Abstract
BACKGROUND: Salinity is one of the most widespread agricultural problems in arid and semi-arid regions that makes fields unproductive, and soil salinization is a serious problem in the entire world. To determine the effects of salt stress on soybean seedlings, a proteomic technique was used. RESULTS: Soybean plants were exposed to 0, 20, 40, or 80 mM NaCl for one week. The effect of treatment at 20 mM NaCl on plant growth was not severe, at 80 mM NaCl was lethal, and at 40 mM NaCl was significant but not lethal. Based on these results, proteins were extracted from the leaves, hypocotyls and roots of soybean treated with 40 mM NaCl. Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment. In leaves, hypocotyls and roots, metabolism related proteins were mainly down-regulated with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was down-regulated in the leaf/hypocotyls, and fructokinase 2 was down-regulated in the hypocotyls/root with NaCl treatment. Stem 31 kDa glycoprotein precursor was up-regulated in all three organs with NaCl treatment. Glyceraldehyde-3-phosphate dehydrogenase was specifically down-regulated at the RNA and protein levels by salt stress. CONCLUSION: These results suggest that metabolism related proteins play a role in each organ in the adaptation to saline conditions.
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Claims 11
Claim · model-written summary
At 20 mM NaCl, the growth effect was not severe.
- Taxon
- soybean
Glycine maxt0 - Treatment
- 20 mM NaCle0
- Outcome
- plant growthe0
- Direction
- not severee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effect of treatment at 20 mM NaCl on plant growth was not severe”
t0“soybean leaves”
Claim · model-written summary
At 80 mM NaCl, the effect on soybean plants was lethal.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 80 mM NaCle0
- Outcome
- lethale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at 80 mM NaCl was lethal”
t0“soybean leaves”
Claim · model-written summary
At 40 mM NaCl, soybean growth was significantly affected but plants survived.
- Taxon
- soybeanGlycine maxt0
- Treatment
- 40 mM NaCle0
- Outcome
- significant but not lethale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at 40 mM NaCl was significant but not lethal”
t0“soybean leaves”
Claim · model-written summary
Salt treatment regulated 19 of 340 leaf proteins.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- leavese0
- Outcome
- proteins in the leavese0
- Direction
- up- and down-regulatede0
- Value
- Nineteene0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment”
t0“soybean leaves”
Claim · model-written summary
Salt treatment regulated 22 of 330 hypocotyl proteins.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- hypocotylse0
- Outcome
- proteins in the leaves, hypocotyls and rootse0
- Direction
- up- and down-regulatede0
- Value
- 22e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment”
t0“soybean leaves”
Claim · model-written summary
Salt treatment regulated 14 of 235 root proteins.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- rootse0
- Outcome
- proteins in the leaves, hypocotyls and rootse0
- Direction
- up- and down-regulatede0
- Value
- 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nineteen, 22 and 14 proteins out of 340, 330 and 235 proteins in the leaves, hypocotyls and roots, respectively, were up- and down-regulated by NaCl treatment”
t0“soybean leaves”
Claim · model-written summary
Metabolism-related proteins were mainly down-regulated by salt in leaves, hypocotyls and roots.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- leaves, hypocotyls and rootse0
- Treatment
- NaCl treatmente0
- Outcome
- metabolism related proteinse0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In leaves, hypocotyls and roots, metabolism related proteins were mainly down-regulated with NaCl treatment”
t0“soybean leaves”
Claim · model-written summary
Salt down-regulated glyceraldehyde-3-phosphate dehydrogenase in leaves and hypocotyls.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- leaf/hypocotylse0
- Gene
- Glyceraldehyde-3-phosphate dehydrogenasee0
- Outcome
- Glyceraldehyde-3-phosphate dehydrogenasee0
- Direction
- down-regulatede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glyceraldehyde-3-phosphate dehydrogenase was down-regulated in the leaf/hypocotyls”
t0“soybean leaves”
Claim · model-written summary
Salt down-regulated fructokinase 2 in hypocotyls and roots.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- hypocotyls/roote0
- Treatment
- NaCl treatmente0
- Gene
- fructokinase 2e0
- Outcome
- fructokinase 2e0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fructokinase 2 was down-regulated in the hypocotyls/root with NaCl treatment”
t0“soybean leaves”
Claim · model-written summary
Salt up-regulated stem 31 kDa glycoprotein precursor in all three organs.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- all three organse0
- Treatment
- NaCl treatmente0
- Gene
- Stem 31 kDa glycoprotein precursore0
- Outcome
- Stem 31 kDa glycoprotein precursore0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stem 31 kDa glycoprotein precursor was up-regulated in all three organs with NaCl treatment”
t0“soybean leaves”
Claim · model-written summary
Salt specifically down-regulated glyceraldehyde-3-phosphate dehydrogenase at RNA and protein levels.
- Taxon
- soybeanGlycine maxt0
- Treatment
- salt stresse0
- Gene
- Glyceraldehyde-3-phosphate dehydrogenasee0
- Outcome
- RNA and protein levelse0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glyceraldehyde-3-phosphate dehydrogenase was specifically down-regulated at the RNA and protein levels by salt stress”
t0“soybean leaves”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.