal microscopy. sec3a mutants are defective in the globular to heart stage transition in embryogenesis. SEC3A-GFP has similar cell plate localization to the other plant exocyst subunits. In interphase cells, SEC3A-GFP localizes to the cytoplasm and to the plasma membrane, where it forms immobile, punctate structures with discrete lifetimes of 2-40 s. These puncta are equally distributed over the cell surface of root epidermal cells and tip growing root hairs. The density of puncta does not decrease after growth termination of these cells, but decreases strongly when exocytosis is inhibited by treatment with brefeldin A. SEC3A does not appear to be involved in polarized secretion for cell expansion in tip growing root hairs. The landmark function performed by SEC3 in mammals and yeast is likely to be conserved in plants.
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Claims 8
Claim · model-written summary
sec3a mutants were defective in the globular to heart stage transition of embryogenesis.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- sec3a mutantse0
- Outcome
- globular to heart stage transition in embryogenesise0
- Direction
- defectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“sec3a mutants are defective in the globular to heart stage transition in embryogenesis.”
Claim · model-written summary
SEC3A-GFP localized to the cell plate similarly to other plant exocyst subunits.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- cell platee0
- Gene
- SEC3A-GFPe0
- Outcome
- cell plate localizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“SEC3A-GFP has similar cell plate localization to the other plant exocyst subunits.”
Claim · model-written summary
In interphase cells SEC3A-GFP localized to the cytoplasm and plasma membrane as immobile puncta lasting 2–40 seconds.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- interphase cellse0
- Gene
- SEC3A-GFPe0
- Outcome
- immobile, punctate structurese0
- Value
- 2-40 se0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“In interphase cells, SEC3A-GFP localizes to the cytoplasm and to the plasma membrane, where it forms immobile, punctate structures with discrete lifetimes of 2-40 s.”
Claim · model-written summary
SEC3A puncta were equally distributed on root epidermal cells and growing root hairs.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- root epidermal cells and tip growing root hairse0
- Outcome
- punctae0
- Direction
- equally distributede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“These puncta are equally distributed over the cell surface of root epidermal cells and tip growing root hairs.”
Claim · model-written summary
SEC3A puncta density did not decrease after these cells stopped growing.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- density of punctae0
- Direction
- does not decreasee0
- Qualifier
- after growth terminatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“The density of puncta does not decrease after growth termination of these cells, but decreases strongly when exocytosis is inhibited by treatment with brefeldin A.”
Claim · model-written summary
Brefeldin A inhibition of exocytosis strongly decreased SEC3A puncta density.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- treatment with brefeldin Ae0
- Outcome
- density of punctae0
- Direction
- decreases stronglye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“The density of puncta does not decrease after growth termination of these cells, but decreases strongly when exocytosis is inhibited by treatment with brefeldin A.”
Claim · model-written summary
SEC3A did not appear involved in polarized secretion for expansion of tip growing root hairs.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- tip growing root hairse0
- Gene
- SEC3Ae0
- Outcome
- polarized secretion for cell expansione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“SEC3A does not appear to be involved in polarized secretion for cell expansion in tip growing root hairs.”
Claim · model-written summary
The authors suggested SEC3 landmark function is conserved in plants.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- SEC3e0
- Outcome
- landmark functione0
- Qualifier
- likely to be conserved in plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“The landmark function performed by SEC3 in mammals and yeast is likely to be conserved in plants.”