Abstract
Transgenic rice containing an antisense cDNA for the alpha subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds. In normal rice, the mRNA for the alpha subunit was abundant in the internodes and florets, the tissues closely related to abnormality in the dwarf transformants. The position of the alpha-subunit gene was mapped on rice chromosome 5 by mapping with the restriction fragment length polymorphism. The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d-1), which is known to exhibit abnormal phenotypes similar to those of the transformants that suppressed the endogenous mRNA for the alpha subunit by antisense technology. Analysis of the cDNAs for the alpha subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the alpha-subunit gene. These results show that the G protein functions in the formation of normal internodes and seeds in rice.
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Claims 7
Claim · model-written summary
Antisense rice produced little or no alpha subunit mRNA.
- Taxon
- rice
Oryza sativae0 - Treatment
- antisense cDNAe0
- Gene
- alpha subunit of rice heterotrimeric G proteine0
- Outcome
- mRNA for the subunite0
- Direction
- little or noe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice containing an antisense cDNA for the alpha subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit”
Claim · model-written summary
Antisense rice exhibited dwarf traits and small seeds.
- Taxon
- riceOryza sativae0
- Treatment
- antisense cDNAe0
- Outcome
- dwarf traits and the setting of small seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice containing an antisense cDNA for the alpha subunit of rice heterotrimeric G protein produced little or no mRNA for the subunit and exhibited abnormal morphology, including dwarf traits and the setting of small seeds.”
Claim · model-written summary
Normal rice had abundant alpha subunit mRNA in internodes and florets.
- Taxon
- riceOryza sativae0
- Organ / stage
- internodes and floretse0
- Gene
- alpha subunite0
- Outcome
- mRNA for the alpha subunit was abundante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In normal rice, the mRNA for the alpha subunit was abundant in the internodes and florets”
Claim · model-written summary
The alpha subunit gene mapped to rice chromosome 5.
- Taxon
- riceOryza sativae0
- Gene
- alpha-subunit genee0
- Outcome
- mapped on rice chromosome 5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The position of the alpha-subunit gene was mapped on rice chromosome 5”
Claim · model-written summary
The alpha subunit gene was closely linked to the Daikoku dwarf locus.
- Taxon
- riceOryza sativae0
- Gene
- Daikoku dwarf (d-1)e0
- Outcome
- closely linked to the locuse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The position was closely linked to the locus of a rice dwarf mutant, Daikoku dwarf (d-1)”
Claim · model-written summary
Five Daikoku dwarf alleles had coding region mutations in the alpha subunit gene.
- Taxon
- riceOryza sativat0
- Material
- Daikoku dwarf (d-1)e0
- Gene
- alpha-subunit genee0
- Outcome
- mutated in the coding region of the alpha-subunit genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the cDNAs for the alpha subunits of five alleles of Daikoku dwarf (d-1), ID-1, DK22, DKT-1, DKT-2, and CM1361-1, showed that these dwarf mutants had mutated in the coding region of the alpha-subunit gene.”
t0“rice”
Claim · model-written summary
The G protein functions in formation of normal rice internodes and seeds.
- Taxon
- riceOryza sativae0
- Gene
- G proteine0
- Outcome
- formation of normal internodes and seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the G protein functions in the formation of normal internodes and seeds in rice”
The same questions in other papers
loss of function · molecular: expression · Oryza sativa (rice)Positive and negative results
66 papers in the same direction as this paper · 31 in the opposite direction
- negative
- positive
- positive
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See all 98 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.