Abstract
Somatic embryos were induced directly on immature embryos of Carica papaya × Carica cauliflora hybrids cultured on modified Murashige and Skoog's medium. When transferred to medium supplemented with abscisic acid, individual somatic embryos proliferated numerous daughter embryos through repeated embryogenesis. Light microscopic study of the repeatedly embryogenic cultures showed that daughter embryos arose from single superficial cells of parent embryos. Plant regeneration occurred following transfer of somatic embryos to medium devoid of plant growth regulators. Regenerated plants were intermediate between C. papaya and C. cauliflora in several morphological respects and showed isozyme patterns specific to both species as well as some new bands, indicating that they are indeed interspecific hybrids. Key words: Carica, interspecific hybrid, embryo culture, somatic embryogenesis.
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Claims 6
Claim · model-written summary
Somatic embryos formed directly on immature Carica hybrid embryos in modified Murashige and Skoog medium.
- Taxon
- Carica papaya × Carica cauliflora
_-_DPLA_-_55200ec009622d7d42349f3583df310e.jpg?width=60)
Carica papayae0 - Material
- hybridse0
- Organ / stage
- immature embryose0
- Outcome
- Somatic embryos were induced directlye0
- Setting
- modified Murashige and Skoog's mediume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_-_DPLA_-_55200ec009622d7d42349f3583df310e.jpg?width=500)
Verbatim
e0“Somatic embryos were induced directly on immature embryos of Carica papaya × Carica cauliflora hybrids cultured on modified Murashige and Skoog's medium.”
Claim · model-written summary
Abscisic acid medium induced repeated daughter embryo proliferation.
- Taxon
- Carica papaya × Carica caulifloraCarica papayat0
- Treatment
- medium supplemented with abscisic acide0
- Outcome
- proliferated numerous daughter embryose0
- Direction
- proliferatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carica papaya × Carica cauliflora”
e0“When transferred to medium supplemented with abscisic acid, individual somatic embryos proliferated numerous daughter embryos through repeated embryogenesis.”
Claim · model-written summary
Daughter embryos arose from single superficial cells of parent embryos.
- Taxon
- Carica papaya × Carica caulifloraCarica papayat0
- Outcome
- daughter embryos arose from single superficial cells of parent embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carica papaya × Carica cauliflora”
e0“daughter embryos arose from single superficial cells of parent embryos”
Claim · model-written summary
Somatic embryos regenerated plants after transfer to regulator-free medium.
- Taxon
- Carica papaya × Carica caulifloraCarica papayat0
- Treatment
- medium devoid of plant growth regulatorse0
- Outcome
- Plant regeneration occurrede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carica papaya × Carica cauliflora”
e0“Plant regeneration occurred following transfer of somatic embryos to medium devoid of plant growth regulators.”
Claim · model-written summary
Regenerated plants had morphology intermediate between the parental species.
- Taxon
- Carica papaya × Carica caulifloraCarica papayat0
- Outcome
- intermediate between C. papaya and C. cauliflora in several morphological respectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carica papaya × Carica cauliflora”
e0“Regenerated plants were intermediate between C. papaya and C. cauliflora in several morphological respects”
Claim · model-written summary
Regenerated plants showed isozyme patterns from both parents and new bands.
- Taxon
- Carica papaya × Carica caulifloraCarica papayat0
- Outcome
- isozyme patterns specific to both species as well as some new bandse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Carica papaya × Carica cauliflora”
e0“showed isozyme patterns specific to both species as well as some new bands”
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