Abstract
In vitro regeneration of two cultivars of carnation, namely, ‘Nelson’ and ‘Impulse’ was studied through direct somatic embryogenesis. Somatic embryos were formed directly on petal explants cultured on Murashige and Skoog (MS) medium containing different concentrations (0.2, 0.5, 1, 2, 4, 6 mg 1-1) of picloram. Maximum embryogenesis was obtained with 1 and 2 mg/l picloram. Globular shaped embryos were developed into cotyledonary-shaped embryos when they were transferred to the growth regulator-free media containing different concentrations (2, 4, 6, 8, and 10 %) of sucrose. Increasing sucrose concentrations in the culture media enhanced somatic embryos development. Cotyledonary somatic embryos developed plantlets when they were transferred to the half-strength MS culture medium containing 3% sucrose. Plantlets also continued to grow under greenhouse conditions.
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Claims 6
Claim · model-written summary
Petal explants formed somatic embryos on picloram-containing MS medium.
- Taxon
- carnation
Dianthus caryophyllust0 - Organ / stage
- petal explantse0
- Treatment
- piclorame0
- Outcome
- Somatic embryos were formed directlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Somatic embryos were formed directly on petal explants cultured on Murashige and Skoog (MS) medium containing different concentrations (0.2, 0.5, 1, 2, 4, 6 mg 1-1) of picloram”
t0“Carnation”
Claim · model-written summary
Maximum embryogenesis occurred with 1 and 2 mg/l picloram.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- 1 and 2 mg/l piclorame0
- Outcome
- embryogenesise0
- Direction
- Maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Maximum embryogenesis was obtained with 1 and 2 mg/l picloram”
t0“Carnation”
Claim · model-written summary
Higher sucrose concentrations enhanced somatic embryo development.
- Taxon
- CarnationDianthus caryophyllust0
- Treatment
- Increasing sucrose concentrationse0
- Outcome
- somatic embryos developmente0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing sucrose concentrations in the culture media enhanced somatic embryos development”
t0“Carnation”
Claim · model-written summary
Globular embryos developed into cotyledonary embryos after transfer to sucrose-containing regulator-free medium.
- Taxon
- CarnationDianthus caryophyllust0
- Organ / stage
- Globular shaped embryose0
- Outcome
- developed into cotyledonary-shaped embryose0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Globular shaped embryos were developed into cotyledonary-shaped embryos when they were transferred to the growth regulator-free media containing different concentrations (2, 4, 6, 8, and 10 %) of sucrose”
t0“Carnation”
Claim · model-written summary
Cotyledonary somatic embryos developed plantlets on half-strength MS medium with 3% sucrose.
- Taxon
- CarnationDianthus caryophyllust0
- Organ / stage
- Cotyledonary somatic embryose0
- Outcome
- developed plantletse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotyledonary somatic embryos developed plantlets when they were transferred to the half-strength MS culture medium containing 3% sucrose”
t0“Carnation”
Claim · model-written summary
Regenerated plantlets continued growing in the greenhouse.
- Taxon
- CarnationDianthus caryophyllust0
- Outcome
- continued to growe0
- Setting
- greenhouse conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Plantlets also continued to grow under greenhouse conditions”
t0“Carnation”
The same questions in other papers
sucrose · growth · Dianthus caryophyllus (carnation)Positive and negative results
14 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.