Paper · 2010 · AFRICAN JOURNAL OF BIOTECHNOLOGY

In vitro storage of synthetic seeds: Effect of different storage conditions and intervals on their conversion ability

Muhammad Ikhlaq; Ishfaq Ahmad Hafiz; Maurizio Micheli; et al.

6
claims
12
verbatim quotes
40
citations
3
research areas
✓
Open access

Abstract

In vitro derived shoots of olive cv. Moraiolo were employed in synthetic seeds preparation by alginate encapsulation, and then stored in artificial endosperm solution at cold (4°C) and room storage (21 ± 2°C) conditions in interaction with different storage intervals of 0, 15, 30, 45 and 60 days to evaluate the comparative regrowth and conversion capacity of synthetic seeds. Cold stored synthetic seeds were superior in terms of their regrowth capacity than that of room stored ones for all the growth parameters studied. A promising degree of interaction was observed between 4°C and 45 days of storage interval for regrowth percentage as well as for shoot and root development. Moreover, an ascending trend was recorded in conversion potential with an increase in storage intervals up to 45 days (S3) whereas there was a declining trend after that up to 60 days (S4). Moreover plantlets regenerated from synthetic seeds, with 4 - 6 fully expanded leaves and well developed root system were successfully acclimatized under ex vitroconditions. The protocol can be used for germplasm exchange of woody trees and preparation of synthetic seed. Key word: Synthetic, seed, olive, encapsulation, storage, conversion.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cold stored synthetic seeds had superior regrowth to room stored seeds.

Taxon
oliveOlea europaeat0
Treatment
Cold storede0
Outcome
regrowth capacitye0
Direction
superiore0
Comparator
room stored onese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Cold stored synthetic seeds were superior in terms of their regrowth capacity than that of room stored ones for all the growth parameters studied.”

✓ located verbatim in the source · abstract, characters 378–524 · show in text ↑
t0

“olive cv. Moraiolo”

✓ located verbatim in the source · abstract, characters 27–45 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Storage at 4°C for 45 days showed promising regrowth, shoot and root development.

Taxon
oliveOlea europaeat0
Treatment
4°C and 45 days of storage intervale0
Outcome
regrowth percentage as well as for shoot and root developmente0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“a promising degree of interaction was observed between 4°C and 45 days of storage interval for regrowth percentage as well as for shoot and root development”

✓ located after whitespace/quote normalisation · abstract, characters 525–681 · show in text ↑
t0

“olive cv. Moraiolo”

✓ located verbatim in the source · abstract, characters 27–45 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Conversion potential rose with storage intervals through 45 days.

Taxon
oliveOlea europaeat0
Treatment
storage intervals up to 45 days (S3)e0
Outcome
conversion potentiale0
Direction
ascendinge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“an ascending trend was recorded in conversion potential with an increase in storage intervals up to 45 days (S3)”

✓ located verbatim in the source · abstract, characters 693–805 · show in text ↑
t0

“olive cv. Moraiolo”

✓ located verbatim in the source · abstract, characters 27–45 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Conversion potential declined after 45 days through 60 days.

Taxon
oliveOlea europaeat0
Treatment
60 days (S4)e0
Outcome
conversion potentiale0
Direction
declininge0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“conversion potential with an increase in storage intervals up to 45 days (S3) whereas there was a declining trend after that up to 60 days (S4)”

✓ located verbatim in the source · abstract, characters 728–871 · show in text ↑
t0

“olive cv. Moraiolo”

✓ located verbatim in the source · abstract, characters 27–45 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Regenerated plantlets were successfully acclimatized ex vitro.

Taxon
oliveOlea europaeat0
Organ / stage
plantletse0
Outcome
successfully acclimatizede0
Value
4 - 6 fully expanded leavese0
Setting
ex vitroconditionse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“plantlets regenerated from synthetic seeds, with 4 - 6 fully expanded leaves and well developed root system were successfully acclimatized under ex vitroconditions.”

✓ located verbatim in the source · abstract, characters 882–1046 · show in text ↑
t0

“olive cv. Moraiolo”

✓ located verbatim in the source · abstract, characters 27–45 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The protocol was proposed for germplasm exchange and synthetic seed preparation.

Taxon
oliveOlea europaeat0
Outcome
germplasm exchange of woody trees and preparation of synthetic seede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Olea europaea
Olea europaea · TaxonHans Hillewaert · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The protocol can be used for germplasm exchange of woody trees and preparation of synthetic seed.”

✓ located verbatim in the source · abstract, characters 1047–1144 · show in text ↑
t0

“olive cv. Moraiolo”

✓ located verbatim in the source · abstract, characters 27–45 · show in text ↑

storage · growth · Olea europaea (common olive)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

cold · growth · Olea europaea (common olive)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.