Abstract
Endophytic fungi are known to produce indole-3-acetic acid (IAA), which can stimulate plant growth. Twenty-seven isolates of endophytic fungi were isolated from Coffea arabica in northern Thailand. Only one isolate (CMU-A109) produced IAA in vitro. This isolate was identified as Colletotrichum fructicola based on morphological characteristics and molecular phylogenetic analysis of a combined five loci (internal transcribed spacer of ribosomal DNA, actin, β-tubulin 2, chitin synthase and glyceraldehyde-3-phosphate dehydrogenase genes). Identification of a fungal IAA production obtained from indole 3-acetamide (IAM) and tryptophan 2-monooxygenase activity is suggestive of IAM routed IAA biosynthesis. The highest IAA yield (1205.58±151.89 μg/mL) was obtained after 26 days of cultivation in liquid medium supplemented with 8 mg/mL L-tryptophan at 30°C. Moreover, the crude fungal IAA could stimulate coleoptile elongation of maize, rice and rye. This is the first report of IAA production by C. fructicola and its ability to produce IAA was highest when compared with previous reports on IAA produced by fungi.
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Claims 7
Claim · model-written summary
One coffee derived fungal isolate produced IAA in vitro.
- Taxon
- Coffea arabica
Coffea arabicae0 - Material
- CMU-A109e0
- Interactor
- endophytic fungie0symbiont
- Outcome
- produced IAA in vitroe0
- Value
- one isolatee0
- Setting
- northern Thailande0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Twenty-seven isolates of endophytic fungi were isolated from Coffea arabica in northern Thailand. Only one isolate (CMU-A109) produced IAA in vitro.”
Claim · model-written summary
The IAA producing isolate was identified as Colletotrichum fructicola.
- Taxon
- Coffea arabicaCoffea arabicae0
- Interactor
- Colletotrichum fructicola
_Figure_8.jpg?width=60)
Colletotrichum fructicolae1symbiont - Outcome
- identified as Colletotrichum fructicolae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Figure_8.jpg?width=500)
Verbatim
e0“isolates of endophytic fungi were isolated from Coffea arabica”
e1“This isolate was identified as Colletotrichum fructicola based on morphological characteristics and molecular phylogenetic analysis”
Claim · model-written summary
The authors infer an IAM route for fungal IAA biosynthesis.
- Taxon
- Coffea arabicaCoffea arabicae0
- Gene
- tryptophan 2-monooxygenasee1
- Compound
- indole 3-acetamidee1
- Outcome
- IAA biosynthesise1
- Qualifier
- IAM routede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from Coffea arabica”
e1“Identification of a fungal IAA production obtained from indole 3-acetamide (IAM) and tryptophan 2-monooxygenase activity is suggestive of IAM routed IAA biosynthesis.”
Claim · model-written summary
The highest fungal IAA yield was 1205.58±151.89 μg/mL after 26 days with L-tryptophan at 30°C.
- Taxon
- Coffea arabicaCoffea arabicae0
- Treatment
- 8 mg/mL L-tryptophane1
- Outcome
- IAA yielde1
- Direction
- higheste1
- Value
- 1205.58±151.89 μg/mLe1
- Qualifier
- after 26 days of cultivation in liquid mediume1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“isolated from Coffea arabica”
e1“The highest IAA yield (1205.58±151.89 μg/mL) was obtained after 26 days of cultivation in liquid medium supplemented with 8 mg/mL L-tryptophan at 30°C.”
Claim · model-written summary
Crude fungal IAA stimulated maize coleoptile elongation.
Zea maysNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the crude fungal IAA could stimulate coleoptile elongation of maize, rice and rye.”
Claim · model-written summary
Crude fungal IAA stimulated rice coleoptile elongation.
- Taxon
- rice
Oryza sativae0 - Treatment
- crude fungal IAAe0
- Outcome
- coleoptile elongatione0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the crude fungal IAA could stimulate coleoptile elongation of maize, rice and rye.”
Claim · model-written summary
Crude fungal IAA stimulated rye coleoptile elongation.
- Taxon
- rye
Secale cerealee0 - Treatment
- crude fungal IAAe0
- Outcome
- coleoptile elongatione0
- Direction
- stimulatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the crude fungal IAA could stimulate coleoptile elongation of maize, rice and rye.”
The same questions in other papers
indole-3-acetic acid · growth · Zea mays (maize)Results differ
34 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- no effect
37 papers more in this group
See all 41 papers →indole-3-acetic acid · growth · Oryza sativa (rice)Positive and negative results
39 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- negative
- no effect
- no effect
43 papers more in this group
See all 47 papers →indole-3-acetic acid · growth · Secale cereale (rye)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.