Abstract
A highly sensitive quantitative method for assaying nicotianamine (NA) and 2'-deoxymugineic acid (DMA) using liquid chromatography electrospray ionization time-of-flight mass spectrometry (LC-ESI-TOF-MS) was developed. This method requires fewer sample preparation steps than high-performance liquid chromatography (HPLC). The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride, which improved their retention on a C18 reversed-phase column and facilitated separation. The amounts of NA and DMA in 10 μl of xylem sap from rice cultivated under Fe-sufficient and Fe-deficient conditions were quantified without concentration. In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe. In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly.
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Claims 7
Claim · model-written summary
The developed assay required fewer preparation steps than HPLC.
- Taxon
- rice
Oryza sativae2 - Treatment
- liquid chromatography electrospray ionization time-of-flight mass spectrometry (LC-ESI-TOF-MS)e1
- Outcome
- sample preparation stepse0
- Direction
- fewere0
- Comparator
- high-performance liquid chromatography (HPLC)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method requires fewer sample preparation steps than high-performance liquid chromatography (HPLC).”
e1“using liquid chromatography electrospray ionization time-of-flight mass spectrometry (LC-ESI-TOF-MS)”
e2“xylem sap from rice”
Claim · model-written summary
Derivatization improved retention of NA and DMA on a C18 column.
- Taxon
- riceOryza sativae1
- Treatment
- derivatized using 9-fluorenylmethoxycarbonyl chloridee0
- Outcome
- retention on a C18 reversed-phase columne0
- Direction
- improvede0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride, which improved their retention on a C18 reversed-phase column and facilitated separation.”
e1“xylem sap from rice”
Claim · model-written summary
Derivatization facilitated separation of NA and DMA.
- Taxon
- riceOryza sativae1
- Treatment
- derivatized using 9-fluorenylmethoxycarbonyl chloridee0
- Outcome
- separatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amino and hydroxyl groups of NA and DMA were derivatized using 9-fluorenylmethoxycarbonyl chloride, which improved their retention on a C18 reversed-phase column and facilitated separation.”
e1“xylem sap from rice”
Claim · model-written summary
The assay quantified NA and DMA in 10 μl of rice xylem sap without concentration.
- Taxon
- riceOryza sativae0
- Organ / stage
- xylem sape0
- Outcome
- amounts of NA and DMAe0
- Value
- 10 μle0
- Qualifier
- without concentratione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The amounts of NA and DMA in 10 μl of xylem sap from rice cultivated under Fe-sufficient and Fe-deficient conditions were quantified without concentration.”
Claim · model-written summary
In Fe-sufficient rice, NA and DMA concentrations were nearly equal to Fe concentration.
- Taxon
- riceOryza sativae1
- Outcome
- concentrations of NA and DMAe0
- Direction
- almost equale0
- Qualifier
- Fe-sufficient plantse0
- Comparator
- Fee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Fe-sufficient plants, the concentrations of NA and DMA were almost equal to that of Fe.”
e1“xylem sap from rice”
Claim · model-written summary
NA concentration did not change significantly in Fe-deficient rice.
- Taxon
- riceOryza sativae1
- Treatment
- Fe-deficiente0
- Outcome
- concentration of NAe0
- Direction
- did not changee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Fe-deficient plants, the concentration of NA did not change significantly”
e1“xylem sap from rice”
Claim · model-written summary
DMA concentration increased markedly in Fe-deficient rice.
- Taxon
- riceOryza sativae1
- Treatment
- Fe-deficiente0
- Outcome
- DMAe0
- Direction
- increased markedlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Fe-deficient plants, the concentration of NA did not change significantly, whereas that of DMA increased markedly.”
e1“xylem sap from rice”