Abstract
Soybean plants and Rhizobium japonicum 122 DES, a hydrogen uptake-positive strain, were cultured in media purified to remove Ni. Supplemental Ni had no significant effect on the dry matter or total N content of plants. However, the addition of Ni to both nitrate-grown and symbiotically grown plants resulted in a 7- to 10-fold increase in urease activity (urea amidohydrolase, EC 3.5.1.5) in leaves and significantly increased the hydrogenase activity (EC 1.18.3.1) in isolated nodule bacteroids. When cultured under chemolithotrophic conditions, free-living R. japonicum required Ni for growth and for the expression of hydrogenase activity. Hydrogenase activity was minimal or not detectable in cells incubated either without Ni or with Ni and chloramphenicol. Ni is required for derepression of hydrogenase activity and apparently protein synthesis is necessary for the participation of Ni in hydrogenase expression. The addition of Cr, V, Sn, and Pb in place of Ni failed to stimulate the activity of hydrogenase in R. japonicum and urease in soybean leaves. The evidence indicates that Ni is an important micronutrient element in the biology of the soybean plant and R. japonicum.
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Claims 9
Claim · model-written summary
Supplemental Ni has no significant effect on dry matter or total N content of soybean plants.
- Taxon
- soybean
Glycine maxt0 - Treatment
- Supplemental Nie1
- Outcome
- dry matter or total N content of plantse1
- Direction
- no significant effecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“Supplemental Ni had no significant effect on the dry matter or total N content of plants”
Claim · model-written summary
Adding Ni to nitrate-grown and symbiotically grown soybean plants increases urease activity 7- to 10-fold in leaves.
- Taxon
- soybeanGlycine maxt0
- Material
- nitrate-grown and symbiotically grown plantse1
- Organ / stage
- leavese1
- Treatment
- addition of Nie1
- Outcome
- urease activitye1
- Direction
- increasee1
- Value
- 7- to 10-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“However, the addition of Ni to both nitrate-grown and symbiotically grown plants resulted in a 7- to 10-fold increase in urease activity (urea amidohydrolase, EC 3.5.1.5) in leaves and significantly increased the hydrogenase activity (EC 1.18.3.1) in isolated nodule bacteroids”
Claim · model-written summary
Adding Ni significantly increases hydrogenase activity in isolated nodule bacteroids of Rhizobium japonicum.
- Taxon
- soybeanGlycine maxt0
- Organ / stage
- isolated nodule bacteroidse1
- Treatment
- addition of Nie1
- Interactor
- Rhizobium japonicumt1symbiont
- Outcome
- hydrogenase activitye1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
t1“hydrogen-dependent growth of Rhizobium japonicum”
e1“However, the addition of Ni to both nitrate-grown and symbiotically grown plants resulted in a 7- to 10-fold increase in urease activity (urea amidohydrolase, EC 3.5.1.5) in leaves and significantly increased the hydrogenase activity (EC 1.18.3.1) in isolated nodule bacteroids”
Claim · model-written summary
Free-living R. japonicum requires Ni for growth under chemolithotrophic conditions.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Nie1
- Interactor
- R. japonicume1symbiont
- Outcome
- growthe1
- Qualifier
- When cultured under chemolithotrophic conditionse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“When cultured under chemolithotrophic conditions, free-living R. japonicum required Ni for growth and for the expression of hydrogenase activity”
Claim · model-written summary
Free-living R. japonicum requires Ni for expression of hydrogenase activity under chemolithotrophic conditions.
- Taxon
- soybeanGlycine maxt0
- Treatment
- Nie1
- Interactor
- R. japonicume1symbiont
- Outcome
- expression of hydrogenase activitye1
- Qualifier
- When cultured under chemolithotrophic conditionse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“When cultured under chemolithotrophic conditions, free-living R. japonicum required Ni for growth and for the expression of hydrogenase activity”
Claim · model-written summary
Hydrogenase activity is minimal or undetectable in R. japonicum cells without Ni or with Ni and chloramphenicol.
- Taxon
- soybeanGlycine maxt0
- Treatment
- without Nie1
- Interactor
- R. japonicume2symbiont
- Outcome
- Hydrogenase activitye1
- Direction
- minimal or not detectablee1
- Comparator
- with Nie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“Hydrogenase activity was minimal or not detectable in cells incubated either without Ni or with Ni and chloramphenicol”
e2“When cultured under chemolithotrophic conditions, free-living R. japonicum required Ni for growth and for the expression of hydrogenase activity”
Claim · model-written summary
Ni is required for derepression of hydrogenase activity, and protein synthesis is necessary for Ni to act in hydrogenase expression.
- Taxon
- soybeanGlycine maxt0
- Compound
- Nie1
- Outcome
- derepression of hydrogenase activitye1
- Qualifier
- protein synthesis is necessarye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“Ni is required for derepression of hydrogenase activity and apparently protein synthesis is necessary for the participation of Ni in hydrogenase expression”
Claim · model-written summary
Adding Cr, V, Sn or Pb in place of Ni fails to stimulate hydrogenase activity in R. japonicum.
- Taxon
- soybeanGlycine maxt0
- Treatment
- addition of Cr, V, Sn, and Pbe1
- Interactor
- R. japonicume1symbiont
- Outcome
- activity of hydrogenasee1
- Direction
- failed to stimulatee1
- Comparator
- Nie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“The addition of Cr, V, Sn, and Pb in place of Ni failed to stimulate the activity of hydrogenase in R. japonicum and urease in soybean leaves”
Claim · model-written summary
Adding Cr, V, Sn or Pb in place of Ni fails to stimulate urease activity in soybean leaves.
- Taxon
- soybeanGlycine maxt0
- Treatment
- addition of Cr, V, Sn, and Pbe1
- Outcome
- urease in soybean leavese1
- Direction
- failed to stimulatee1
- Comparator
- Nie1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“urease activity in soybean leaves”
e1“The addition of Cr, V, Sn, and Pb in place of Ni failed to stimulate the activity of hydrogenase in R. japonicum and urease in soybean leaves”
The same questions in other papers
nickel atom · growth · Glycine max (soybean)Results differ
4 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →lead atom · organism · Glycine max (soybean)Positive and negative results
1 reporting no difference from an alternative
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.