Abstract
Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium. Callus induction frequency was different between the cultivars, as well as among the 2,4-dichlorophenoxyacetic acid (2,4-D) levels tested. Both tested cultivars exhibited highest callus frequency at 2 mgl-1 2,4-D. The incorporation of benzylaminopurine (BAP) and kinetin (KIN) in the callus induction medium supplemented with 2 mgl-1 2,4-D did not significantly improve the callus induction frequency but required days of callus initiation were decreased compared to single use of 2,4-D. After two subcultures, at 21 days interval, embryogenic callus was placed on medium containing different concentration and combination of auxin and cytokinin. Treatment T4 (0.5 mg l-1 BAP and 0.1 mg l-1 IBA) showed the highest shoot induction: 91.67% in Kalijira and 83.33% in Chinigura. Similarly, the highest range of shoot number was also observed in both genotypes when treated with 0.5 mgl-1 BAP and 0.1 mgl-1 IBA. Plant regeneration efficiency was further observed best when treated with 1 mgl-1 2,4-D along with 1 mgl-1 2,4-D along with 1 mgl-1 BAP and 1 mgl-1 IBA. Furthermore, the highest number of callus derived shoot per culture was achieved in 2 mgl-1 2,4-D along with 1 mgl-1 BAP and 1 mgl-1 IBA. Both rice genotypes are promising in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus.
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Claims 8
Claim · model-written summary
Callus frequency was highest at 2 mgl-1 2,4-D in both rice cultivars.
- Taxon
- rice
Oryza sativae0 - Material
- Kalijira and Chinigurae0
- Treatment
- 2 mgl-1 2,4-De2
- Outcome
- callus frequencye2
- Direction
- higheste2
- Comparator
- (2,4-D) levels testede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium”
e1“Callus induction frequency was different between the cultivars, as well as among the 2,4-dichlorophenoxyacetic acid (2,4-D) levels tested”
e2“Both tested cultivars exhibited highest callus frequency at 2 mgl-1 2,4-D”
Claim · model-written summary
Callus induction frequency differed between the two rice cultivars.
- Taxon
- riceOryza sativae0
- Outcome
- Callus induction frequencye1
- Qualifier
- different between the cultivarse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium”
e1“Callus induction frequency was different between the cultivars, as well as among the 2,4-dichlorophenoxyacetic acid (2,4-D) levels tested”
Claim · model-written summary
Adding BAP and KIN to 2 mgl-1 2,4-D medium did not significantly improve callus induction frequency.
- Taxon
- riceOryza sativae0
- Treatment
- incorporation of benzylaminopurine (BAP) and kinetin (KIN)e3
- Outcome
- callus induction frequencye3
- Direction
- did not significantly improvee3
- Comparator
- 2 mgl-1 2,4-De3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium”
e3“The incorporation of benzylaminopurine (BAP) and kinetin (KIN) in the callus induction medium supplemented with 2 mgl-1 2,4-D did not significantly improve the callus induction frequency but required days of callus initiation were decreased compared to single use of 2,4-D”
Claim · model-written summary
Adding BAP and KIN to 2 mgl-1 2,4-D medium decreased the days required for callus initiation compared with single 2,4-D.
- Taxon
- riceOryza sativae0
- Treatment
- incorporation of benzylaminopurine (BAP) and kinetin (KIN)e3
- Outcome
- required days of callus initiatione3
- Direction
- decreasede3
- Comparator
- single use of 2,4-De3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium”
e3“The incorporation of benzylaminopurine (BAP) and kinetin (KIN) in the callus induction medium supplemented with 2 mgl-1 2,4-D did not significantly improve the callus induction frequency but required days of callus initiation were decreased compared to single use of 2,4-D”
Claim · model-written summary
Treatment T4 gave the highest shoot induction in Kalijira (91.67%).
- Taxon
- riceOryza sativae0
- Material
- Kalijirae4
- Treatment
- Treatment T4 (0.5 mg l-1 BAP and 0.1 mg l-1 IBA)e4
- Outcome
- shoot inductione4
- Direction
- higheste4
- Value
- 91.67%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium”
e4“Treatment T4 (0.5 mg l-1 BAP and 0.1 mg l-1 IBA) showed the highest shoot induction: 91.67% in Kalijira and 83.33% in Chinigura”
Claim · model-written summary
Treatment T4 gave the highest shoot induction in Chinigura (83.33%).
- Taxon
- riceOryza sativae0
- Material
- Chinigurae4
- Treatment
- Treatment T4 (0.5 mg l-1 BAP and 0.1 mg l-1 IBA)e4
- Outcome
- shoot inductione4
- Direction
- higheste4
- Value
- 83.33%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium”
e4“Treatment T4 (0.5 mg l-1 BAP and 0.1 mg l-1 IBA) showed the highest shoot induction: 91.67% in Kalijira and 83.33% in Chinigura”
Claim · model-written summary
0.5 mgl-1 BAP and 0.1 mgl-1 IBA gave the highest range of shoot number in both rice genotypes.
- Taxon
- riceOryza sativae0
- Treatment
- 0.5 mgl-1 BAP and 0.1 mgl-1 IBAe5
- Outcome
- shoot numbere5
- Direction
- highest rangee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mature seeds of two traditional rice genotypes (Kalijira and Chinigura) were used for callus induction and plant regeneration on different concentrations and combinations of plant growth regulators cultured on MS (Murashige and Skoog) basal medium”
e5“Similarly, the highest range of shoot number was also observed in both genotypes when treated with 0.5 mgl-1 BAP and 0.1 mgl-1 IBA”
Claim · model-written summary
Both rice genotypes are promising for callus induction frequency, morphology and regeneration ability.
- Taxon
- rice genotypesOryza sativae7
- Outcome
- promising in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic calluse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“Both rice genotypes are promising in terms of callus induction frequency and morphology, and regeneration ability of the embryogenic callus”
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