Abstract
Plant phosphorus (P) diagnosis is widely used for monitoring P status and guiding P fertilizer application in field conditions. The common methods for predicting plant response to P are time- and labour-consuming chemical measurements of the extractable soil P and plant P concentrations. In this study, we successfully generated a visual reporter system in tobacco (Nicotiana tabacum L.) to monitor plant P status by expressing of a Purple gene (Pr) isolated from cauliflower (Brassica oleracea var botrytis) driven by the promoter (Pro) of OsPT6, a P-starvation-induced rice gene. The leaves of OsPT6pro::Pr (PT6pro::Pr) transgenic tobacco continuously turned into dark purple with the increase of duration and severity of P deficiency, and recovered rapidly to basal green colour upon resupply of P. The expression of several anthocyanin biosynthesis involving genes was strongly activated in the transgenic tobacco in comparison to wild type under P-deficient condition. Such additive purple colour was not detected by deficiencies of other major- and micronutrients or stresses of salt, drought and cold. There was an extremely high correlation between P concentration and anthocyanin accumulation in the transgenic tobacco leaves. Using a hyperspectral sensing technology, P concentration in the leaves of transgenic plants could be predicted by the reflectance spectra at 554 nm wavelength with approximately 0.16 as the threshold value of the P deficiency. Taken together, the colour-based visual reporter system could be specifically and readily used for monitoring the plant P status by naked eyes and accurately assessed by spectral reflectance.
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Claims 6
Claim · model-written summary
Under P deficiency, OsPT6pro::Pr transgenic tobacco leaves turned dark purple, increasingly with duration and severity of deficiency.
- Taxon
- Nicotiana tabacum
Nicotiana tabacume0 - Material
- OsPT6pro::Pre1
- Organ / stage
- leavese1
- Treatment
- P deficiencye1
- Outcome
- turned into dark purplee1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e1“The leaves of OsPT6pro::Pr (PT6pro::Pr) transgenic tobacco continuously turned into dark purple with the increase of duration and severity of P deficiency”
Claim · model-written summary
Several anthocyanin biosynthesis genes were strongly activated in transgenic tobacco compared with wild type under P deficiency.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- P-deficient conditione2
- Gene
- anthocyanin biosynthesis involving genese2
- Outcome
- expression of several anthocyanin biosynthesis involving genese2
- Direction
- strongly activatede2
- Comparator
- wild typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e2“The expression of several anthocyanin biosynthesis involving genes was strongly activated in the transgenic tobacco in comparison to wild type under P-deficient condition”
Claim · model-written summary
Leaf P concentration and anthocyanin accumulation were extremely highly correlated in transgenic tobacco leaves.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Organ / stage
- leavese3
- Outcome
- anthocyanin accumulatione3
- Qualifier
- extremely high correlatione3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e3“There was an extremely high correlation between P concentration and anthocyanin accumulation in the transgenic tobacco leaves”
Claim · model-written summary
Leaf P concentration of transgenic tobacco could be predicted from reflectance at 554 nm, with about 0.16 as the P-deficiency threshold.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Outcome
- P concentratione4
- Value
- approximately 0.16e4
- Qualifier
- reflectance spectra at 554 nm wavelengthe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e4“P concentration in the leaves of transgenic plants could be predicted by the reflectance spectra at 554 nm wavelength with approximately 0.16 as the threshold value of the P deficiency”
Claim · model-written summary
The purple colour was not produced by deficiencies of other major or micronutrients, or by salt, drought or cold stress.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- deficiencies of other major- and micronutrients or stresses of salt, drought and colde5
- Outcome
- additive purple coloure5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e5“Such additive purple colour was not detected by deficiencies of other major- and micronutrients or stresses of salt, drought and cold”
Claim · model-written summary
Leaves recovered rapidly to basal green colour when P was resupplied.
- Taxon
- Nicotiana tabacumNicotiana tabacume0
- Treatment
- resupply of Pe6
- Outcome
- basal green coloure6
- Direction
- recovered rapidlye6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tobacco (Nicotiana tabacum L.)”
e6“recovered rapidly to basal green colour upon resupply of P”
The same questions in other papers
drought · quality: postharvest · Nicotiana tabacum (tobacco)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.