Abstract
We have isolated four genomic DNA clones that contain the transcription initiation site of the parA gene(s) from a tobacco genomic library by hybridization with the 5' segment of the parA cDNA previously isolated. They were classified into two types on the basis of their nucleotide sequences. Southern blot analysis indicated that two types of clones were respectively derived from the two parental species of tobacco, Nicotiana tomentosiformis and Nicotiana sylvestris. The genes corresponding to these clones were designated as parAt and parAs, respectively, and the parA cDNA clone was shown to code for mRNA from parAt on the basis of its nucleotide sequence. The 5' regions about 400 nucleotides upstream from the transcription initiation sites of the parAt and parAs genomic clones were highly homologous to one another, but regions further upstream showed no significant similarity. The coding sequence of the GUS (beta-glucuronidase) reporter gene was linked to the 5'-upstream regions of parAt and parAs, and the sites of expression of these fusion genes were examined in transgenic tobacco plants. In the absence of auxins, both fusion genes were expressed in capsules at a late stage of seed development, mature seeds, a root apex and a root-hair zone whereas no significant expression was seen in other organs. Their expression was enhanced by 2,4-dichlorophenoxyacetic acid in most of the organs of tobacco. The results show that expression of these genes is regulated by both organ-specific and auxin-inducible mechanisms.
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Claims 6
Claim · model-written summary
Four tobacco genomic clones fell into two sequence types.
- Taxon
- Tobacco
Nicotiana tabacumt0 - Gene
- par At0
- Outcome
- two typese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They were classified into two types on the basis of their nucleotide sequences.”
t0“Tobacco par A”
Claim · model-written summary
The two clone types derived from the two parental species of tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- derived from the two parental species of tobaccoe0
- Qualifier
- Nicotiana tomentosiformis and Nicotiana sylvestrise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two types of clones were respectively derived from the two parental species of tobacco, Nicotiana tomentosiformis and Nicotiana sylvestris”
Claim · model-written summary
The parA cDNA encoded mRNA from parAt.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- parAte0
- Outcome
- mRNA from parAte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the parA cDNA clone was shown to code for mRNA from parAt on the basis of its nucleotide sequence”
t0“Tobacco”
Claim · model-written summary
The proximal upstream regions of parAt and parAs were highly homologous, while farther upstream regions lacked significant similarity.
- Taxon
- TobaccoNicotiana tabacumt0
- Gene
- parAt and parAse0
- Outcome
- highly homologous to one anothere0
- Value
- about 400 nucleotidese0
- Qualifier
- regions further upstream showed no significant similaritye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The 5' regions about 400 nucleotides upstream from the transcription initiation sites of the parAt and parAs genomic clones were highly homologous to one another, but regions further upstream showed no significant similarity.”
t0“Tobacco”
Claim · model-written summary
Without auxins, both fusion genes were expressed in late capsules, mature seeds, root apex and root-hair zone.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- capsules at a late stage of seed development, mature seeds, a root apex and a root-hair zonee0
- Outcome
- both fusion genes were expressede0
- Qualifier
- no significant expression was seen in other organse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the absence of auxins, both fusion genes were expressed in capsules at a late stage of seed development, mature seeds, a root apex and a root-hair zone whereas no significant expression was seen in other organs.”
t0“Tobacco”
Claim · model-written summary
2,4-dichlorophenoxyacetic acid enhanced fusion gene expression in most tobacco organs.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- most of the organs of tobaccoe0
- Treatment
- 2,4-dichlorophenoxyacetic acide0
- Outcome
- expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Their expression was enhanced by 2,4-dichlorophenoxyacetic acid in most of the organs of tobacco.”
The same questions in other papers
2,4-D · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.