Abstract
A cucumber mosaic virus (CMV-Ix) from Ixora is unusual in that it does not support the accumulation of some well-characterized CMV satellite RNAs in plants. CMV-Ix can support a particular satellite RNA variant which causes lethal tomato necrosis when inoculated with other CMV strains but not when inoculated with CMV-Ix. This difference in ability to support accumulation of specific satellite variants is apparent even when their sequences differ by only 10 nucleotides. Electroporation of tomato protoplasts with combinations of CMV-Ix or CMV-1 RNA plus the same satellite variants showed similar differences in accumulation, indicating a defect in satellite RNA replication and not movement or encapsidation. Pseudorecombinant virus infections between CMV-1 and CMV-Ix indicated that the genomic determinants responsible for this phenotype reside on RNA 1 since only combinations with CMV-Ix RNA 1 failed to replicate satellite RNA. The complete genome of CMV-Ix was cloned, sequenced and compared with the genomes of other cucumoviruses. CMV-Ix is most similar in RNA and protein sequence to subgroup 1 CMV-Fny and CMV-Y but slightly less similar than they are to each other. CMV-Ix and all cucumovirus strains sequenced thus far share a domain in the 3' untranslated portion of their genomic RNAs in which 39 of 40 bases are completely conserved.
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Claims 10
Claim · model-written summary
CMV-Ix did not support accumulation of some CMV satellite RNAs in plants.
- Interactor
- cucumber mosaic virus (CMV-Ix)
cucumber mosaic cucumoviruse0pathogen - Outcome
- accumulation of some well-characterized CMV satellite RNAse0
.jpg?width=60){kind=small}
IxoraOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
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Verbatim
e0“A cucumber mosaic virus (CMV-Ix) from Ixora is unusual in that it does not support the accumulation of some well-characterized CMV satellite RNAs in plants”
Claim · model-written summary
CMV-Ix supported one satellite variant.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CMV-Ix can support a particular satellite RNA variant”
t0“Ixora”
Claim · model-written summary
A satellite variant caused lethal tomato necrosis with other CMV strains but not CMV-Ix.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- CMV-Ixe0
- Interactor
- CMV-Ixe0pathogen
- Outcome
- lethal tomato necrosise0
- Direction
- note0
- Comparator
- other CMV strainse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a particular satellite RNA variant which causes lethal tomato necrosis when inoculated with other CMV strains but not when inoculated with CMV-Ix”
Claim · model-written summary
Satellite accumulation differences were evident despite only 10 nucleotide sequence differences.
- Outcome
- difference in ability to support accumulation of specific satellite variantse0
- Value
- 10 nucleotidese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This difference in ability to support accumulation of specific satellite variants is apparent even when their sequences differ by only 10 nucleotides”
t0“Ixora”
Claim · model-written summary
Tomato protoplast electroporation reproduced the satellite accumulation differences.
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- protoplastse0
- Treatment
- CMV-Ix or CMV-1 RNA plus the same satellite variantse0
- Interactor
- CMV-Ixe0pathogen
- Outcome
- differences in accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Electroporation of tomato protoplasts with combinations of CMV-Ix or CMV-1 RNA plus the same satellite variants showed similar differences in accumulation”
Claim · model-written summary
The protoplast result indicated a defect in satellite RNA replication.
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- CMV-Ixe1pathogen
- Outcome
- defect in satellite RNA replicatione0
- Qualifier
- not movement or encapsidatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“indicating a defect in satellite RNA replication and not movement or encapsidation”
e1“Electroporation of tomato protoplasts with combinations of CMV-Ix or CMV-1 RNA plus the same satellite variants”
Claim · model-written summary
Only pseudorecombinants with CMV-Ix RNA 1 failed to replicate satellite RNA.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only combinations with CMV-Ix RNA 1 failed to replicate satellite RNA”
t0“Ixora”
Claim · model-written summary
The CMV-Ix genome was cloned and sequenced.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The complete genome of CMV-Ix was cloned, sequenced and compared with the genomes of other cucumoviruses”
t0“Ixora”
Claim · model-written summary
CMV-Ix was most similar to CMV-Fny and CMV-Y in RNA and protein sequence.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CMV-Ix is most similar in RNA and protein sequence to subgroup 1 CMV-Fny and CMV-Y but slightly less similar than they are to each other”
t0“Ixora”
Claim · model-written summary
Across sequenced cucumoviruses, 39 of 40 bases in a 3-prime untranslated domain were conserved.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CMV-Ix and all cucumovirus strains sequenced thus far share a domain in the 3' untranslated portion of their genomic RNAs in which 39 of 40 bases are completely conserved”
t0“Ixora”
The same questions in other papers
Cucumis sativus · disease · Solanum lycopersicum (tomato)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.