Abstract
The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro-cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
Trichostatin A increased the fraction of Brassica napus male gametophytes switching to embryogenic growth.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Organ / stage
- male gametophytese0
- Treatment
- trichostatin A (TSA)e0
- Outcome
- proportion of cells that switch from pollen to embryogenic growthe0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth.”
Claim · model-written summary
High-temperature stress enhanced, but was unnecessary for, embryogenic growth.
- Taxon
- B. napusBrassica napuse0
- Treatment
- high-temperature stresse0
- Outcome
- Embryogenic growthe0
- Direction
- enhancede0
- Qualifier
- not dependent one0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus.”
Claim · model-written summary
TSA induced embryogenic cell clusters in Arabidopsis thaliana male gametophytes.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- male gametophytee0
- Treatment
- TSA treatmente0
- Outcome
- embryogenic cell clusterse0
- Direction
- formse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment.”
Claim · model-written summary
Genetic analysis implicated HDA17 in embryogenic switching.
Not reportedTaxon
Verbatim
e0“Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process.”
Claim · model-written summary
TSA treatment was associated with histone H3 and H4 hyperacetylation.
Not reportedTaxon
Verbatim
e0“TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4.”
Claim · model-written summary
The authors propose that an HDAC-dependent mechanism restrains male gametophyte totipotency.
Not reportedTaxon
Verbatim
e0“the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism”
Claim · model-written summary
The authors propose that stress induction acts through the HDAC-dependent pathway.
- Treatment
- stress treatmentse0
- Gene
- HDACe0
- Outcome
- haploid embryo developmente0
- Qualifier
- impinge on this HDAC-dependent pathwaye0
Not reportedTaxon
Verbatim
e0“the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway”
The same questions in other papers
trichostatin · growth · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- positive
trichostatin · molecular: metabolites · Arabidopsis thaliana (thale cress)Results agree
1 paper in the same direction as this paper
- positive
heat · growth · Brassica napus (oilseed rape)Positive and negative results
6 papers in the same direction as this paper · 8 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.