Abstract
The root stem cell niche, which in the Arabidopsis thaliana root meristem is an area of four mitotically inactive quiescent cells (QCs) and the surrounding mitotically active stem cells, is critical for root development and growth. We report here that during jasmonate-induced inhibition of primary root growth, jasmonate reduces root meristem activity and leads to irregular QC division and columella stem cell differentiation. Consistently, jasmonate reduces the expression levels of the AP2-domain transcription factors PLETHORA1 (PLT1) and PLT2, which form a developmentally instructive protein gradient and mediate auxin-induced regulation of stem cell niche maintenance. Not surprisingly, the effects of jasmonate on root stem cell niche maintenance and PLT expression require the functioning of MYC2/JASMONATE INSENSITIVE1, a basic helix-loop-helix transcription factor that involves versatile aspects of jasmonate-regulated gene expression. Gel shift and chromatin immunoprecipitation experiments reveal that MYC2 directly binds the promoters of PLT1 and PLT2 and represses their expression. We propose that MYC2-mediated repression of PLT expression integrates jasmonate action into the auxin pathway in regulating root meristem activity and stem cell niche maintenance. This study illustrates a molecular framework for jasmonate-induced inhibition of root growth through interaction with the growth regulator auxin.
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Claims 6
Claim · model-written summary
Jasmonate reduced root meristem activity.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae1 - Treatment
- jasmonatee0
- Outcome
- root meristem activitye0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonate reduces root meristem activity and leads to irregular QC division and columella stem cell differentiation”
e1“Arabidopsis thaliana root meristem”
Claim · model-written summary
Jasmonate led to irregular QC division and columella stem cell differentiation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- jasmonatee0
- Outcome
- irregular QC division and columella stem cell differentiatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonate reduces root meristem activity and leads to irregular QC division and columella stem cell differentiation”
e1“Arabidopsis thaliana root meristem”
Claim · model-written summary
Jasmonate reduced PLT1 and PLT2 expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- jasmonatee0
- Gene
- PLETHORA1 (PLT1) and PLT2e0
- Outcome
- expression levelse0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonate reduces the expression levels of the AP2-domain transcription factors PLETHORA1 (PLT1) and PLT2”
e1“Arabidopsis thaliana root meristem”
Claim · model-written summary
MYC2 was required for jasmonate effects on stem cell niche maintenance and PLT expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Treatment
- jasmonatee0
- Gene
- MYC2/JASMONATE INSENSITIVE1e0
- Outcome
- root stem cell niche maintenance and PLT expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the effects of jasmonate on root stem cell niche maintenance and PLT expression require the functioning of MYC2/JASMONATE INSENSITIVE1”
e1“Arabidopsis thaliana root meristem”
Claim · model-written summary
MYC2 bound PLT1 and PLT2 promoters and repressed their expression.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- MYC2e0
- Outcome
- their expressione0
- Direction
- repressese0
- Qualifier
- binds the promoters of PLT1 and PLT2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYC2 directly binds the promoters of PLT1 and PLT2 and represses their expression”
e1“Arabidopsis thaliana root meristem”
Claim · model-written summary
The authors proposed that MYC2-mediated PLT repression integrates jasmonate and auxin regulation of meristem and stem cell niche maintenance.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae1
- Gene
- MYC2e0
- Outcome
- root meristem activity and stem cell niche maintenancee0
- Qualifier
- integrates jasmonate action into the auxin pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We propose that MYC2-mediated repression of PLT expression integrates jasmonate action into the auxin pathway in regulating root meristem activity and stem cell niche maintenance”
e1“Arabidopsis thaliana root meristem”
The same questions in other papers
jasmonic acid · molecular: expression · Arabidopsis thaliana (thale cress)Positive and negative results
3 papers in the same direction as this paper · 10 in the opposite direction
- no effect
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.