Abstract
The plant hormone abscisic acid (ABA) regulates several physiological and developmental processes in plants, including stress adaptation and seed maturation. ABA-mediated processes appear to be central in plant cold acclimation and expression of cold acclimation-related genes. Ectopic expression of ABI3 encoding a seed-specific transcriptional activator confers on Arabidopsis vegetative tissues the ability to accumulate seed-specific transcripts in response to ABA, and also influences some ABA-mediated vegetative responses. In the present study we characterized the effect of ectopic expression of ABI3 on cold acclimation and development of freezing tolerance in Arabidopsis. We first determined the effect of ABI3 on ABA-induced expression of cold acclimation-related genes. Expression of ABI3 increased the ABA-induced accumulation of transcripts for several ABA/cold/drought-responsive genes such as RAB18 and LTI78. Enhanced expression of these genes was evident even after transient application of ABA, and the enhanced expression was correlated with increased freezing tolerance in ABI3 transgenic plants. Ectopic expression of ABI3 also appeared to modulate low temperature-induced freezing tolerance. The ABI3 transgenic plants acclimated faster than the wild-type plants, and the maximum tolerance obtained was significantly higher. These data showed that lower levels of ABA were needed to trigger the expression of the genes and to maintain the freezing-tolerant state in the ABI3 transgenic plants, and indicate that ectopic expression of ABI3 leads to enhanced responsiveness to ABA. The ectopic expression of ABI3 could provide a new strategy for engineering plant stress tolerance.
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Claims 6
Claim · model-written summary
ABI3 expression increased ABA-induced RAB18 and LTI78 transcript accumulation.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- Expression of ABI3e0
- Gene
- ABI3e0
- Outcome
- ABA-induced accumulation of transcripts for several ABA/cold/drought-responsive genes such as RAB18 and LTI78e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of ABI3 increased the ABA-induced accumulation of transcripts for several ABA/cold/drought-responsive genes such as RAB18 and LTI78”
t0“Arabidopsis thaliana”
Claim · model-written summary
Enhanced cold-response gene expression was evident even after transient ABA application.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- transient application of ABAe0
- Gene
- RAB18 and LTI78e1
- Outcome
- expression of these genese0
- Direction
- Enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Enhanced expression of these genes was evident even after transient application of ABA”
e1“genes such as RAB18 and LTI78”
t0“Arabidopsis thaliana”
Claim · model-written summary
Enhanced gene expression correlated with greater freezing tolerance in ABI3 transgenic plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ABI3e0
- Outcome
- freezing tolerancee0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the enhanced expression was correlated with increased freezing tolerance in ABI3 transgenic plants”
t0“Arabidopsis thaliana”
Claim · model-written summary
ABI3 transgenic plants acclimated faster than wild-type plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- ABI3 transgenic plantse0
- Outcome
- acclimatede0
- Direction
- fastere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ABI3 transgenic plants acclimated faster than the wild-type plants”
t0“Arabidopsis thaliana”
Claim · model-written summary
Maximum freezing tolerance was significantly higher in ABI3 transgenic plants than wild type.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- ABI3 transgenic plantse0
- Outcome
- maximum tolerancee0
- Direction
- highere0
- Comparator
- wild-type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ABI3 transgenic plants acclimated faster than the wild-type plants, and the maximum tolerance obtained was significantly higher”
t0“Arabidopsis thaliana”
Claim · model-written summary
ABI3 transgenic plants needed lower ABA levels to trigger gene expression and maintain freezing tolerance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- ABI3e0
- Outcome
- levels of ABAe0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lower levels of ABA were needed to trigger the expression of the genes and to maintain the freezing-tolerant state in the ABI3 transgenic plants”
t0“Arabidopsis thaliana”
The same questions in other papers
transgenic · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
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- no effect
- no effect
- negative
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.