Abstract
The study on the chemical constituents of an essential oil of Pogostemon cablin was carried out by hydrodistillation of leaf explants and the oil analysed by Gas Chromatography Mass Spectrometry (GC/MS). The oil yield was found to be 0.30 % (v/w) of fresh weight. Twenty two compounds were identified by GC/ MS as eighteen sesquiterpenes and three oxygenated sesquiterpenes. Among these, patchouli alcohol (60.30 %) was the major component, followed by germacrene A (11.73 %). In order to study the chemical constituents of the essential oil of plant cell cultures, leaves were surface sterilised and callus cultures initiated on MS media containing naphthaleneacetic acid (0.5 mg/l), and benzyladenine (1 mg/l), followed by incubation in suitable culture conditions. Cell suspension cultures were initiated by subculturing callus cultures into new liquid media and maintained in the same conditions. Chemical constituents of the essential oils produced by both callus and cell suspension cultures were extracted with dichloromethane and analysed by GC and GC/ MS. The results showed that essential oil obtained from these cultures contained the same major constituents, namely patchouli alcohol, as in the intact plant, but the level was low, and also contained a small amount of minor constituents. Feeding cis-farnesol, the precursor of patchouli alcohol, to cell suspension cultures resulted in the patchouli alcohol being increased from 19.5 mg/l to 25.5 mg/l.
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Claims 7
Claim · model-written summary
Leaf essential oil yield was 0.30% of fresh weight.
- Taxon
- Pogostemon cablin
Pogostemon cabline1 - Organ / stage
- leaf explantse1
- Outcome
- oil yielde0
- Value
- 0.30 % (v/w)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The oil yield was found to be 0.30 % (v/w) of fresh weight.”
e1“essential oil of Pogostemon cablin was carried out by hydrodistillation of leaf explants”
Claim · model-written summary
The leaf oil contained twenty two identified compounds, described as eighteen sesquiterpenes and three oxygenated sesquiterpenes.
- Taxon
- Pogostemon cablinPogostemon cabline1
- Outcome
- compoundse0
- Value
- Twenty twoe0
- Qualifier
- eighteen sesquiterpenes and three oxygenated sesquiterpenese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty two compounds were identified by GC/ MS as eighteen sesquiterpenes and three oxygenated sesquiterpenes.”
e1“essential oil of Pogostemon cablin”
Claim · model-written summary
Patchouli alcohol was the major component of leaf oil at 60.30%.
- Taxon
- Pogostemon cablinPogostemon cabline1
- Outcome
- patchouli alcohole0
- Direction
- majore0
- Value
- 60.30 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“patchouli alcohol (60.30 %) was the major component”
e1“essential oil of Pogostemon cablin”
Claim · model-written summary
Germacrene A followed patchouli alcohol at 11.73%.
- Taxon
- Pogostemon cablinPogostemon cabline1
- Outcome
- germacrene Ae0
- Value
- 11.73 %e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“followed by germacrene A (11.73 %)”
e1“essential oil of Pogostemon cablin”
Claim · model-written summary
Callus and suspension culture oils contained patchouli alcohol at a low level relative to intact plants.
- Taxon
- Pogostemon cablinPogostemon cabline1
- Outcome
- patchouli alcohole0
- Direction
- lowe0
- Comparator
- intact plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“essential oil obtained from these cultures contained the same major constituents, namely patchouli alcohol, as in the intact plant, but the level was low”
e1“essential oil of Pogostemon cablin”
Claim · model-written summary
Cultured cell oils contained a small amount of minor constituents.
- Taxon
- Pogostemon cablinPogostemon cabline1
- Outcome
- minor constituentse0
- Qualifier
- small amounte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“also contained a small amount of minor constituents”
e1“essential oil of Pogostemon cablin”
Claim · model-written summary
Cis-farnesol feeding increased patchouli alcohol in cell suspension cultures from 19.5 mg/l to 25.5 mg/l.
- Taxon
- Pogostemon cablinPogostemon cabline1
- Organ / stage
- cell suspension culturese0
- Treatment
- Feeding cis-farnesole0
- Outcome
- patchouli alcohole0
- Direction
- increasede0
- Value
- 25.5 mg/le0
- Comparator
- 19.5 mg/le0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Feeding cis-farnesol, the precursor of patchouli alcohol, to cell suspension cultures resulted in the patchouli alcohol being increased from 19.5 mg/l to 25.5 mg/l.”
e1“essential oil of Pogostemon cablin”
The same questions in other papers
infection · molecular: metabolites · Pogostemon cablin (patchouli)Positive and negative results
1 in the opposite direction
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.