Abstract
Sarawak Biris rice is a strong aromatic rice variety which can thrive well in rice fields prone to flood, drought and other soil constraints. Tissue culture of Biris rice is important in producing planting materials and conducting genetic improvement work. The present study was conducted to induce callus from Biris rice seed and assess its regeneration ability. Dehusked mature seeds were used as starting materials for callus induction. Sterilized seeds were cultured onto Murashige and Skoog medium containing various concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D). The optimum 2, 4-D concentration for callus induction was 2.0 mg/L with a frequency as high as 97%. The calli produced were of desired features (creamy in colour, globular) and relatively bigger in size as compared to other treatments. The calli produced were further tested with plant growth regulator, naphthaleneacetic acid (NAA) in combination with kinetin (Kn) for plant regeneration ability. All uncontaminated calli were found to regenerate. Up to nine shoots were induced by a callus treated with 0.5 mg/L NAA in combination with 1.0 mg/L Kn. The present study should be noted as the first attempt to induce callus and regenerate plants from seeds of Biris rice. Key words: Biris rice, Sarawak, callus induction, plant regeneration.
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Claims 4
Claim · model-written summary
Callus induction from Biris rice seeds was highest at the optimum 2,4-D concentration of 2.0 mg/L, with a frequency of 97%.
- Taxon
- Oryza sativa L.
Oryza sativat0 - Outcome
- callus inductione1
- Direction
- higherinferrede1
- Value
- 97%e1
- Comparator
- various concentrationse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus induction and plant regeneration of Sarawak rice (Oryza sativa L.) variety Biris”
e1“The optimum 2, 4-D concentration for callus induction was 2.0 mg/L with a frequency as high as 97%”
e2“Sterilized seeds were cultured onto Murashige and Skoog medium containing various concentrations of 2, 4-dichlorophenoxyacetic acid (2, 4-D)”
Claim · model-written summary
Calli produced on 2,4-D had desired features and were relatively bigger in size than calli from other treatments.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- callie3
- Outcome
- relatively bigger in sizee3
- Qualifier
- creamy in colour, globulare3
- Comparator
- other treatmentse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus induction and plant regeneration of Sarawak rice (Oryza sativa L.) variety Biris”
e3“The calli produced were of desired features (creamy in colour, globular) and relatively bigger in size as compared to other treatments”
Claim · model-written summary
All uncontaminated calli of Biris rice regenerated.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- callie4
- Outcome
- regeneratee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus induction and plant regeneration of Sarawak rice (Oryza sativa L.) variety Biris”
e4“All uncontaminated calli were found to regenerate”
Claim · model-written summary
Up to nine shoots were induced from a callus treated with 0.5 mg/L NAA and 1.0 mg/L kinetin.
- Taxon
- Oryza sativa L.Oryza sativat0
- Organ / stage
- calluse5
- Treatment
- 0.5 mg/L NAA in combination with 1.0 mg/L Kne5
- Outcome
- shootse5
- Value
- Up to ninee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Callus induction and plant regeneration of Sarawak rice (Oryza sativa L.) variety Biris”
e5“Up to nine shoots were induced by a callus treated with 0.5 mg/L NAA in combination with 1.0 mg/L Kn”
The same questions in other papers
2,4-D · growth · Oryza sativa (rice)Positive and negative results
101 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
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See all 107 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.