Abstract
Incorporation of 35S into protein is reduced by exposure to Al in wheat (Triticum aestivum), but the effects are genotype-specific. Exposure to 10 to 75 [mu]M Al had little effect on 35S incorporation into total protein, nuclear and mitochondrial protein, microsomal protein, and cytosolic protein in the Al-resistant cultivar PT741. In contrast, 10 [mu]M Al reduced incorporation by 21 to 38% in the Al-sensitive cultivar Katepwa, with effects becoming more pronounced (31-62%) as concentrations of Al increased. We previously reported that a pair of 51-kD membrane-bound proteins accumulated in root tips of PT741 under conditions of Al stress. We now report that the 51-kD band is labeled with 35S after 24 h of exposure to 75 [mu]M Al. The specific induction of the 51-kD band in PT741 suggested a potential role of one or both of these proteins in mediating resistance to Al. Therefore, we analyzed their expression in single plants from an F2 population arising from a cross between the PT741 and Katepwa cultivars. Accumulation of 1,3-[beta]-glucans (callose) in root tips after 24 h of exposure to 100 [mu]M Al indicated that this population segregated for Al resistance in about a 3:1 ratio. A close correlation between resistance to Al (low callose content of root tips) and accumulation of the 51-kD band was observed, indicating that at least one of these proteins cosegregates with the Al-resistance phenotype. As a first step in identifying a possible function, we have demonstrated that the 51-kD band is most clearly associated with the tonoplast. Whereas Al has been reported to stimulate the activity of the tonoplast H+-ATPase and H+-PPase, antibodies raised against these proteins did not cross-react with the 51-kD band. Efforts are now under way to purify this protein from tonoplast-enriched fractions.
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Claims 7
Claim · model-written summary
Aluminium at 10 micromolar reduced protein labelling by 21 to 38 per cent in the Al-sensitive wheat cultivar Katepwa.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- Katepwae1
- Treatment
- 10 [mu]M Ale1
- Outcome
- incorporatione1
- Direction
- reducede1
- Value
- 21 to 38%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“10 [mu]M Al reduced incorporation by 21 to 38% in the Al-sensitive cultivar Katepwa”
t0“in a Segregating Population of Wheat”
Claim · model-written summary
Exposure to 10 to 75 micromolar Al had little effect on protein labelling in the Al-resistant wheat cultivar PT741.
- Taxon
- WheatTriticum aestivumt0
- Material
- PT741e1
- Treatment
- 10 to 75 [mu]M Ale1
- Outcome
- 35S incorporation into total protein, nuclear and mitochondrial protein, microsomal protein, and cytosolic proteine1
- Direction
- little effecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Exposure to 10 to 75 [mu]M Al had little effect on 35S incorporation into total protein, nuclear and mitochondrial protein, microsomal protein, and cytosolic protein in the Al-resistant cultivar PT741”
t0“in a Segregating Population of Wheat”
Claim · model-written summary
The 51-kD band was labelled with 35S after 24 h exposure to 75 micromolar Al in wheat.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- 75 [mu]M Ale1
- Outcome
- labeled with 35Se1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the 51-kD band is labeled with 35S after 24 h of exposure to 75 [mu]M Al”
t0“in a Segregating Population of Wheat”
Claim · model-written summary
Accumulation of the 51-kD band was closely correlated with Al resistance (low callose) in the F2 population.
- Taxon
- WheatTriticum aestivumt0
- Material
- F2 populatione2
- Outcome
- accumulation of the 51-kD bande1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A close correlation between resistance to Al (low callose content of root tips) and accumulation of the 51-kD band was observed”
e2“we analyzed their expression in single plants from an F2 population arising from a cross between the PT741 and Katepwa cultivars”
t0“in a Segregating Population of Wheat”
Claim · model-written summary
The F2 population segregated for Al resistance in about a 3:1 ratio.
- Taxon
- WheatTriticum aestivumt0
- Outcome
- segregated for Al resistancee1
- Value
- about a 3:1 ratioe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“this population segregated for Al resistance in about a 3:1 ratio”
t0“in a Segregating Population of Wheat”
Claim · model-written summary
The 51-kD band is most clearly associated with the tonoplast.
- Taxon
- WheatTriticum aestivumt0
- Gene
- 51-kD bande1
- Outcome
- most clearly associated with the tonoplaste1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“the 51-kD band is most clearly associated with the tonoplast”
t0“in a Segregating Population of Wheat”
Claim · model-written summary
Antibodies against the tonoplast H+-ATPase and H+-PPase did not cross-react with the 51-kD band.
- Taxon
- WheatTriticum aestivumt0
- Gene
- H+-ATPase and H+-PPasee2
- Outcome
- cross-react with the 51-kD bande1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“antibodies raised against these proteins did not cross-react with the 51-kD band”
e2“Whereas Al has been reported to stimulate the activity of the tonoplast H+-ATPase and H+-PPase”
t0“in a Segregating Population of Wheat”
The same questions in other papers
aluminium atom · quality: protein · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.