Abstract
Although flowering regulatory mechanisms have been extensively studied in Arabidopsis (Arabidopsis thaliana), those in other species have not been well elucidated. Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa). In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering. Transcript levels of three flowering regulators-Ehd1, OsMADS14, and Hd3a-were decreased in these mutants, whereas those of OsGI and Hd1 were unchanged. Ectopic expression of OsMADS51 caused flowering to occur about 7 d earlier only in SDs. In ectopic expression lines, transcript levels of Ehd1, OsMADS14, and Hd3a were increased, but those of OsGI and Hd1 remained the same. These results indicate that OsMADS51 is a flowering promoter, particularly in SDs, and that this gene functions upstream of Ehd1, OsMADS14, and Hd3a. To further investigate the relationship with other flowering promoters, we generated transgenic plants in which expression of Ehd1 or OsGI was suppressed. In Ehd1 RNA interference plants, OsMADS51 expression was not affected, supporting our conclusion that the MADS-box gene functions upstream of Ehd1. However, in OsGI antisense plants, the OsMADS51 transcript level was reduced. In addition, the circadian expression pattern for this MADS-box gene was similar to that for OsGI. These results demonstrate that OsMADS51 functions downstream of OsGI. In summary, OsMADS51 is a novel flowering promoter that transmits a SD promotion signal from OsGI to Ehd1.
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Claims 12
Claim · model-written summary
OsMADS51 null mutants flowered 2 weeks later than normal in short days in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- OsMADS51 null mutantse1
- Outcome
- flowerede1
- Direction
- latere1
- Value
- 2 weekse1
- Qualifier
- In SDse1
- Comparator
- normale1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e1“In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering”
Claim · model-written summary
Loss of OsMADS51 had little effect on flowering in long days in rice.
- Taxon
- riceOryza sativae0
- Treatment
- loss of OsMADS51e1
- Outcome
- floweringe1
- Direction
- no_changeinferrede1
- Qualifier
- in long dayse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e1“In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering”
Claim · model-written summary
Transcript levels of Ehd1, OsMADS14 and Hd3a decreased in OsMADS51 null mutants of rice.
- Taxon
- riceOryza sativae0
- Treatment
- OsMADS51 null mutantse1
- Gene
- Ehd1, OsMADS14, and Hd3ae2
- Outcome
- Transcript levelse2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e1“In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering”
e2“Transcript levels of three flowering regulators-Ehd1, OsMADS14, and Hd3a-were decreased in these mutants, whereas those of OsGI and Hd1 were unchanged”
Claim · model-written summary
Transcript levels of OsGI and Hd1 were unchanged in OsMADS51 null mutants of rice.
- Taxon
- riceOryza sativae0
- Treatment
- OsMADS51 null mutantse1
- Gene
- OsGI and Hd1e2
- Outcome
- Transcript levelse2
- Direction
- unchangede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e1“In SDs OsMADS51 null mutants flowered 2 weeks later than normal, whereas in long days loss of OsMADS51 had little effect on flowering”
e2“Transcript levels of three flowering regulators-Ehd1, OsMADS14, and Hd3a-were decreased in these mutants, whereas those of OsGI and Hd1 were unchanged”
Claim · model-written summary
Ectopic expression of OsMADS51 advanced flowering by about 7 d in short days in rice.
- Taxon
- riceOryza sativae0
- Treatment
- Ectopic expression of OsMADS51e3
- Outcome
- floweringe3
- Direction
- earliere3
- Value
- about 7 de3
- Qualifier
- only in SDse3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e3“Ectopic expression of OsMADS51 caused flowering to occur about 7 d earlier only in SDs”
Claim · model-written summary
Transcript levels of Ehd1, OsMADS14 and Hd3a increased in ectopic expression lines of rice.
- Taxon
- riceOryza sativae0
- Treatment
- ectopic expression linese4
- Gene
- Ehd1, OsMADS14, and Hd3ae4
- Outcome
- transcript levelse4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e4“In ectopic expression lines, transcript levels of Ehd1, OsMADS14, and Hd3a were increased, but those of OsGI and Hd1 remained the same”
Claim · model-written summary
Transcript levels of OsGI and Hd1 remained the same in ectopic expression lines of rice.
- Taxon
- riceOryza sativae0
- Treatment
- ectopic expression linese4
- Gene
- OsGI and Hd1e4
- Outcome
- transcript levelse4
- Direction
- remained the samee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e4“In ectopic expression lines, transcript levels of Ehd1, OsMADS14, and Hd3a were increased, but those of OsGI and Hd1 remained the same”
Claim · model-written summary
OsMADS51 expression was not affected in Ehd1 RNA interference plants of rice.
- Taxon
- riceOryza sativae0
- Treatment
- Ehd1 RNA interferencee5
- Gene
- OsMADS51e5
- Outcome
- OsMADS51 expressione5
- Direction
- not affectede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e5“In Ehd1 RNA interference plants, OsMADS51 expression was not affected, supporting our conclusion that the MADS-box gene functions upstream of Ehd1”
Claim · model-written summary
OsMADS51 functions upstream of Ehd1 in the rice flowering pathway.
- Taxon
- riceOryza sativae0
- Gene
- OsMADS51e5
- Outcome
- functions upstream of Ehd1e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e5“In Ehd1 RNA interference plants, OsMADS51 expression was not affected, supporting our conclusion that the MADS-box gene functions upstream of Ehd1”
Claim · model-written summary
OsMADS51 transcript level was reduced in OsGI antisense plants of rice.
- Taxon
- riceOryza sativae0
- Treatment
- OsGI antisense plantse6
- Gene
- OsMADS51e6
- Outcome
- OsMADS51 transcript levele6
- Direction
- reducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e6“in OsGI antisense plants, the OsMADS51 transcript level was reduced”
Claim · model-written summary
The circadian expression pattern of the MADS-box gene was similar to that of OsGI in rice.
- Taxon
- riceOryza sativae0
- Outcome
- circadian expression patterne7
- Direction
- similare7
- Comparator
- OsGIe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e7“the circadian expression pattern for this MADS-box gene was similar to that for OsGI”
Claim · model-written summary
OsMADS51 functions downstream of OsGI in the rice flowering pathway.
- Taxon
- riceOryza sativae0
- Gene
- OsMADS51e7
- Outcome
- functions downstream of OsGIe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we investigated the role of OsMADS51, a type I MADS-box gene in the short-day (SD) promotion pathway in rice (Oryza sativa)”
e7“These results demonstrate that OsMADS51 functions downstream of OsGI”
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