Abstract
AIMS: To explore biocontrol potential of 39 DAEB isolates (doubly antagonistic towards both Verticillium dahliae Kleb and Fusarium oxysporum) against verticillium wilt of cotton and to elucidate colonization and category characteristics of an endophytic bacterium with significant biocontrol activity. METHODS AND RESULTS: Thirty-nine antagonistic endophytic bacteria strains were tested for their ability to control verticillium wilt in cotton plants caused by a defoliating pathotype of V. dahliae 107 in cotton under controlled conditions. The biocontrol trial revealed that an endophytic bacterium, designated HA02, showed a significant biocontrol activity to V. dahliae 107. After cotton seedlings were inoculated with a gfp gene-tagged HA02 (HA02-gfp), HA02-gfp populations were higher in the root than in the stem; in addition, the HA02-gfp was distributed in the maturation zone of cotton root. Furthermore, HA02-gfp also colonized seedlings of maize, rape and soybean after the bacteria inoculation. Phylogenetic trees based on 16S rDNA sequences combined with morphological, physiological and identification showed that the bacterium belongs to the Enterobacter genus. CONCLUSIONS: Our results showed that only 1 of 39 DAEB isolates demonstrated more efficient biocontrol potential towards V. dahliae 107 in greenhouse and field trials. HA02-gfp mainly colonized cotton in roots. In addition, we quantitatively observed HA02 colonization in other hosts. HA02 belongs to the Enterobacter genus. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study on biocontrol to defoliating pathotype of V. dahliae Kleb by endophytic bacteria. The HA02 showed effective biocontrol to V. dahliae 107 in greenhouse and field trials. Furthermore, we assessed the quantitative and qualitative colonization of HA02 in cotton seedlings. Our study provides basic information to further explore managing strategies to control this critical disease.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
HA02 significantly controlled cotton wilt caused by V. dahliae 107.
- Taxon
- cotton plants
Gossypium hirsutume1 - Treatment
- HA02e0
- Interactor
- V. dahliae 107
Verticillium dahliaee0pathogen - Second interactor
- HA02e0biocontrol_agent
- Outcome
- V. dahliae 107e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an endophytic bacterium, designated HA02, showed a significant biocontrol activity to V. dahliae 107”
e1“verticillium wilt in cotton plants caused by a defoliating pathotype of V. dahliae 107”
Claim · model-written summary
HA02-gfp populations were higher in cotton roots than stems.
- Taxon
- cottonGossypium hirsutume1
- Organ / stage
- roote0
- Interactor
- HA02-gfpe0biocontrol_agent
- Outcome
- HA02-gfp populationse0
- Direction
- highere0
- Comparator
- steme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HA02-gfp populations were higher in the root than in the stem”
e1“maturation zone of cotton root”
Claim · model-written summary
HA02-gfp was distributed in the maturation zone of cotton root.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- roote0
- Interactor
- HA02-gfpe0biocontrol_agent
- Outcome
- HA02-gfp was distributed in the maturation zonee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HA02-gfp was distributed in the maturation zone of cotton root”
Claim · model-written summary
HA02-gfp colonized maize seedlings following inoculation.
- Treatment
- bacteria inoculatione0
- Interactor
- HA02-gfpe0biocontrol_agent
- Outcome
- colonized seedlings of maizee0
Zea maysNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HA02-gfp also colonized seedlings of maize, rape and soybean after the bacteria inoculation”
Claim · model-written summary
HA02-gfp colonized rape seedlings following inoculation.
- Taxon
- rape
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse0 - Treatment
- bacteria inoculatione0
- Interactor
- HA02-gfpe0biocontrol_agent
- Outcome
- rapee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“HA02-gfp also colonized seedlings of maize, rape and soybean after the bacteria inoculation”
Claim · model-written summary
HA02-gfp colonized soybean seedlings following inoculation.
- Taxon
- soybean
Glycine maxe0 - Treatment
- bacteria inoculatione0
- Interactor
- HA02-gfpe0biocontrol_agent
- Outcome
- soybeane0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HA02-gfp also colonized seedlings of maize, rape and soybean after the bacteria inoculation”
Claim · model-written summary
The HA02 bacterium belongs to the Enterobacter genus.
- Taxon
- cottonGossypium hirsutume1
- Interactor
- Enterobacter
Enterobactere0biocontrol_agent - Outcome
- belongs to the Enterobacter genuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the bacterium belongs to the Enterobacter genus”
e1“HA02-gfp was distributed in the maturation zone of cotton root”
Claim · model-written summary
Only one of 39 isolates showed more efficient control of cotton V. dahliae 107 in greenhouse and field trials.
- Taxon
- cotton plantsGossypium hirsutumt0
- Interactor
- V. dahliae 107Verticillium dahliaee0pathogen
- Outcome
- biocontrol potential towards V. dahliae 107e0
- Value
- 1 of 39e0
- Setting
- greenhouse and field trialse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only 1 of 39 DAEB isolates demonstrated more efficient biocontrol potential towards V. dahliae 107 in greenhouse and field trials”
t0“cotton plants”
The same questions in other papers
inoculation · organism · Brassica napus (oilseed rape)Positive and negative results
7 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
5 papers more in this group
See all 9 papers →inoculation · organism · Glycine max (soybean)Positive and negative results
23 papers in the same direction as this paper · 8 in the opposite direction · 1 reporting no difference from an alternative
- no effect
- no effect
- negative
29 papers more in this group
See all 33 papers →inoculation · organism · Zea mays (maize)Positive and negative results
33 papers in the same direction as this paper · 6 in the opposite direction
- no effect
- no effect
- no effect
36 papers more in this group
See all 40 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.