Abstract
Since previous investigations had suggested that kinetin, like auxin, may initiate DNA synthesis in tobacco pith cells, a study was undertaken to learn whether the observed incorporation of tritiated thymidine into nuclear DNA in the presence of kinetin could be explained by different experimental factors.Pith tissues were isolated and allowed to rest a few days after excision and they were then placed on White's basic medium or on that medium supplemented with either one or both of the growth regulators in the presence of tritiated thymidine.The results of those studies, obtained by radioautographic and liquid scintillation counting methods, showed no statistically significant differences between pith tissues kept on the control medium and those on kinetin-containing medium. Similar tissues placed on an auxin or auxin- and kinetin-containing medium showed, as expected, a significant incorporation of tritiated thymidine into the DNA of pith cell nuclei.
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Claims 2
Claim · model-written summary
Kinetin-containing medium did not significantly change incorporation of tritiated thymidine into nuclear DNA of tobacco pith tissue compared with control medium
- Taxon
- tobacco
Nicotiana tabacumt0 - Treatment
- kinetin-containing mediume1
- Outcome
- incorporation of tritiated thymidine into nuclear DNAe0
- Direction
- no_changeinferrede1
- Comparator
- control mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Initiation of DNA synthesis by kinetin and experimental factors in tobacco pith tissues in vitro”
e0“the observed incorporation of tritiated thymidine into nuclear DNA in the presence of kinetin”
e1“showed no statistically significant differences between pith tissues kept on the control medium and those on kinetin-containing medium”
Claim · model-written summary
Auxin and auxin-plus-kinetin media significantly increased incorporation of tritiated thymidine into the DNA of tobacco pith cell nuclei
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- auxin or auxin- and kinetin-containing mediume0
- Outcome
- incorporation of tritiated thymidine into the DNA of pith cell nucleie0
- Direction
- increaseinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Initiation of DNA synthesis by kinetin and experimental factors in tobacco pith tissues in vitro”
e0“Similar tissues placed on an auxin or auxin- and kinetin-containing medium showed, as expected, a significant incorporation of tritiated thymidine into the DNA of pith cell nuclei”
The same questions in other papers
in vitro · molecular: metabolites · Nicotiana tabacum (tobacco)Positive and negative results
5 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.