Abstract
Seeds might deteriorate, and lose the ability to germinate when stored under high temperature and high humidity. Brassica napus is one of the most important oil crops in China. However, B. napus seeds were generally stored through summer season with high ambient temperature, which lead to seed viability loss. In order to understand the mechanism of seed response to heat stress and improve seed heat tolerance, B. napus seeds were treated with high temperature (40 °C) and ultra-high temperature (60 °C) for 4 h. The germination of heat-stressed seeds were obviously slower, the germination index and vigor index decreased with temperature increase from 40 to 60 °C, and the 40 °C pretreatment could improve the seed tolerance to 60 °C heat stress. Transcriptomics results showed that 442 differentially expressed genes (DEGs) were identified in seeds after heat stress. Gene ontology and KEGG pathway enrichment analysis revealed that some of the genes were involved in posttranslational modification, protein turnover, chaperones and carbohydrate transport, metabolic pathways and secondary metabolites biosynthesis pathway. Among these DEGs, sHSP and transcription factors genes were involved in heat stress tolerance. Thirty-two overlapping genes under different high temperature stress (40 °C and 60 °C) were enriched in biological processes of response to oxidative stress and abiotic stimulus. The expression trends of 12 genes randomly selected from the RNA-seq data were almost consistent with the results of qRT-PCR. Our results revealed several potential candidate genes and pathways related to heat responsive by high temperature, which is beneficial for further improving the heat tolerance in B. napus seeds.
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Claims 9
Claim · model-written summary
Heat-stressed seeds germinated more slowly.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- heat-stressede0
- Outcome
- germinatione0
- Direction
- slowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“The germination of heat-stressed seeds were obviously slower”
t0“Brassica napus”
Claim · model-written summary
Germination index decreased as temperature rose from 40 to 60 °C.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- temperature increase from 40 to 60 °Ce0
- Outcome
- germination indexe0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the germination index and vigor index decreased with temperature increase from 40 to 60 °C”
t0“Brassica napus”
Claim · model-written summary
Vigor index decreased as temperature rose from 40 to 60 °C.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- temperature increase from 40 to 60 °Ce0
- Outcome
- vigor indexe0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the germination index and vigor index decreased with temperature increase from 40 to 60 °C”
t0“Brassica napus”
Claim · model-written summary
A 40 °C pretreatment improved seed tolerance to 60 °C heat stress.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- 40 °C pretreatmente0
- Outcome
- seed tolerance to 60 °C heat stresse0
- Direction
- improvee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the 40 °C pretreatment could improve the seed tolerance to 60 °C heat stress”
t0“Brassica napus”
Claim · model-written summary
Heat stress yielded 442 differentially expressed seed genes.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- seedse0
- Treatment
- heat stresse0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 442e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“442 differentially expressed genes (DEGs) were identified in seeds after heat stress”
t0“Brassica napus”
Claim · model-written summary
Enrichment implicated posttranslational modification, protein turnover, chaperones, carbohydrate transport, metabolism and secondary metabolite biosynthesis.
- Taxon
- Brassica napusBrassica napust0
- Gene
- genese0
- Outcome
- posttranslational modification, protein turnover, chaperones and carbohydrate transport, metabolic pathways and secondary metabolites biosynthesis pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“some of the genes were involved in posttranslational modification, protein turnover, chaperones and carbohydrate transport, metabolic pathways and secondary metabolites biosynthesis pathway”
t0“Brassica napus”
Claim · model-written summary
sHSP and transcription factor genes were implicated in heat stress tolerance.
- Taxon
- Brassica napusBrassica napust0
- Gene
- sHSP and transcription factors genese0
- Outcome
- heat stress tolerancee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sHSP and transcription factors genes were involved in heat stress tolerance”
t0“Brassica napus”
Claim · model-written summary
Thirty-two genes overlapping the two temperature stresses were enriched in oxidative stress and abiotic stimulus responses.
- Taxon
- Brassica napusBrassica napust0
- Gene
- overlapping genese0
- Outcome
- response to oxidative stress and abiotic stimuluse0
- Value
- Thirty-twoe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thirty-two overlapping genes under different high temperature stress (40 °C and 60 °C) were enriched in biological processes of response to oxidative stress and abiotic stimulus”
t0“Brassica napus”
Claim · model-written summary
RNA-seq and qRT-PCR gave almost consistent expression trends for 12 selected genes.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- expression trendse0
- Value
- 12 genese0
- Comparator
- qRT-PCRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression trends of 12 genes randomly selected from the RNA-seq data were almost consistent with the results of qRT-PCR”
t0“Brassica napus”
The same questions in other papers
heat · growth · Brassica napus (oilseed rape)Positive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.