Abstract
Development of highly efficient and reproducible plant regeneration system has tremendous potential to provide improved technology to assist in genetic transformation of indica rice cultivars for their further exploitation in selection. For the development of a highly reproducible regeneration system through somatic embryogenesis, mature embryos of highly popular rice cultivars i.e., Govind (for rainfed areas), Pusa Basmati-1 (aromatic basmati) and Jaya (for irrigated areas) were used. Optimum callus formation (%) to MS medium supplemented with 2, 4-D was obtained at 12.0 microM in Govind, 14.0 microM in Jaya and 15.0 microM in Pusa Basmati-1. All the cultivars showed good proliferation on MS medium without hormone. In Govind, highest embryogenic response was observed in MS medium supplemented with 2, 4-D (0.4 microM) + kinetin (0.4 microM), while in Pusa Basmati-1 with 2, 4-D (0.4 microM) + kinetin (2.0 microM) and in Jaya on hormone-free MS medium. Excellent embryo regeneration in Govind was observed on MS medium supplemented with low concentrations (1.1 microM) of BAP or hormone-free MS medium, while in Pusa Basmati-1 and Jaya embryogenesis was observed on MS medium supplemented with higher concentration of BAP (2.2 microM). Similarly, maximum plantlets with proliferated roots were observed in Govind on hormone-free MS medium, while in Pusa Basmati-1 and Jaya on MS medium supplemented with high concentration of NAA (4.0 microM). Developed plantlets were further successfully acclimatized and grown under pot culture up to maturity. Further the yield potential of in vitro developed plants was accessed at par to the direct seeded one under pot culture. Present, protocol standardizes somatic embryogenesis and efficient regeneration of agronomically important, high yielding and diverse indica rice cultivars which can be utilized as an efficient tool for molecular studies and genetic transformation in future.
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Claims 16
Claim · model-written summary
Optimum callus formation for Govind occurred at 12.0 microM 2, 4-D.
- Taxon
- rice
Oryza sativae0t0 - Material
- Govinde0
- Treatment
- MS medium supplemented with 2, 4-De0
- Outcome
- Optimum callus formation (%)e0
- Direction
- Optimume0
- Value
- 12.0 microMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimum callus formation (%) to MS medium supplemented with 2, 4-D was obtained at 12.0 microM in Govind”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Optimum callus formation for Jaya occurred at 14.0 microM 2, 4-D.
- Taxon
- riceOryza sativat0
- Material
- Jayae0
- Treatment
- MS medium supplemented with 2, 4-De1
- Outcome
- Optimum callus formation (%)e1
- Direction
- Optimume1
- Value
- 14.0 microMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“14.0 microM in Jaya”
e1“Optimum callus formation (%) to MS medium supplemented with 2, 4-D”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Optimum callus formation for Pusa Basmati-1 occurred at 15.0 microM 2, 4-D.
- Taxon
- riceOryza sativat0
- Material
- Pusa Basmati-1e0
- Treatment
- MS medium supplemented with 2, 4-De1
- Outcome
- Optimum callus formation (%)e1
- Direction
- Optimume1
- Value
- 15.0 microMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“15.0 microM in Pusa Basmati-1”
e1“Optimum callus formation (%) to MS medium supplemented with 2, 4-D”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
All cultivars proliferated well on hormone-free MS medium.
- Taxon
- riceOryza sativat0
- Treatment
- MS medium without hormonee0
- Outcome
- good proliferatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the cultivars showed good proliferation on MS medium without hormone”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Govind had its highest embryogenic response with 0.4 microM 2, 4-D and 0.4 microM kinetin.
- Taxon
- riceOryza sativat0
- Material
- Govinde0
- Treatment
- MS medium supplemented with 2, 4-D (0.4 microM) + kinetin (0.4 microM)e0
- Outcome
- embryogenic responsee0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Govind, highest embryogenic response was observed in MS medium supplemented with 2, 4-D (0.4 microM) + kinetin (0.4 microM)”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Pusa Basmati-1 had its highest embryogenic response with 0.4 microM 2, 4-D and 2.0 microM kinetin.
- Taxon
- riceOryza sativat0
- Material
- Pusa Basmati-1e0
- Treatment
- 2, 4-D (0.4 microM) + kinetin (2.0 microM)e0
- Outcome
- embryogenic responsee1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while in Pusa Basmati-1 with 2, 4-D (0.4 microM) + kinetin (2.0 microM)”
e1“highest embryogenic response”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Jaya had its highest embryogenic response on hormone-free MS medium.
- Taxon
- riceOryza sativat0
- Material
- Jayae0
- Treatment
- hormone-free MS mediume0
- Outcome
- embryogenic responsee1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Jaya on hormone-free MS medium”
e1“highest embryogenic response”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Govind showed excellent embryo regeneration with 1.1 microM BAP.
- Taxon
- riceOryza sativat0
- Material
- Govinde0
- Treatment
- MS medium supplemented with low concentrations (1.1 microM) of BAPe0
- Outcome
- embryo regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Excellent embryo regeneration in Govind was observed on MS medium supplemented with low concentrations (1.1 microM) of BAP”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Govind showed excellent embryo regeneration on hormone-free MS medium.
- Taxon
- riceOryza sativat0
- Material
- Govinde0
- Treatment
- hormone-free MS mediume0
- Outcome
- embryo regeneratione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Excellent embryo regeneration in Govind was observed on MS medium supplemented with low concentrations (1.1 microM) of BAP or hormone-free MS medium”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Pusa Basmati-1 underwent embryogenesis with 2.2 microM BAP.
- Taxon
- riceOryza sativat0
- Material
- Pusa Basmati-1e0
- Treatment
- MS medium supplemented with higher concentration of BAP (2.2 microM)e0
- Outcome
- embryogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in Pusa Basmati-1 and Jaya embryogenesis was observed on MS medium supplemented with higher concentration of BAP (2.2 microM)”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Jaya underwent embryogenesis with 2.2 microM BAP.
- Taxon
- riceOryza sativat0
- Material
- Jayae0
- Treatment
- MS medium supplemented with higher concentration of BAP (2.2 microM)e0
- Outcome
- embryogenesise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pusa Basmati-1 and Jaya embryogenesis was observed on MS medium supplemented with higher concentration of BAP (2.2 microM)”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Govind produced the most plantlets with proliferated roots on hormone-free MS medium.
- Taxon
- riceOryza sativat0
- Material
- Govinde0
- Treatment
- hormone-free MS mediume0
- Outcome
- plantlets with proliferated rootse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maximum plantlets with proliferated roots were observed in Govind on hormone-free MS medium”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Pusa Basmati-1 produced the most plantlets with proliferated roots with 4.0 microM NAA.
- Taxon
- riceOryza sativat0
- Material
- Pusa Basmati-1e0
- Treatment
- MS medium supplemented with high concentration of NAA (4.0 microM)e0
- Outcome
- plantlets with proliferated rootse1
- Direction
- maximume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“while in Pusa Basmati-1 and Jaya on MS medium supplemented with high concentration of NAA (4.0 microM)”
e1“maximum plantlets with proliferated roots”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Jaya produced the most plantlets with proliferated roots with 4.0 microM NAA.
- Taxon
- riceOryza sativat0
- Material
- Jayae0
- Treatment
- MS medium supplemented with high concentration of NAA (4.0 microM)e0
- Outcome
- plantlets with proliferated rootse1
- Direction
- maximume1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pusa Basmati-1 and Jaya on MS medium supplemented with high concentration of NAA (4.0 microM)”
e1“maximum plantlets with proliferated roots”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Regenerated plantlets acclimatized and grew to maturity in pots.
- Taxon
- riceOryza sativat0
- Outcome
- successfully acclimatized and grown under pot culture up to maturitye0
- Setting
- pot culturee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Developed plantlets were further successfully acclimatized and grown under pot culture up to maturity”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Yield potential of in vitro plants was at par with direct-seeded plants in pots.
- Taxon
- riceOryza sativat0
- Treatment
- in vitro developed plantse0
- Outcome
- yield potentiale0
- Direction
- at pare0
- Comparator
- direct seeded onee0
- Setting
- pot culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“yield potential of in vitro developed plants was accessed at par to the direct seeded one under pot culture”
t0“rice (Oryza sativa L.)”
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