Abstract
Maize chlorotic mottle virus (MCMV) is one of the main viruses causing significant losses in maize. N 6 -methyladenosine (m 6 A) RNA modification has been proven to play important regulatory roles in plant development and stress response. In this study, we found that MCMV infection significantly up-regulated the m 6 A level in maize, and methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) were performed to investigate the distribution of m 6 A modified peaks and gene expression patterns in MCMV-infected maize plants. The results showed that 1325 differentially methylated genes (DMGs) and 47 differentially methylated and expressed genes (DMEGs) were identified and analyzed. Moreover, the results of virus-induced gene silencing (VIGS) assays showed that ZmECT18 and ZmGST31 were required for MCMV infection, while silencing of ZmMTC, ZmSCI1 or ZmTIP1 significantly promoted MCMV infection in maize. Our findings provided novel insights into the regulatory roles of m 6 A modification in maize response to MCMV infection.
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Claims 5
Claim · model-written summary
MCMV infection significantly up-regulated the m6A level in maize.
- Treatment
- MCMV infectione1
- Interactor
- MCMVMaize chlorotic mottle viruse1pathogen
- Outcome
- m 6 A levele1
- Direction
- up-regulatede1
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we found that MCMV infection significantly up-regulated the m 6 A level in maize”
Claim · model-written summary
1325 differentially methylated genes were identified in MCMV-infected maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize chlorotic mottle virus (MCMV) is one of the main viruses causing significant losses in maize”
e2“The results showed that 1325 differentially methylated genes (DMGs) and 47 differentially methylated and expressed genes (DMEGs) were identified and analyzed”
Claim · model-written summary
47 differentially methylated and expressed genes were identified in MCMV-infected maize.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maize chlorotic mottle virus (MCMV) is one of the main viruses causing significant losses in maize”
e2“The results showed that 1325 differentially methylated genes (DMGs) and 47 differentially methylated and expressed genes (DMEGs) were identified and analyzed”
Claim · model-written summary
ZmECT18 and ZmGST31 were required for MCMV infection in maize.
- Treatment
- virus-induced gene silencing (VIGS)e3
- Interactor
- MCMVMaize chlorotic mottle viruse3pathogen
- Gene
- ZmECT18 and ZmGST31e3
- Outcome
- MCMV infectione3
- Direction
- requirede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Moreover, the results of virus-induced gene silencing (VIGS) assays showed that ZmECT18 and ZmGST31 were required for MCMV infection, while silencing of ZmMTC, ZmSCI1 or ZmTIP1 significantly promoted MCMV infection in maize”
Claim · model-written summary
Silencing of ZmMTC, ZmSCI1 or ZmTIP1 significantly promoted MCMV infection in maize.
- Treatment
- silencing of ZmMTC, ZmSCI1 or ZmTIP1e3
- Interactor
- MCMVMaize chlorotic mottle viruse3pathogen
- Gene
- ZmMTC, ZmSCI1 or ZmTIP1e3
- Outcome
- MCMV infectione3
- Direction
- promotede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Moreover, the results of virus-induced gene silencing (VIGS) assays showed that ZmECT18 and ZmGST31 were required for MCMV infection, while silencing of ZmMTC, ZmSCI1 or ZmTIP1 significantly promoted MCMV infection in maize”
The same questions in other papers
loss of function · Maize chlorotic mottle virus · Zea mays (maize)Results agree
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- negative
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