Abstract
Monopartite begomoviruses and their associated satellites form unique disease complexes that have emerged as a serious threat to agriculture worldwide. It is well known that frequent recombination contributes to the diversification and evolution of geminiviruses. In this study, we identified a novel defective satellite molecule (RecSat) in association with Tobacco leaf curl Yunnan virus (TbLCYNV) in a naturally infected tobacco plant. Sequence analysis showed that Recsat comprises 754 nucleotides in size and is a chimera involving alphasatellite and betasatellite sequences, containing both betasatellite-conserved region and alphasatellite stem-loop structure. Recombination analysis revealed that RecSat has arisen from three independent recombination events likely involving Tomato yellow leaf curl China betasatellite, Ageratum yellow vein China betasatellite and Tobacco curly shoot alphasatellite. Co-inoculation of RecSat with TbLCYNV induced symptoms indistinguishable from those induced by TbLCYNV alone in Nicotiana benthamiana. Southern blot hybridization showed that RecSat could be trans-replicated stably in N. benthamiana plants by TbLCYNV, and impaired the accumulation of helper virus and co-inoculated alphasatellite. Our results provide the first evidence for recombination between two distinct types of satellites among geminivirus complex and highlight recombination as a driving force for geminivirus evolution.
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Claims 6
Claim · model-written summary
A novel defective RecSat satellite was found with Tobacco leaf curl Yunnan virus in infected tobacco.
- Taxon
- tobacco plant
Nicotiana tabacume0 - Interactor
- Tobacco leaf curl Yunnan virus (TbLCYNV)Tobacco leaf curl Yunnan viruse0pathogen
- Outcome
- novel defective satellite molecule (RecSat)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified a novel defective satellite molecule (RecSat) in association with Tobacco leaf curl Yunnan virus (TbLCYNV) in a naturally infected tobacco plant.”
Claim · model-written summary
RecSat was 754 nucleotides long and combined alpha- and betasatellite sequences and structures.
- Taxon
- tobacco plantNicotiana tabacume1
- Outcome
- chimera involving alphasatellite and betasatellite sequencese0
- Value
- 754 nucleotidese0
- Qualifier
- betasatellite-conserved region and alphasatellite stem-loop structuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Recsat comprises 754 nucleotides in size and is a chimera involving alphasatellite and betasatellite sequences, containing both betasatellite-conserved region and alphasatellite stem-loop structure.”
t0“in a naturally infected tobacco plant”
e1“in a naturally infected tobacco plant”
Claim · model-written summary
RecSat likely arose through three independent recombination events involving three named satellites.
- Taxon
- tobacco plantNicotiana tabacume1
- Outcome
- RecSat has arisene0
- Value
- three independent recombination eventse0
- Qualifier
- likely involving Tomato yellow leaf curl China betasatellite, Ageratum yellow vein China betasatellite and Tobacco curly shoot alphasatellitee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RecSat has arisen from three independent recombination events likely involving Tomato yellow leaf curl China betasatellite, Ageratum yellow vein China betasatellite and Tobacco curly shoot alphasatellite.”
t0“in a naturally infected tobacco plant”
e1“in a naturally infected tobacco plant”
Claim · model-written summary
RecSat co-inoculation with TbLCYNV gave symptoms indistinguishable from virus alone.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Co-inoculation of RecSat with TbLCYNVe0
- Interactor
- TbLCYNVe0pathogen
- Outcome
- symptomse0
- Direction
- indistinguishablee0
- Comparator
- TbLCYNV alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-inoculation of RecSat with TbLCYNV induced symptoms indistinguishable from those induced by TbLCYNV alone in Nicotiana benthamiana.”
Claim · model-written summary
TbLCYNV stably trans-replicated RecSat in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- TbLCYNVe0pathogen
- Outcome
- RecSat could be trans-replicated stablye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RecSat could be trans-replicated stably in N. benthamiana plants by TbLCYNV”
Claim · model-written summary
RecSat impaired accumulation of helper virus and co-inoculated alphasatellite.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- RecSate0
- Interactor
- TbLCYNVe0pathogen
- Outcome
- accumulation of helper virus and co-inoculated alphasatellitee0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RecSat could be trans-replicated stably in N. benthamiana plants by TbLCYNV, and impaired the accumulation of helper virus and co-inoculated alphasatellite.”
The same questions in other papers
Nicotiana tabacum · disease · Nicotiana benthamianaPositive and negative results
2 papers in the same direction as this paper · 1 reporting no difference from an alternative
- positive
- negative
- negative
Yunnan orbivirus · disease · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- negative
inoculation · disease · Nicotiana benthamianaPositive and negative results
31 papers in the same direction as this paper · 8 reporting no difference from an alternative
- positive
- positive
- positive
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.