Abstract
The viral genome-linked protein, VPg, of potyviruses is involved in viral genome replication and translation. To determine host proteins that interact with Sugarcane mosaic virus (SCMV) VPg, a yeast two-hybrid screen was used and a maize (Zea mays) Elongin C (ZmElc) protein was identified. ZmELC transcript was observed in all maize organs, but most highly in leaves and pistil extracts, and ZmElc was present in the cytoplasm and nucleus of maize cells in the presence or absence of SCMV. ZmELC expression was increased in maize tissue at 4 and 6 d post SCMV inoculation. When ZmELC was transiently overexpressed in maize protoplasts the accumulation of SCMV RNA was approximately doubled compared with the amount of virus in control protoplasts. Silencing ZmELC expression using a Brome mosaic virus-based gene silencing vector (virus-induced gene silencing) did not influence maize plant growth and development, but did decrease RNA accumulation of two isolates of SCMV and host transcript encoding ZmeIF4E during SCMV infection. Interestingly, Maize chlorotic mottle virus, from outside the Potyviridae, was increased in accumulation after silencing ZmELC expression. Our results describe both the location of ZmElc expression in maize and a new activity associated with an Elc: support of potyvirus accumulation.
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Claims 9
Claim · model-written summary
Maize Elongin C was identified as a protein interacting with SCMV VPg.
- Interactor
- Sugarcane mosaic virus (SCMV)
Sugarcane mosaic viruse0pathogen - Gene
- Elongin C (ZmElc)e0
- Outcome
- protein was identifiede0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“To determine host proteins that interact with Sugarcane mosaic virus (SCMV) VPg, a yeast two-hybrid screen was used and a maize (Zea mays) Elongin C (ZmElc) protein was identified”
Claim · model-written summary
ZmELC transcript was present in every maize organ and highest in leaves and pistils.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmELC transcript was observed in all maize organs, but most highly in leaves and pistil extracts”
Claim · model-written summary
ZmElc was present in the cytoplasm and nucleus with or without SCMV.
- Interactor
- SCMVSugarcane mosaic viruse0pathogen
- Gene
- ZmElce0
- Outcome
- present in the cytoplasm and nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmElc was present in the cytoplasm and nucleus of maize cells in the presence or absence of SCMV”
Claim · model-written summary
SCMV inoculation increased ZmELC expression at 4 and 6 days.
- Treatment
- SCMV inoculatione0
- Interactor
- SCMVSugarcane mosaic viruse0pathogen
- Gene
- ZmELCe0
- Outcome
- ZmELC expressione0
- Direction
- increasede0
- Qualifier
- 4 and 6 d post SCMV inoculatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmELC expression was increased in maize tissue at 4 and 6 d post SCMV inoculation”
Claim · model-written summary
Transient ZmELC overexpression approximately doubled SCMV RNA accumulation in maize protoplasts.
- Treatment
- ZmELC was transiently overexpressede0
- Interactor
- SCMVSugarcane mosaic viruse0pathogen
- Gene
- ZmELCe0
- Outcome
- accumulation of SCMV RNAe0
- Direction
- doublede0
- Value
- approximately doublede0
- Comparator
- control protoplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When ZmELC was transiently overexpressed in maize protoplasts the accumulation of SCMV RNA was approximately doubled compared with the amount of virus in control protoplasts”
Claim · model-written summary
Silencing ZmELC did not influence maize growth and development.
- Treatment
- Silencing ZmELC expressione0
- Gene
- ZmELCe0
- Outcome
- maize plant growth and developmente0
- Direction
- did not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing ZmELC expression using a Brome mosaic virus-based gene silencing vector (virus-induced gene silencing) did not influence maize plant growth and development”
Claim · model-written summary
Silencing ZmELC decreased RNA accumulation of two SCMV isolates.
- Interactor
- SCMVSugarcane mosaic viruse0pathogen
- Outcome
- RNA accumulation of two isolates of SCMVe0
- Direction
- decreasee0
Not reportedGeneTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did decrease RNA accumulation of two isolates of SCMV”
t0“Maize”
Claim · model-written summary
Silencing ZmELC decreased ZmeIF4E transcript during SCMV infection.
- Interactor
- SCMVSugarcane mosaic viruse0pathogen
- Gene
- ZmeIF4Ee0
- Outcome
- host transcript encoding ZmeIF4Ee0
- Direction
- decreasee0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“did decrease RNA accumulation of two isolates of SCMV and host transcript encoding ZmeIF4E during SCMV infection”
t0“Maize”
Claim · model-written summary
Silencing ZmELC increased Maize chlorotic mottle virus accumulation.
- Treatment
- silencing ZmELC expressione0
- Interactor
- Maize chlorotic mottle virusMaize chlorotic mottle viruse0pathogen
- Gene
- ZmELCe0
- Outcome
- Maize chlorotic mottle virus, from outside the Potyviridae, was increased in accumulatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize chlorotic mottle virus, from outside the Potyviridae, was increased in accumulation after silencing ZmELC expression”
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.