Abstract
Five-mm sections of elongation zones of Zea mesocotyls were incubated for designated periods with various concentrations of IAA. In vitro protein phosphorylation in the soluble fraction (85,000 × g supernatant) prepared from the sections was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The phosphorylation of proteins in the soluble fraction that had been prepared from sections incubated for 20 min in the presence of 10˜s M IAA was greater than that in the sections incubated for 20 min without IAA. The amount of phosphorylation of proteins per protein became higher when higher concentrations increased (10˜8—10˜5M). The growth of sections incubated in the presence of 10˜8 M IAA or higher concentrations was greater than that of sections incubated in the absence of IAA. The promotion of growth by IAA was greater at higher concentrations of IAA. Proteins in the soluble fraction, prepared from sections incubated for 20 min in the presence of 10˜5 M IAA, were phosphorylated in the presence of either 10 fM cAMP, 10 μM cGMP, 100 μM W-7, 100 μM W-5, 20 μM H-7 or 20 μM HA1004. The calmodulin antagonist, W-7, and the inhibitor of protein kinase C, H-7, inhibited the phosphorylation of proteins stimulated by incubation with IAA. These results suggest that IAA promotes cell elongation via protein phosphorylation that depends on calmodulin-dependent protein kinase and protein kinase C.
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Claims 7
Claim · model-written summary
Protein phosphorylation in the soluble fraction was greater in mesocotyl sections incubated 20 min with IAA than in sections without IAA.
- Organ / stage
- Mesocotylst0
- Treatment
- IAAe1
- Outcome
- phosphorylation of proteinse0
- Direction
- greatere1
- Comparator
- sections incubated for 20 min without IAAe1
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The phosphorylation of proteins in the soluble fraction”
e1“was greater than that in the sections incubated for 20 min without IAA”
t0“Mesocotyls in Zea mays”
Claim · model-written summary
Phosphorylation of proteins per protein became higher as IAA concentrations increased.
- Organ / stage
- Mesocotylst0
- Treatment
- higher concentrationse0
- Outcome
- phosphorylation of proteins per proteine0
- Direction
- became highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amount of phosphorylation of proteins per protein became higher when higher concentrations increased”
t0“Mesocotyls in Zea mays”
Claim · model-written summary
Growth of mesocotyl sections with IAA was greater than that of sections without IAA.
- Organ / stage
- Mesocotylst0
- Treatment
- IAAe1
- Outcome
- growth of sectionse0
- Direction
- greatere1
- Comparator
- sections incubated in the absence of IAAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The growth of sections”
e1“was greater than that of sections incubated in the absence of IAA”
t0“Mesocotyls in Zea mays”
Claim · model-written summary
The promotion of growth by IAA was greater at higher IAA concentrations.
- Organ / stage
- Mesocotylst0
- Treatment
- higher concentrations of IAAe0
- Outcome
- promotion of growthe0
- Direction
- greatere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promotion of growth by IAA was greater at higher concentrations of IAA”
t0“Mesocotyls in Zea mays”
Claim · model-written summary
The calmodulin antagonist W-7 inhibited the IAA-stimulated phosphorylation of proteins.
- Organ / stage
- Mesocotylst0
- Treatment
- W-7e0
- Outcome
- phosphorylation of proteins stimulated by incubation with IAAe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The calmodulin antagonist, W-7, and the inhibitor of protein kinase C, H-7, inhibited the phosphorylation of proteins stimulated by incubation with IAA”
t0“Mesocotyls in Zea mays”
Claim · model-written summary
The protein kinase C inhibitor H-7 inhibited the IAA-stimulated phosphorylation of proteins.
- Organ / stage
- Mesocotylst0
- Treatment
- H-7e0
- Outcome
- phosphorylation of proteins stimulated by incubation with IAAe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The calmodulin antagonist, W-7, and the inhibitor of protein kinase C, H-7, inhibited the phosphorylation of proteins stimulated by incubation with IAA”
t0“Mesocotyls in Zea mays”
Claim · model-written summary
The authors suggest IAA promotes cell elongation via protein phosphorylation dependent on calmodulin-dependent protein kinase and protein kinase C.
- Treatment
- IAAe0
- Gene
- calmodulin-dependent protein kinase and protein kinase Ce0
- Outcome
- cell elongatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA promotes cell elongation via protein phosphorylation that depends on calmodulin-dependent protein kinase and protein kinase C”
t0“Mesocotyls in Zea mays”
The same questions in other papers
indole-3-acetic acid · growth · Zea mays (maize)Results differ
34 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- no effect
37 papers more in this group
See all 41 papers →indole-3-acetic acid · molecular: expression · Zea mays (maize)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.