Paper · 1997 · Annals of Applied Biology

Long term water stress inactivates Rubisco in subterranean clover

H. MEDRANO; M A J PARRY; X. SOCIAS; et al.

7
claims
7
verbatim quotes
122
citations
1
research areas
—
Not open access

Abstract

Summary In long‐term field experiments, during consecutive years, microswards of subterranean clover were irrigated to minimise water deficits or subjected to progressively increasing drought over 30 days. Both leaf water potential and relative water content steadily decreased during the experiments. Plants affected by drought grew more slowly and photosynthesis was decreased. Photosynthetic rate (A) and Rubisco were analysed in relation to midday water potentials and relative water contents. The difference in A between draughted and irrigated plants increased progressively, in part as a result of decreased stomatal conductance and CO 2 concentration within leaf (Ci). However, A‐Ci curves suggest that the photosynthetic capacity in plants experiencing long‐term stress was reduced by 50% when compared with irrigated plants. Drought decreased both the initial and the total Rubisco activity per unit area in a similar way but did not reduce the amount of Rubisco protein per unit leaf area. Thus, the specific activity of Rubisco, rather than its activation state, decreased suggesting that under water stress the active sites were blocked by inhibitors.

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Claims 7

Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Leaf water potential and relative water content decreased during the drought experiments.

Outcome
leaf water potential and relative water contente0
Direction
decreasede0

Not reportedTaxon

Verbatim

e0

“Both leaf water potential and relative water content steadily decreased during the experiments.”

✓ located verbatim in the source · abstract, characters 206–301 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Drought affected plants grew more slowly and had decreased photosynthesis.

Taxon
PlantsViridiplantaee0
Treatment
droughte0
Outcome
grew more slowly and photosynthesise0
Direction
decreasede0

Not reportedComparator

Verbatim

e0

“Plants affected by drought grew more slowly and photosynthesis was decreased.”

✓ located verbatim in the source · abstract, characters 302–379 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The photosynthetic difference between droughted and irrigated plants progressively increased.

Taxon
plantsViridiplantaee0
Treatment
draughtede0
Outcome
difference in Ae0
Direction
increased progressivelye0
Comparator
irrigated plantse0

Verbatim

e0

“The difference in A between draughted and irrigated plants increased progressively, in part as a result of decreased stomatal conductance and CO 2 concentration within leaf (Ci).”

✓ located verbatim in the source · abstract, characters 498–676 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Long-term stressed plants had 50% lower photosynthetic capacity than irrigated plants.

Taxon
plantsViridiplantaee0
Treatment
long‐term stresse0
Outcome
photosynthetic capacitye0
Direction
reducede0
Value
50%e0
Comparator
irrigated plantse0

Verbatim

e0

“photosynthetic capacity in plants experiencing long‐term stress was reduced by 50% when compared with irrigated plants.”

✓ located verbatim in the source · abstract, characters 715–834 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Drought decreased initial and total Rubisco activity per unit area.

Treatment
Droughte0
Gene
Rubiscoe0
Outcome
initial and the total Rubisco activity per unit areae0
Direction
decreasede0

Not reportedTaxonComparator

Verbatim

e0

“Drought decreased both the initial and the total Rubisco activity per unit area in a similar way but did not reduce the amount of Rubisco protein per unit leaf area.”

✓ located verbatim in the source · abstract, characters 835–1000 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Drought did not reduce Rubisco protein per unit leaf area.

Treatment
Droughte0
Gene
Rubiscoe0
Outcome
amount of Rubisco protein per unit leaf areae0
Direction
did not reducee0

Not reportedTaxonComparator

Verbatim

e0

“Drought decreased both the initial and the total Rubisco activity per unit area in a similar way but did not reduce the amount of Rubisco protein per unit leaf area.”

✓ located verbatim in the source · abstract, characters 835–1000 · show in text ↑
Observationauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Rubisco specific activity decreased under water stress, suggesting inhibitor blockage of active sites.

Gene
Rubiscoe0
Outcome
specific activity of Rubiscoe0
Direction
decreasede0
Qualifier
under water stress the active sites were blocked by inhibitorse0

Not reportedTaxon

Verbatim

e0

“the specific activity of Rubisco, rather than its activation state, decreased suggesting that under water stress the active sites were blocked by inhibitors.”

✓ located verbatim in the source · abstract, characters 1007–1164 · show in text ↑

drought · physiology: photosynthesis · Viridiplantae (plants)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. negative
  3. negative
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.