Abstract
Similarly to other potyvirids, the bymovirus wheat yellow mosaic virus (WYMV) encodes a P3N-PIPO protein that is expressed by frameshifting occurring within the open reading frame of the P3 protein. P3N-PIPO is known to be essential for the cell-to-cell movement of several potyviruses, but this has not yet been confirmed for the WYMV. Here, we show that the WYMV P3N-PIPO protein influences disease symptom formation. Infection of Nicotiana benthamiana plants with a potato virus X (PVX)-based vector carrying the WYMV P3N-PIPO gene induced more severe disease symptoms and resulted in higher virus accumulation levels than did infection with PVX lacking the P3N-PIPO gene. N. benthamiana P3N-PIPO-interacting proteins were identified through co-immunoprecipitation (Co-IP) coupled with LC-MS/MS (mass spectrometry), and the interaction between P3N-PIPO and the N. benthamiana receptor-like kinase NbRLK6 was further verified by Co-IP and bimolecular fluorescence complementation (BiFC) of transiently-expressed proteins. Furthermore, our investigation showed that the disease symptom severity and accumulation level of PVX-P3N-PIPO were decreased in N. benthamiana plants when NbRLK6 expression was reduced by tobacco rattle virus-induced gene silencing.
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Claims 6
Claim · model-written summary
WYMV P3N-PIPO in a PVX vector caused more severe disease symptoms than PVX without the gene.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat0 - Treatment
- PVX)-based vector carrying the WYMV P3N-PIPO genee0
- Interactor
- potato virus XPotato virus Xe0pathogen
- Gene
- WYMV P3N-PIPO genee0
- Outcome
- disease symptomse0
- Direction
- more severee0
- Comparator
- PVX lacking the P3N-PIPO genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection of Nicotiana benthamiana plants with a potato virus X (PVX)-based vector carrying the WYMV P3N-PIPO gene induced more severe disease symptoms and resulted in higher virus accumulation levels than did infection with PVX lacking the P3N-PIPO gene”
t0“Nicotiana benthamiana”
Claim · model-written summary
WYMV P3N-PIPO in a PVX vector increased viral accumulation relative to PVX without the gene.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- PVX)-based vector carrying the WYMV P3N-PIPO genee0
- Interactor
- potato virus XPotato virus Xe0pathogen
- Gene
- WYMV P3N-PIPO genee0
- Outcome
- virus accumulation levelse0
- Direction
- highere0
- Comparator
- PVX lacking the P3N-PIPO genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Infection of Nicotiana benthamiana plants with a potato virus X (PVX)-based vector carrying the WYMV P3N-PIPO gene induced more severe disease symptoms and resulted in higher virus accumulation levels than did infection with PVX lacking the P3N-PIPO gene”
t0“Nicotiana benthamiana”
Claim · model-written summary
P3N-PIPO-interacting host proteins were identified.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- P3N-PIPOe0
- Outcome
- P3N-PIPO-interacting proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“N. benthamiana P3N-PIPO-interacting proteins were identified through co-immunoprecipitation (Co-IP) coupled with LC-MS/MS”
t0“Nicotiana benthamiana”
Claim · model-written summary
P3N-PIPO interaction with NbRLK6 was verified.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Gene
- NbRLK6e0
- Outcome
- interaction between P3N-PIPO and the N. benthamiana receptor-like kinase NbRLK6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the interaction between P3N-PIPO and the N. benthamiana receptor-like kinase NbRLK6 was further verified by Co-IP and bimolecular fluorescence complementation (BiFC)”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbRLK6 reduced PVX-P3N-PIPO symptom severity.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbRLK6 expression was reduced by tobacco rattle virus-induced gene silencinge0
- Interactor
- PVX-P3N-PIPOe0pathogen
- Gene
- NbRLK6e0
- Outcome
- disease symptom severitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disease symptom severity and accumulation level of PVX-P3N-PIPO were decreased in N. benthamiana plants when NbRLK6 expression was reduced by tobacco rattle virus-induced gene silencing”
t0“Nicotiana benthamiana”
Claim · model-written summary
Silencing NbRLK6 reduced PVX-P3N-PIPO accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- NbRLK6 expression was reduced by tobacco rattle virus-induced gene silencinge0
- Interactor
- PVX-P3N-PIPOe0pathogen
- Gene
- NbRLK6e0
- Outcome
- accumulation level of PVX-P3N-PIPOe0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disease symptom severity and accumulation level of PVX-P3N-PIPO were decreased in N. benthamiana plants when NbRLK6 expression was reduced by tobacco rattle virus-induced gene silencing”
t0“Nicotiana benthamiana”
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