Abstract
Five-mm sections of elongation zones which were cut from primary roots or coleoptiles of Zea mays were incubated for designated periods with various concentrations of IAA. In vitro protein phosphorylation in the soluble fractionsprepared from these sections was analyzed by SDS-PAGE. The phosphorylation of proteins in sections of primary roots incubated for 20 or 40 min in the presenceof 10^-7 M IAA was greater than that in the sections incubated in the absence ofIAA. The phosphorylation of proteins in sections of primary roots incubated for 20 min or 2h in the presence of 10^-8,10^-7 or 10^-6 M IAA was higher than that in the sections incubated in the absence of IAA. An incuba-tion for 20 min or 2h with 10^-4 M IAA inhibited the phosphorylation of proteins in sections of primary roots. The growth of the sections of primary roots incubated for 2h in the presence of 10^-7 M IAA or higher concentrations was lower than that of the sections incubated in the absence of IAA. These results suggest that the phosphorylation of proteins which was increased by IAA treatment is independent of an inhibition of the growth induced by IAA in maize primary roots. The phosphorylation of proteins in sections of coleoptiles incubated for 10, 20 or 40 min in the presence of 10^-7 or 10^-5 M IAA was equal to that in the sectionsincubated in the absence of IAA. These results show that IAA regulates growth ofmaize coleoptiles in no relation with phosphorylation of the proteins and that IAA regulates growth of maize mesocotyls via phosphorylation of the proteins, comparing with earlier results [Kato et al. (1996) Plant Cell Phsiol. 37:667]. Coleoptile―IAA treatment―Primary root―Protein phosphorylation―Zea mays.
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Claims 5
Claim · model-written summary
IAA at 10^-7 M increased root protein phosphorylation after 20 or 40 min.
- Organ / stage
- primary rootse0
- Treatment
- 10^-7 M IAAe0
- Outcome
- phosphorylation of proteinse0
- Direction
- greatere0
- Qualifier
- 20 or 40 mine0
- Comparator
- absence ofIAAe0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phosphorylation of proteins in sections of primary roots incubated for 20 or 40 min in the presenceof 10^-7 M IAA was greater than that in the sections incubated in the absence ofIAA.”
t0“Zea mays”
Claim · model-written summary
IAA at 10^-8 to 10^-6 M increased root protein phosphorylation after 20 min or 2h.
- Organ / stage
- primary rootse0
- Treatment
- 10^-8,10^-7 or 10^-6 M IAAe0
- Outcome
- phosphorylation of proteinse0
- Direction
- highere0
- Qualifier
- 20 min or 2he0
- Comparator
- absence of IAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phosphorylation of proteins in sections of primary roots incubated for 20 min or 2h in the presence of 10^-8,10^-7 or 10^-6 M IAA was higher than that in the sections incubated in the absence of IAA.”
t0“Zea mays”
Claim · model-written summary
IAA at 10^-4 M inhibited root protein phosphorylation.
- Organ / stage
- primary rootse0
- Treatment
- 10^-4 M IAAe0
- Outcome
- phosphorylation of proteinse0
- Direction
- inhibitede0
- Qualifier
- 20 min or 2he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An incuba-tion for 20 min or 2h with 10^-4 M IAA inhibited the phosphorylation of proteins in sections of primary roots.”
t0“Zea mays”
Claim · model-written summary
IAA at 10^-7 M or higher reduced root-section growth after 2h.
- Organ / stage
- primary rootse0
- Treatment
- 10^-7 M IAA or higher concentrationse0
- Outcome
- growthe0
- Direction
- lowere0
- Qualifier
- 2he0
- Comparator
- absence of IAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of the sections of primary roots incubated for 2h in the presence of 10^-7 M IAA or higher concentrations was lower than that of the sections incubated in the absence of IAA.”
t0“Zea mays”
Claim · model-written summary
IAA at 10^-7 or 10^-5 M left coleoptile protein phosphorylation equal to untreated sections.
- Organ / stage
- coleoptilese0
- Treatment
- 10^-7 or 10^-5 M IAAe0
- Outcome
- phosphorylation of proteinse0
- Direction
- equale0
- Qualifier
- 10, 20 or 40 mine0
- Comparator
- absence of IAAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phosphorylation of proteins in sections of coleoptiles incubated for 10, 20 or 40 min in the presence of 10^-7 or 10^-5 M IAA was equal to that in the sectionsincubated in the absence of IAA.”
t0“Zea mays”
The same questions in other papers
indole-3-acetic acid · molecular: expression · Zea mays (maize)Positive and negative results
3 in the opposite direction
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.