Abstract
Agrobacterium -mediated transformation of indica rice has been established in only a limited number of cultivars because the regeneration of plants from transformed embryogenic calli is highly cultivar-specific. Establishment of a highly efficient plant regeneration system from shoot apex explants applicable to many cultivars of indica rice will accelerate the application of transformation technology in breeding programs and functional genomics study. We established an efficient shoot multiplication and plant regeneration system from shoot apices of indica rice using thidiazuron (TDZ) as a plant growth regulator. Shoot apices cultured on MS basal medium devoid of plant growth regulators formed solitary shoots in 90% of cultures. Addition of TDZ or benzylaminopurine to regeneration medium significantly influenced formation of multiple shoots directly from shoot apex explants without an intervening callus stage. Best shoot proliferation response (10.3 shoots per explant) was recorded when shoot apices were cultured on media supplemented with 4 mg/l TDZ. No synergistic effect on shoot proliferation was observed when indole-3-acetic acid and indole-3-butyric acid were supplemented to media containing 4 mg/l TDZ. The regeneration system was efficient in evoking multiple shoot proliferation in eight different cultivars of indica rice. Shoots were rooted in MS basal medium and plantlets were acclimatized with 100% survival rate. The shoot apex explants of all the eight cultivars of indica rice were found competent to Agrobacterium -mediated transformation while explants from IR-64 showed highest transient GUS expression. This variety-independent transformation amenable regeneration system from shoot apices may widely be applicable for genetic transformation of indica varieties.
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Claims 9
Claim · model-written summary
Shoot apices cultured on MS basal medium devoid of plant growth regulators formed solitary shoots in 90% of cultures
- Taxon
- rice
Oryza sativat0 - Organ / stage
- Shoot apicese0
- Treatment
- MS basal medium devoid of plant growth regulatorse0
- Outcome
- solitary shootse0
- Value
- 90%e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot apices cultured on MS basal medium devoid of plant growth regulators formed solitary shoots in 90% of cultures”
t0“rice”
Claim · model-written summary
Addition of TDZ or benzylaminopurine to regeneration medium significantly influenced formation of multiple shoots directly from shoot apex explants without an intervening callus stage
- Taxon
- riceOryza sativat0
- Treatment
- TDZe0
- Outcome
- formation of multiple shootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of TDZ or benzylaminopurine to regeneration medium significantly influenced formation of multiple shoots directly from shoot apex explants without an intervening callus stage”
t0“rice”
Claim · model-written summary
Addition of TDZ or benzylaminopurine to regeneration medium significantly influenced formation of multiple shoots directly from shoot apex explants without an intervening callus stage
- Taxon
- riceOryza sativat0
- Treatment
- benzylaminopurinee0
- Outcome
- formation of multiple shootse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Addition of TDZ or benzylaminopurine to regeneration medium significantly influenced formation of multiple shoots directly from shoot apex explants without an intervening callus stage”
t0“rice”
Claim · model-written summary
Best shoot proliferation response (10.3 shoots per explant) was recorded when shoot apices were cultured on media supplemented with 4 mg/l TDZ
- Taxon
- riceOryza sativat0
- Treatment
- 4 mg/l TDZe0
- Outcome
- shoot proliferation responsee0
- Direction
- Beste0
- Value
- 10.3 shoots per explante0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Best shoot proliferation response (10.3 shoots per explant) was recorded when shoot apices were cultured on media supplemented with 4 mg/l TDZ”
t0“rice”
Claim · model-written summary
No synergistic effect on shoot proliferation was observed when indole-3-acetic acid and indole-3-butyric acid were supplemented to media containing 4 mg/l TDZ
- Taxon
- riceOryza sativat0
- Treatment
- indole-3-acetic acid and indole-3-butyric acide0
- Outcome
- shoot proliferatione0
- Direction
- No synergistic effecte0
- Comparator
- 4 mg/l TDZe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No synergistic effect on shoot proliferation was observed when indole-3-acetic acid and indole-3-butyric acid were supplemented to media containing 4 mg/l TDZ”
t0“rice”
Claim · model-written summary
The regeneration system was efficient in evoking multiple shoot proliferation in eight different cultivars of indica rice
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Outcome
- multiple shoot proliferatione0
- Value
- eight different cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regeneration system was efficient in evoking multiple shoot proliferation in eight different cultivars of indica rice”
Claim · model-written summary
plantlets were acclimatized with 100% survival rate
- Taxon
- riceOryza sativat0
- Outcome
- survival ratee0
- Value
- 100%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plantlets were acclimatized with 100% survival rate”
t0“rice”
Claim · model-written summary
shoot apex explants of all the eight cultivars of indica rice were found competent to Agrobacterium -mediated transformation
- Taxon
- indica riceOryza sativa Indica Groupe0
- Interactor
- Agrobacterium
.jpg?width=60)
Agrobacteriume0other - Outcome
- competent to Agrobacterium -mediated transformatione0
- Value
- eight cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“shoot apex explants of all the eight cultivars of indica rice were found competent to Agrobacterium -mediated transformation”
Claim · model-written summary
explants from IR-64 showed highest transient GUS expression
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“explants from IR-64 showed highest transient GUS expression”
t0“rice”
The same questions in other papers
indole-3-butyric acid · growth · Oryza sativa (rice)Positive and negative results
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See all 11 papers →indole-3-acetic acid · growth · Oryza sativa (rice)Positive and negative results
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See all 47 papers →Thidiazuron · growth · Oryza sativa (rice)Results agree
2 papers in the same direction as this paper
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.