Abstract
Cucumber mosaic virus (CMV) is known to systemically infect Arabidopsis thaliana ecotype Columbia plants. In order to identify the host factors involved in the multiplication of CMV, we isolated an A. thaliana mutant in which the accumulation of the coat protein (CP) of CMV in upper uninoculated leaves was delayed. Genetic analyses suggested that the phenotype of delayed accumulation of CMV CP in the mutant plants was caused by a single, nuclear and recessive mutation designated cum1-1, which was located on chromosome IV. The cum1-1 mutation did not affect the multiplication of tobacco mosaic virus, turnip crinkle virus or turnip yellow mosaic virus, which belong to different taxonomic groups from CMV. Accumulation of CMV CP in the inoculated leaves of cum1-1 plants was also delayed either when CMV virion or CMV virion RNA was inoculated. On the other hand, when cum1-1 and the wild-type Col-0 protoplasts were inoculated with CMV virion RNA by electroporation, the accumulations of CMV-related RNAs and the coat protein were similar. These results suggest that the cum1-1 mutation did not affect the uncoating of CMV virion and subsequent replication in an initially infected cell but affected the spreading of CMV within an infected leaf, possibly the cell-to-cell movement of CMV in a virus-specific manner.
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Claims 9
Claim · model-written summary
CMV coat protein accumulated later in upper uninoculated mutant leaves.
- Taxon
- A. thaliana
Arabidopsis thalianae0 - Material
- mutante0
- Organ / stage
- upper uninoculated leavese0
- Interactor
- CMV
cucumber mosaic cucumoviruse0pathogen - Outcome
- accumulation of the coat protein (CP) of CMVe0
- Direction
- delayede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated an A. thaliana mutant in which the accumulation of the coat protein (CP) of CMV in upper uninoculated leaves was delayed.”
Claim · model-written summary
Delayed CMV coat protein accumulation was associated with a single recessive nuclear mutation on chromosome IV.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Gene
- cum1-1e0
- Outcome
- delayed accumulation of CMV CPe0
- Direction
- delayede0
- Qualifier
- chromosome IVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the phenotype of delayed accumulation of CMV CP in the mutant plants was caused by a single, nuclear and recessive mutation designated cum1-1, which was located on chromosome IV.”
t0“Arabidopsis thaliana”
Claim · model-written summary
The mutation did not affect tobacco mosaic virus multiplication.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cum1-1 mutatione0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Gene
- cum1-1e0
- Outcome
- multiplication of tobacco mosaic viruse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cum1-1 mutation did not affect the multiplication of tobacco mosaic virus, turnip crinkle virus or turnip yellow mosaic virus”
t0“Arabidopsis thaliana”
Claim · model-written summary
The mutation did not affect turnip crinkle virus multiplication.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cum1-1 mutatione0
- Interactor
- turnip crinkle virusTurnip crinkle viruse0pathogen
- Gene
- cum1-1e0
- Outcome
- turnip crinkle viruse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cum1-1 mutation did not affect the multiplication of tobacco mosaic virus, turnip crinkle virus or turnip yellow mosaic virus”
t0“Arabidopsis thaliana”
Claim · model-written summary
The mutation did not affect turnip yellow mosaic virus multiplication.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- cum1-1 mutatione0
- Interactor
- turnip yellow mosaic virus
Turnip yellow mosaic viruse0pathogen - Gene
- cum1-1e0
- Outcome
- turnip yellow mosaic viruse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cum1-1 mutation did not affect the multiplication of tobacco mosaic virus, turnip crinkle virus or turnip yellow mosaic virus”
t0“Arabidopsis thaliana”
Claim · model-written summary
CMV coat protein accumulation in inoculated cum1-1 leaves was delayed after virion inoculation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cum1-1 plantse0
- Treatment
- CMV virione0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- Accumulation of CMV CPe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of CMV CP in the inoculated leaves of cum1-1 plants was also delayed either when CMV virion or CMV virion RNA was inoculated.”
t0“Arabidopsis thaliana”
Claim · model-written summary
CMV coat protein accumulation in inoculated cum1-1 leaves was delayed after viral RNA inoculation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cum1-1 plantse0
- Treatment
- CMV virion RNAe0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- Accumulation of CMV CPe0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of CMV CP in the inoculated leaves of cum1-1 plants was also delayed either when CMV virion or CMV virion RNA was inoculated.”
t0“Arabidopsis thaliana”
Claim · model-written summary
CMV-related RNA and coat protein accumulated similarly in mutant and wild-type protoplasts after RNA electroporation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- cum1-1e0
- Treatment
- CMV virion RNA by electroporatione0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Outcome
- accumulations of CMV-related RNAs and the coat proteine0
- Direction
- similare0
- Comparator
- wild-type Col-0 protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when cum1-1 and the wild-type Col-0 protoplasts were inoculated with CMV virion RNA by electroporation, the accumulations of CMV-related RNAs and the coat protein were similar.”
t0“Arabidopsis thaliana”
Claim · model-written summary
The authors inferred that cum1-1 affects within-leaf CMV spread, possibly cell-to-cell movement.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Interactor
- CMVcucumber mosaic cucumoviruse0pathogen
- Gene
- cum1-1e0
- Outcome
- spreading of CMV within an infected leafe0
- Direction
- affectede0
- Qualifier
- possibly the cell-to-cell movement of CMVe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cum1-1 mutation did not affect the uncoating of CMV virion and subsequent replication in an initially infected cell but affected the spreading of CMV within an infected leaf, possibly the cell-to-cell movement of CMV in a virus-specific manner.”
t0“Arabidopsis thaliana”
The same questions in other papers
Cucumis sativus · cucumber mosaic cucumovirus · Arabidopsis thaliana (thale cress)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.