Abstract
Pathogens have evolved various strategies to overcome host immunity for successful infection. Maize chlorotic mottle virus (MCMV) can cause lethal necrosis in maize (Zea mays) when it coinfects with a virus in the Potyviridae family. However, the MCMV pathogenicity determinant remains largely unknown. Here we show that the P31 protein of MCMV is important for viral accumulation and essential for symptom development. Ectopic expression of P31 using foxtail mosaic virus or potato virus X induced necrosis in systemically infected maize or Nicotiana benthamiana leaves. Maize catalases (CATs) were shown to interact with P31 in yeast and in planta. P31 accumulation was elevated through its interaction with ZmCAT1. P31 attenuated the expression of salicylic acid (SA)-responsive pathogenesis-related (PR) genes by inhibiting catalase activity during MCMV infection. In addition, silencing of ZmCATs using a brome mosaic virus-based gene silencing vector facilitated MCMV RNA and coat protein accumulation. This study reveals an important role for MCMV P31 in counteracting host defence and inducing systemic chlorosis and necrosis. Our results have implications for understanding the mechanisms in defence and counter-defence during infection of plants by various pathogens.
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Claims 9
Claim · model-written summary
MCMV P31 was important for viral accumulation.
- Interactor
- MCMVMaize chlorotic mottle viruse0pathogen
- Gene
- P31 protein of MCMVe0
- Outcome
- viral accumulatione0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the P31 protein of MCMV is important for viral accumulation”
e1“maize (Zea mays)”
Claim · model-written summary
MCMV P31 was essential for symptom development.
- Interactor
- MCMVMaize chlorotic mottle viruse0pathogen
- Gene
- P31 protein of MCMVe0
- Outcome
- symptom developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P31 protein of MCMV is important for viral accumulation and essential for symptom development”
e1“maize (Zea mays)”
Claim · model-written summary
Ectopic P31 expression using foxtail mosaic virus induced leaf necrosis in maize.
- Organ / stage
- leavese0
- Treatment
- Ectopic expression of P31 using foxtail mosaic viruse0
- Interactor
- foxtail mosaic virusFoxtail mosaic viruse0pathogen
- Gene
- P31e0
- Outcome
- necrosise0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of P31 using foxtail mosaic virus or potato virus X induced necrosis in systemically infected maize or Nicotiana benthamiana leaves”
Claim · model-written summary
Ectopic P31 expression using potato virus X induced leaf necrosis in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Organ / stage
- leavese0
- Treatment
- potato virus Xe0
- Interactor
- potato virus XPotato virus Xe0pathogen
- Gene
- P31e0
- Outcome
- necrosise0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of P31 using foxtail mosaic virus or potato virus X induced necrosis in systemically infected maize or Nicotiana benthamiana leaves”
Claim · model-written summary
Maize catalases interacted with P31 in yeast and plants.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize catalases (CATs) were shown to interact with P31 in yeast and in planta”
Claim · model-written summary
Interaction with ZmCAT1 elevated P31 accumulation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P31 accumulation was elevated through its interaction with ZmCAT1”
e1“maize (Zea mays)”
Claim · model-written summary
P31 inhibited catalase activity and attenuated SA-responsive PR gene expression during infection.
- Interactor
- MCMVMaize chlorotic mottle viruse0pathogen
- Gene
- pathogenesis-related (PR) genese0
- Outcome
- expression of salicylic acid (SA)-responsive pathogenesis-related (PR) genese0
- Direction
- attenuatede0
- Qualifier
- by inhibiting catalase activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“P31 attenuated the expression of salicylic acid (SA)-responsive pathogenesis-related (PR) genes by inhibiting catalase activity during MCMV infection”
e1“maize (Zea mays)”
Claim · model-written summary
ZmCAT silencing facilitated MCMV RNA accumulation.
- Treatment
- silencing of ZmCATse0
- Interactor
- MCMVMaize chlorotic mottle viruse0pathogen
- Gene
- ZmCATse0
- Outcome
- MCMV RNAe0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of ZmCATs using a brome mosaic virus-based gene silencing vector facilitated MCMV RNA and coat protein accumulation”
e1“maize (Zea mays)”
Claim · model-written summary
ZmCAT silencing facilitated MCMV coat protein accumulation.
- Treatment
- silencing of ZmCATse0
- Interactor
- MCMVMaize chlorotic mottle viruse0pathogen
- Gene
- ZmCATse0
- Outcome
- MCMV RNA and coat protein accumulatione0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of ZmCATs using a brome mosaic virus-based gene silencing vector facilitated MCMV RNA and coat protein accumulation”
e1“maize (Zea mays)”
The same questions in other papers
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- positive
- positive
- negative
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See all 25 papers →loss of function · Maize chlorotic mottle virus · Zea mays (maize)Results agree
4 papers in the same direction as this paper
- negative
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.