Paper · 1983 · Physiologia Plantarum

Adventitious root formation ‘in vitro’ in apple rootstocks (Malus pumila) I. Factors affecting the length of the auxin‐sensitive phase in M.9

David J. James

7
claims
14
verbatim quotes
69
citations
3
research areas
—
Not open access

Abstract

The length of the auxin‐sensitive phase of root initiation ‘in vitro’ in the apple rootstock M.9 ( Malus pumila Mill.) has been determined using the auxins indol‐3yl‐acettc acid (IAA) at 2.8 × 10 −5 M and indol‐3yl‐butyric acid (IBA) at 1.5 × 10 −5 M in the presence and absence of 10 −3 M phloroglucinol (PG). PG synergised IBA‐induced rooting after 4 days exposure, but contact times exceeding 8 days decreased root number. In contrast, PG consistently synergised IAA‐induced rooting in the dark for contact periods up to 13 days with the highest rooting being recorded at 9 days. An irradiance of 20 W m −2 from fluorescent lamps halved IAA‐induced rooting irrespective of the presence or absence of PG. The culture of shoots at temperatures of 22,25 and 29°C during the root initiation phase (auxin present) and the root emergence phase (auxin absent) produced no difference in rooting response. In the presence of PG the use of liquid culture in place of agar‐solidified culture during the auxin‐sensitive phase reduced root number but not rooting percentage.

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Claims 7

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Phloroglucinol enhanced IBA-induced rooting after four days of exposure.

Material
M.9e1
Treatment
PGe0
Outcome
IBA‐induced rootinge0
Direction
synergisede0
Qualifier
after 4 days exposuree0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“PG synergised IBA‐induced rooting after 4 days exposure”

✓ located verbatim in the source · abstract, characters 311–366 · show in text ↑
e1

“the apple rootstock M.9 ( Malus pumila Mill.)”

✓ located verbatim in the source · abstract, characters 73–118 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Phloroglucinol contact beyond eight days reduced root number under IBA-induced rooting.

Material
M.9e1
Treatment
contact times exceeding 8 dayse0
Outcome
root numbere0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“PG synergised IBA‐induced rooting after 4 days exposure, but contact times exceeding 8 days decreased root number.”

✓ located verbatim in the source · abstract, characters 311–425 · show in text ↑
e1

“the apple rootstock M.9 ( Malus pumila Mill.)”

✓ located verbatim in the source · abstract, characters 73–118 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Phloroglucinol enhanced IAA-induced rooting in darkness for contact up to 13 days, with the highest rooting at nine days.

Material
M.9e1
Treatment
PGe0
Outcome
IAA‐induced rootinge0
Direction
synergisede0
Qualifier
in the dark for contact periods up to 13 dayse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“PG consistently synergised IAA‐induced rooting in the dark for contact periods up to 13 days with the highest rooting being recorded at 9 days.”

✓ located verbatim in the source · abstract, characters 439–582 · show in text ↑
e1

“the apple rootstock M.9 ( Malus pumila Mill.)”

✓ located verbatim in the source · abstract, characters 73–118 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Fluorescent irradiance halved IAA-induced rooting regardless of phloroglucinol.

Material
M.9e1
Treatment
20 W m −2 from fluorescent lampse0
Outcome
IAA‐induced rootinge0
Direction
halvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“An irradiance of 20 W m −2 from fluorescent lamps halved IAA‐induced rooting irrespective of the presence or absence of PG.”

✓ located verbatim in the source · abstract, characters 583–706 · show in text ↑
e1

“the apple rootstock M.9 ( Malus pumila Mill.)”

✓ located verbatim in the source · abstract, characters 73–118 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Culture at 22, 25 and 29°C produced no difference in rooting response.

Material
M.9e1
Treatment
temperatures of 22,25 and 29°Ce0
Outcome
rooting responsee0
Direction
no differencee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“The culture of shoots at temperatures of 22,25 and 29°C during the root initiation phase (auxin present) and the root emergence phase (auxin absent) produced no difference in rooting response.”

✓ located verbatim in the source · abstract, characters 707–899 · show in text ↑
e1

“the apple rootstock M.9 ( Malus pumila Mill.)”

✓ located verbatim in the source · abstract, characters 73–118 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

With phloroglucinol, liquid culture reduced root number relative to agar-solidified culture.

Material
M.9e1
Treatment
liquid culturee0
Outcome
root numbere0
Direction
reducede0
Qualifier
In the presence of PGe0
Comparator
agar‐solidified culturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“In the presence of PG the use of liquid culture in place of agar‐solidified culture during the auxin‐sensitive phase reduced root number but not rooting percentage.”

✓ located verbatim in the source · abstract, characters 900–1064 · show in text ↑
e1

“the apple rootstock M.9 ( Malus pumila Mill.)”

✓ located verbatim in the source · abstract, characters 73–118 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

With phloroglucinol, liquid culture did not reduce rooting percentage relative to agar-solidified culture.

Material
M.9e1
Treatment
liquid culturee0
Outcome
rooting percentagee0
Direction
note0
Qualifier
In the presence of PGe0
Comparator
agar‐solidified culturee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“In the presence of PG the use of liquid culture in place of agar‐solidified culture during the auxin‐sensitive phase reduced root number but not rooting percentage.”

✓ located verbatim in the source · abstract, characters 900–1064 · show in text ↑
e1

“the apple rootstock M.9 ( Malus pumila Mill.)”

✓ located verbatim in the source · abstract, characters 73–118 · show in text ↑

phloroglucinol · growth · Malus domestica (apple)Results differ

6 papers: 5 positive, 1 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. positive
  3. no effect

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.