Abstract
The role of sucrose synthase (SuSy) in tomato fruit was studied in transgenic tomato (Lycopersicon esculentum) plants expressing an antisense fragment of fruit-specific SuSy RNA (TOMSSF) under the control of the cauliflower mosaic virus 35S promoter. Constitutive expression of the antisense RNA markedly inhibited SuSy activity in flowers and fruit pericarp tissues. However, inhibition was only slight in the endosperm and was undetectable in the embryo, shoot, petiole, and leaf tissues. The activity of sucrose phosphate synthase decreased in parallel with that of SuSy, but acid invertase activity did not increase in response to the reduced SuSy activity. The only effect on the carbohydrate content of young fruit was a slight reduction in starch accumulation. The in vitro sucrose import capacity of fruits was not reduced by SuSy inhibition at 23 days after anthesis, and the rate of starch synthesized from the imported sucrose was not lessened even when SuSy activity was decreased by 98%. However, the sucrose unloading capacity of 7-day-old fruit was substantially decreased in lines with low SuSy activity. In addition, the SuSy antisense fruit from the first week of flowering had a slower growth rate. A reduced fruit set, leading to markedly less fruit per plant at maturity, was observed for the plants with the least SuSy activity. These results suggest that SuSy participates in the control of sucrose import capacity of young tomato fruit, which is a determinant for fruit set and development.
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Claims 12
Claim · model-written summary
Antisense expression markedly inhibited SuSy activity in flowers and pericarp.
- Taxon
- tomato
Solanum lycopersicumt0 - Organ / stage
- flowers and fruit pericarp tissuese0
- Treatment
- Constitutive expression of the antisense RNAe0
- Gene
- SuSye0
- Outcome
- SuSy activitye0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive expression of the antisense RNA markedly inhibited SuSy activity in flowers and fruit pericarp tissues”
t0“Tomato”
Claim · model-written summary
SuSy inhibition was slight in endosperm.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- endosperme0
- Treatment
- inhibitione0
- Outcome
- inhibitione0
- Direction
- slighte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, inhibition was only slight in the endosperm”
t0“Tomato”
Claim · model-written summary
SuSy inhibition was undetectable in embryo, shoot, petiole and leaf tissues.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- undetectable in the embryo, shoot, petiole, and leaf tissuese0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was undetectable in the embryo, shoot, petiole, and leaf tissues”
t0“Tomato”
Claim · model-written summary
Sucrose phosphate synthase activity decreased with SuSy activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- sucrose phosphate synthasee0
- Outcome
- activity of sucrose phosphate synthasee0
- Direction
- decreasede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activity of sucrose phosphate synthase decreased in parallel with that of SuSy”
t0“Tomato”
Claim · model-written summary
Acid invertase activity did not increase when SuSy activity was reduced.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- reduced SuSy activitye0
- Gene
- acid invertasee0
- Outcome
- acid invertase activitye0
- Direction
- did not increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“acid invertase activity did not increase in response to the reduced SuSy activity”
t0“Tomato”
Claim · model-written summary
Young fruit starch accumulation was slightly reduced.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- young fruite0
- Outcome
- starch accumulatione0
- Direction
- reductione0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The only effect on the carbohydrate content of young fruit was a slight reduction in starch accumulation”
t0“Tomato”
Claim · model-written summary
SuSy inhibition did not reduce in vitro sucrose import capacity at 23 days after anthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- 23 days after anthesise0
- Treatment
- SuSy inhibitione0
- Outcome
- in vitro sucrose import capacity of fruitse0
- Direction
- not reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The in vitro sucrose import capacity of fruits was not reduced by SuSy inhibition at 23 days after anthesis”
t0“Tomato”
Claim · model-written summary
Starch synthesis from imported sucrose was not lessened even at 98% lower SuSy activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- SuSy activity was decreased by 98%e0
- Outcome
- rate of starch synthesized from the imported sucrosee0
- Direction
- not lessenede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rate of starch synthesized from the imported sucrose was not lessened even when SuSy activity was decreased by 98%”
t0“Tomato”
Claim · model-written summary
Sucrose unloading capacity of 7-day-old fruit decreased in low SuSy lines.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- 7-day-old fruite0
- Treatment
- low SuSy activitye0
- Outcome
- sucrose unloading capacitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the sucrose unloading capacity of 7-day-old fruit was substantially decreased in lines with low SuSy activity”
t0“Tomato”
Claim · model-written summary
SuSy antisense fruit grew more slowly during the first flowering week.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- first week of floweringe0
- Treatment
- SuSy antisensee0
- Outcome
- growth ratee0
- Direction
- slowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the SuSy antisense fruit from the first week of flowering had a slower growth rate”
t0“Tomato”
Claim · model-written summary
Plants with the least SuSy activity had reduced fruit set.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- least SuSy activitye0
- Outcome
- fruit sete0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reduced fruit set, leading to markedly less fruit per plant at maturity, was observed for the plants with the least SuSy activity”
t0“Tomato”
Claim · model-written summary
Plants with the least SuSy activity had fewer mature fruits per plant.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- least SuSy activitye0
- Outcome
- fruit per plant at maturitye0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reduced fruit set, leading to markedly less fruit per plant at maturity, was observed for the plants with the least SuSy activity”
t0“Tomato”
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