Abstract
Soybean mosaic virus (SMV), belonging to the genus Potyvirus of the family Potyviridae, has a relatively narrow host range almost exclusively confined to leguminous hosts. While disease management through genetic transformation can be an effective approach, soybean remains recalcitrant to routine genetic transformation. In this context, it is important to identify new hosts for SMV that can be used to develop effective transgenic resistance strategies. Transformation in Nicotiana benthamiana is simple and highly efficient; hence, here we demonstrate the infectivity of SMV strain SC7 in N. benthamiana plants. To identify an SMV strain infectious in N. benthamiana, we mechanically inoculated N. benthamiana plants with 37 isolates from 21 (SC1 to SC21) SMV strains. Plants inoculated with isolates of strain SC7 produced mosaic symptoms on leaves. However, N. benthamiana plants inoculated with the 20 other SMV strains showed no visible symptoms. Furthermore, soybean cv. Nannong 1138-2 inoculated with sap prepared from symptomatic N. benthamiana leaves showed typical SMV mosaic symptoms 2 weeks after inoculation. In addition, SMV was detected in symptomatic N. benthamiana and soybean leaves by RT-PCR, DAS-ELISA, and further identified by sequencing. Together, the results indicate that N. benthamiana plants could support multiplication of SMV strain SC7. The findings of this study would be useful for the investigation of SMV resistance using the model plant N. benthamiana.
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Claims 6
Claim · model-written summary
SMV strain SC7 caused leaf mosaic symptoms in inoculated Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- inoculated with isolates of strain SC7e0
- Interactor
- SMV strain SC7e1pathogen
- Outcome
- mosaic symptoms on leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plants inoculated with isolates of strain SC7 produced mosaic symptoms on leaves.”
t0“Nicotiana benthamiana”
e1“SMV strain SC7 in N. benthamiana plants.”
Claim · model-written summary
Inoculation with the other 20 SMV strains gave no visible symptoms in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- inoculated with the 20 other SMV strainse0
- Interactor
- SMVe0pathogen
- Outcome
- no visible symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N. benthamiana plants inoculated with the 20 other SMV strains showed no visible symptoms.”
Claim · model-written summary
Sap from symptomatic N. benthamiana leaves produced typical SMV mosaic symptoms in soybean cv. Nannong 1138-2 after two weeks.
- Taxon
- soybean
Glycine maxe0 - Material
- Nannong 1138-2e0
- Treatment
- inoculated with sap prepared from symptomatic N. benthamiana leavese0
- Interactor
- SMVe0pathogen
- Outcome
- typical SMV mosaic symptomse0
- Qualifier
- 2 weeks after inoculatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean cv. Nannong 1138-2 inoculated with sap prepared from symptomatic N. benthamiana leaves showed typical SMV mosaic symptoms 2 weeks after inoculation.”
Claim · model-written summary
SMV was detected in symptomatic N. benthamiana leaves by RT-PCR, DAS-ELISA and sequencing.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Organ / stage
- leavese0
- Interactor
- SMVe0pathogen
- Outcome
- SMV was detected in symptomatic N. benthamianae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SMV was detected in symptomatic N. benthamiana and soybean leaves by RT-PCR, DAS-ELISA, and further identified by sequencing.”
Claim · model-written summary
SMV was detected in symptomatic soybean leaves by RT-PCR, DAS-ELISA and sequencing.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- leavese0
- Interactor
- SMVe0pathogen
- Outcome
- SMV was detected in symptomatic N. benthamiana and soybean leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SMV was detected in symptomatic N. benthamiana and soybean leaves by RT-PCR, DAS-ELISA, and further identified by sequencing.”
Claim · model-written summary
N. benthamiana plants could support multiplication of SMV strain SC7.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- SMV strain SC7e0pathogen
- Outcome
- support multiplication of SMV strain SC7e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N. benthamiana plants could support multiplication of SMV strain SC7.”
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See all 127 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.