Abstract
Abscisic acid and osmoticum maintain maturation and protein synthesis of developing alfalfa embryos, individually and in combination. The in situ environment of developing alfalfa zygotic embryos is rich in ABA and low in osmotic potential. When ABA synthesis was inhibited by treating the pods with fluridone at an early stage of development, the seeds which subsequently developed contained low amounts of ABA, but had a similar osmotic potential as untreated control seeds. The reduced ABA in seeds from fluridone-treated pods did not change the morphology except the colour of seeds, nor did it induce viviparous germination or affect storage protein synthesis. However, two nonstorage proteins which were synthesized in control seeds during early to mid-development were absent from fluridone-treated seeds. Control seeds containing these two proteins were desiccation-tolerant, whereas the fluridone-treated seeds which lacked them were desiccation-intolerant, at least until the deposition of storage proteins was nearly complete. Culture of isolated embryos on nutrient medium induced germination and curtailed storage protein synthesis in the embryos. Addition of either ABA or osmoticum to the nutrient medium prevented germination and maintained storage protein synthesis. When fluridone was added along with osmoticum, germination occurred, but storage protein synthesis was maintained.
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Claims 15
Claim · model-written summary
ABA and osmoticum each maintain maturation and protein synthesis in developing alfalfa embryos.
- Taxon
- alfalfa
Medicago sativae0 - Organ / stage
- developing alfalfa embryose0
- Treatment
- Abscisic acid and osmoticume0
- Outcome
- maturation and protein synthesise0
- Direction
- maintaine0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid and osmoticum maintain maturation and protein synthesis of developing alfalfa embryos, individually and in combination.”
Claim · model-written summary
Developing alfalfa zygotic embryos have high ABA and low osmotic potential in situ.
- Taxon
- alfalfaMedicago sativae0
- Organ / stage
- developing alfalfa zygotic embryose0
- Outcome
- rich in ABA and low in osmotic potentiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in situ environment of developing alfalfa zygotic embryos is rich in ABA and low in osmotic potential.”
Claim · model-written summary
Early fluridone treatment produced seeds with low ABA.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- seedse0
- Treatment
- fluridonee0
- Outcome
- low amounts of ABAe0
- Direction
- lowe0
- Comparator
- untreated control seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When ABA synthesis was inhibited by treating the pods with fluridone at an early stage of development, the seeds which subsequently developed contained low amounts of ABA, but had a similar osmotic potential as untreated control seeds.”
t0“alfalfa”
Claim · model-written summary
Fluridone treated seeds had similar osmotic potential to untreated controls.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- fluridonee1
- Outcome
- osmotic potentiale0
- Direction
- similare0
- Comparator
- untreated control seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the seeds which subsequently developed contained low amounts of ABA, but had a similar osmotic potential as untreated control seeds.”
t0“alfalfa”
e1“treating the pods with fluridone”
Claim · model-written summary
Reduced ABA changed seed colour but not other morphology.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- fluridone-treated podse0
- Outcome
- morphology except the colour of seedse0
- Direction
- did not changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The reduced ABA in seeds from fluridone-treated pods did not change the morphology except the colour of seeds,”
t0“alfalfa”
Claim · model-written summary
Reduced ABA did not induce viviparous germination.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- fluridone-treated podse1
- Outcome
- viviparous germinatione0
- Direction
- nor did it inducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nor did it induce viviparous germination”
t0“alfalfa”
e1“seeds from fluridone-treated pods”
Claim · model-written summary
Reduced ABA did not affect storage protein synthesis.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- fluridone-treated podse1
- Outcome
- storage protein synthesise0
- Direction
- or affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“or affect storage protein synthesis.”
e1“seeds from fluridone-treated pods”
t0“alfalfa”
Claim · model-written summary
Two early to mid-development nonstorage proteins were absent from fluridone treated seeds.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- fluridone-treated seedse0
- Outcome
- two nonstorage proteinse0
- Direction
- absente0
- Comparator
- control seedse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, two nonstorage proteins which were synthesized in control seeds during early to mid-development were absent from fluridone-treated seeds.”
t0“alfalfa”
Claim · model-written summary
Control seeds with the two proteins were desiccation tolerant, whereas fluridone treated seeds lacking them were intolerant until storage protein deposition neared completion.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- desiccation-intolerante0
- Qualifier
- at least until the deposition of storage proteins was nearly completee0
- Comparator
- Control seedse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Control seeds containing these two proteins were desiccation-tolerant, whereas the fluridone-treated seeds which lacked them were desiccation-intolerant, at least until the deposition of storage proteins was nearly complete.”
t0“alfalfa”
Claim · model-written summary
Culture on nutrient medium induced embryo germination.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- isolated embryose0
- Treatment
- nutrient mediume0
- Outcome
- germinatione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culture of isolated embryos on nutrient medium induced germination and curtailed storage protein synthesis in the embryos.”
t0“alfalfa”
Claim · model-written summary
Culture on nutrient medium curtailed embryo storage protein synthesis.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- nutrient mediume0
- Outcome
- storage protein synthesise0
- Direction
- curtailede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Culture of isolated embryos on nutrient medium induced germination and curtailed storage protein synthesis in the embryos.”
t0“alfalfa”
Claim · model-written summary
ABA addition prevented germination and maintained storage protein synthesis.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- ABAe0
- Outcome
- germination and maintained storage protein synthesise0
- Direction
- preventede0
- Comparator
- nutrient mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of either ABA or osmoticum to the nutrient medium prevented germination and maintained storage protein synthesis.”
t0“alfalfa”
Claim · model-written summary
Osmoticum addition prevented germination and maintained storage protein synthesis.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- osmoticume0
- Outcome
- germination and maintained storage protein synthesise0
- Direction
- preventede0
- Comparator
- nutrient mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of either ABA or osmoticum to the nutrient medium prevented germination and maintained storage protein synthesis.”
t0“alfalfa”
Claim · model-written summary
Fluridone alongside osmoticum allowed germination.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- fluridone was added along with osmoticume0
- Outcome
- germination occurrede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When fluridone was added along with osmoticum, germination occurred, but storage protein synthesis was maintained.”
t0“alfalfa”
Claim · model-written summary
Fluridone alongside osmoticum maintained storage protein synthesis.
- Taxon
- alfalfaMedicago sativat0
- Treatment
- fluridone was added along with osmoticume0
- Outcome
- storage protein synthesise0
- Direction
- maintainede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When fluridone was added along with osmoticum, germination occurred, but storage protein synthesis was maintained.”
t0“alfalfa”
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See all 28 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.