Abstract
Multispectral analysis combined with the Periodic Acid-Schiff method was used to investigate cytological features of insoluble polysaccharides and changes in total insoluble polysaccharide content (TPC) during pollen development in rice, including four cytoplasmic male sterility lines (MSLs) and their corresponding fertility-maintaining lines (FMLs). The multispectral curves of the relative transmittance value (RTV) and the images of developing pollen cells were obtained across a range of successive wavelengths (400-720 nm). A minimum RTV was found near 550 nm indicating an absorption peak of the TPC. Thus, the TPC was measured using the RTV of 550 nm. In the four FMLs, the minimum TPC of developing pollen cells occurred at the late microspore stage, while the maximum TPC occurred at the mature pollen grain stage. The TPC levels of pollen cells were significantly higher in the four FMLs than in their corresponding MSLs during and after pollen abortion. Notably, a steep decrease of multispectral curves at 420 nm appeared before the occurrence of abortion, implying a marker associated with pollen abortion in rice. Our results will be helpful for exploring the changes in TPC during pollen ontogenesis in rice and provide a novel method for the study of bio-macromolecules.
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Claims 5
Claim · model-written summary
Relative transmittance reached a minimum near 550 nm, indicating peak absorption of total insoluble polysaccharides.
- Taxon
- rice
Oryza sativat0 - Outcome
- minimum RTVe0
- Value
- 550 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A minimum RTV was found near 550 nm indicating an absorption peak of the TPC”
t0“Rice”
Claim · model-written summary
In fertility maintaining lines, pollen TPC was lowest at late microspore stage.
- Taxon
- riceOryza sativat0
- Material
- FMLse0
- Organ / stage
- late microspore stagee0
- Outcome
- minimum TPCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In the four FMLs, the minimum TPC of developing pollen cells occurred at the late microspore stage”
t0“Rice”
Claim · model-written summary
In fertility maintaining lines, pollen TPC was highest at mature pollen stage.
- Taxon
- riceOryza sativat0
- Organ / stage
- mature pollen grain stagee0
- Outcome
- maximum TPCe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the maximum TPC occurred at the mature pollen grain stage”
t0“Rice”
Claim · model-written summary
Pollen TPC was significantly higher in fertility maintaining lines than male sterile lines during and after abortion.
- Taxon
- riceOryza sativat0
- Material
- FMLse0
- Outcome
- TPC levels of pollen cellse0
- Direction
- highere0
- Qualifier
- during and after pollen abortione0
- Comparator
- MSLse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TPC levels of pollen cells were significantly higher in the four FMLs than in their corresponding MSLs during and after pollen abortion”
t0“Rice”
Claim · model-written summary
A curve decrease at 420 nm preceded pollen abortion, suggesting a marker.
- Taxon
- riceOryza sativae0
- Outcome
- marker associated with pollen abortione0
- Direction
- decreasee0
- Value
- 420 nme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a steep decrease of multispectral curves at 420 nm appeared before the occurrence of abortion, implying a marker associated with pollen abortion in rice”