Abstract
Many plant viruses encode proteins that suppress RNA silencing, but little is known about the activity of silencing suppressors in roots. This study examined differences in the silencing suppression activity of different viruses in leaves and roots of Nicotiana benthamiana plants. Infection by tobacco mosaic virus, potato virus Y and cucumber mosaic virus but not potato virus X (PVX) resulted in strong silencing suppression activity of a transgene in both leaves and roots, whereas infection by beet necrotic yellow vein virus (BNYVV) and tobacco rattle virus (TRV) showed transgene silencing suppression in roots but not in leaves. For most viruses tested, viral negative-strand RNA accumulated at a very low level in roots, compared with considerable levels of positive-strand genomic RNA. Co-inoculation of leaves with PVX and either BNYVV or TRV produced an increase in PVX negative-strand RNA and subgenomic RNA (sgRNA) accumulation in roots. The cysteine-rich proteins (CRPs) BNYVV p14 and TRV 16K showed weak silencing suppression activity in leaves. However, when either of these CRPs was expressed from a PVX vector, there was an enhancement of PVX negative-strand RNA and sgRNA accumulation in roots compared with PVX alone. Such enhancement of PVX sgRNAs was also observed by expression of CRPs of other viruses and the well-known suppressors HC-Pro and p19 but not of the potato mop-top virus p8 CRP. These results indicate that BNYVV- and TRV-encoded CRPs suppress RNA silencing more efficiently in roots than in leaves.
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Claims 13
Claim · model-written summary
Tobacco mosaic virus infection strongly suppressed transgene silencing in leaves and roots.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Outcome
- strong silencing suppression activity of a transgene in both leaves and rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection by tobacco mosaic virus, potato virus Y and cucumber mosaic virus but not potato virus X (PVX) resulted in strong silencing suppression activity of a transgene in both leaves and roots”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
Potato virus Y infection strongly suppressed transgene silencing in leaves and roots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- potato virus Y
Potato virus Ye0pathogen - Outcome
- strong silencing suppression activity of a transgene in both leaves and rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection by tobacco mosaic virus, potato virus Y and cucumber mosaic virus but not potato virus X (PVX) resulted in strong silencing suppression activity of a transgene in both leaves and roots”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
Cucumber mosaic virus infection strongly suppressed transgene silencing in leaves and roots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- cucumber mosaic virus
cucumber mosaic cucumoviruse0pathogen - Outcome
- strong silencing suppression activity of a transgene in both leaves and rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection by tobacco mosaic virus, potato virus Y and cucumber mosaic virus but not potato virus X (PVX) resulted in strong silencing suppression activity of a transgene in both leaves and roots”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
Potato virus X infection did not strongly suppress transgene silencing in leaves and roots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- potato virus XPotato virus Xe0pathogen
- Outcome
- silencing suppression activity of a transgene in both leaves and rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not potato virus X (PVX) resulted in strong silencing suppression activity of a transgene in both leaves and roots”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
Beet necrotic yellow vein virus infection suppressed transgene silencing in roots but not leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- beet necrotic yellow vein virus
Beet necrotic yellow vein viruse0pathogen - Outcome
- transgene silencing suppression in roots but not in leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infection by beet necrotic yellow vein virus (BNYVV) and tobacco rattle virus (TRV) showed transgene silencing suppression in roots but not in leaves”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
Tobacco rattle virus infection suppressed transgene silencing in roots but not leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- tobacco rattle virusTobacco rattle viruse0pathogen
- Outcome
- transgene silencing suppression in roots but not in leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infection by beet necrotic yellow vein virus (BNYVV) and tobacco rattle virus (TRV) showed transgene silencing suppression in roots but not in leaves”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
For most tested viruses, negative-strand RNA accumulated at very low levels in roots compared with positive-strand genomic RNA.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Outcome
- viral negative-strand RNAe0
- Direction
- very low levele0
- Comparator
- positive-strand genomic RNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For most viruses tested, viral negative-strand RNA accumulated at a very low level in roots, compared with considerable levels of positive-strand genomic RNA”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
Co-inoculation with PVX and BNYVV increased PVX negative-strand RNA and subgenomic RNA in roots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- Co-inoculation of leaves with PVX and either BNYVV or TRVe0
- Outcome
- PVX negative-strand RNA and subgenomic RNA (sgRNA) accumulation in rootse0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-inoculation of leaves with PVX and either BNYVV or TRV produced an increase in PVX negative-strand RNA and subgenomic RNA (sgRNA) accumulation in roots”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
BNYVV p14 showed weak silencing suppression activity in leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- BNYVV p14e0
- Outcome
- weak silencing suppression activity in leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cysteine-rich proteins (CRPs) BNYVV p14 and TRV 16K showed weak silencing suppression activity in leaves”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
TRV 16K showed weak silencing suppression activity in leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- TRV 16Ke0
- Outcome
- weak silencing suppression activity in leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cysteine-rich proteins (CRPs) BNYVV p14 and TRV 16K showed weak silencing suppression activity in leaves”
e1“Nicotiana benthamiana plants”
Claim · model-written summary
Expressing BNYVV p14 from a PVX vector enhanced PVX negative-strand and subgenomic RNA in roots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae2
- Treatment
- expressed from a PVX vectore0
- Gene
- BNYVV p14e1
- Outcome
- PVX negative-strand RNA and sgRNA accumulation in rootse0
- Direction
- enhancemente0
- Comparator
- PVX alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when either of these CRPs was expressed from a PVX vector, there was an enhancement of PVX negative-strand RNA and sgRNA accumulation in roots compared with PVX alone”
e1“BNYVV p14 and TRV 16K”
e2“Nicotiana benthamiana plants”
Claim · model-written summary
Expressing TRV 16K from a PVX vector enhanced PVX negative-strand and subgenomic RNA in roots.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae2
- Treatment
- expressed from a PVX vectore0
- Gene
- TRV 16Ke1
- Outcome
- PVX negative-strand RNA and sgRNA accumulation in rootse0
- Direction
- enhancemente0
- Comparator
- PVX alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when either of these CRPs was expressed from a PVX vector, there was an enhancement of PVX negative-strand RNA and sgRNA accumulation in roots compared with PVX alone”
e1“BNYVV p14 and TRV 16K”
e2“Nicotiana benthamiana plants”
Claim · model-written summary
Other viral cysteine-rich proteins and HC-Pro and p19 enhanced PVX subgenomic RNA, while potato mop-top virus p8 did not.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Gene
- HC-Pro and p19e0
- Outcome
- enhancement of PVX sgRNAse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such enhancement of PVX sgRNAs was also observed by expression of CRPs of other viruses and the well-known suppressors HC-Pro and p19 but not of the potato mop-top virus p8 CRP”
e1“Nicotiana benthamiana plants”
The same questions in other papers
Nicotiana tabacum · molecular: metabolites · Nicotiana benthamianaPositive and negative results
2 in the opposite direction
- positive
- positive
Solanum tuberosum · molecular: metabolites · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- negative
inoculation · molecular: metabolites · Nicotiana benthamianaPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.