Abstract
The effect of single actinobacterial endophyte seed inoculants and a mixed microbial soil inoculant on the indigenous endophytic actinobacterial population in wheat roots was investigated by using the molecular technique terminal restriction fragment length polymorphism (T-RFLP). Wheat was cultivated either from seeds coated with the spores of single pure actinobacterial endophytes of Microbispora sp. strain EN2, Streptomyces sp. strain EN27, and Nocardioides albus EN46 or from untreated seeds sown in soil with and without a commercial mixed microbial soil inoculant. The endophytic actinobacterial population within the roots of 6-week-old wheat plants was assessed by T-RFLP. Colonization of the wheat roots by the inoculated actinobacterial endophytes was detected by T-RFLP, as were 28 to 42 indigenous actinobacterial genera present in the inoculated and uninoculated plants. The presence of the commercial mixed inoculant in the soil reduced the endophytic actinobacterial diversity from 40 genera to 21 genera and reduced the detectable root colonization by approximately half. The results indicate that the addition of a nonadapted microbial inoculum to the soil disrupted the natural actinobacterial endophyte population, reducing diversity and colonization levels. This was in contrast to the addition of a single actinobacterial endophyte to the wheat plant, where the increase in colonization level could be confirmed even though the indigenous endophyte population was not adversely affected.
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Claims 6
Claim · model-written summary
Inoculated actinobacterial endophytes colonized wheat roots.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- actinobacterial endophytese0symbiont
- Outcome
- Colonization of the wheat rootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Colonization of the wheat roots by the inoculated actinobacterial endophytes was detected by T-RFLP”
Claim · model-written summary
Between 28 and 42 indigenous actinobacterial genera were detected in inoculated and uninoculated wheat roots.
- Taxon
- wheatTriticum aestivume1
- Interactor
- indigenous actinobacterial generae0symbiont
- Outcome
- indigenous actinobacterial genera presente0
- Value
- 28 to 42e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“28 to 42 indigenous actinobacterial genera present in the inoculated and uninoculated plants”
e1“in wheat roots was investigated”
Claim · model-written summary
The mixed soil inoculant reduced wheat root endophyte diversity from 40 to 21 genera.
- Taxon
- wheatTriticum aestivume1
- Treatment
- commercial mixed inoculante0
- Interactor
- actinobacteriale0symbiont
- Outcome
- endophytic actinobacterial diversitye0
- Direction
- reducede0
- Value
- 21 generae0
- Qualifier
- 40 generae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of the commercial mixed inoculant in the soil reduced the endophytic actinobacterial diversity from 40 genera to 21 genera”
e1“in wheat roots was investigated”
Claim · model-written summary
The mixed inoculant reduced detectable wheat root colonization by approximately half.
- Taxon
- wheatTriticum aestivume1
- Treatment
- commercial mixed inoculante0
- Interactor
- actinobacteriale0symbiont
- Outcome
- detectable root colonizatione0
- Direction
- reducede0
- Value
- approximately halfe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The presence of the commercial mixed inoculant in the soil reduced the endophytic actinobacterial diversity from 40 genera to 21 genera and reduced the detectable root colonization by approximately half”
e1“in wheat roots was investigated”
Claim · model-written summary
A single actinobacterial endophyte increased wheat root colonization.
- Taxon
- wheatTriticum aestivume0
- Treatment
- addition of a single actinobacterial endophytee0
- Interactor
- actinobacterial endophytee0symbiont
- Outcome
- colonization levele0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the addition of a single actinobacterial endophyte to the wheat plant, where the increase in colonization level could be confirmed”
Claim · model-written summary
Single endophyte addition did not adversely affect the indigenous endophyte population.
- Taxon
- wheatTriticum aestivume1
- Interactor
- endophytee0symbiont
- Outcome
- indigenous endophyte populatione0
- Direction
- not adversely affectede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the indigenous endophyte population was not adversely affected”
e1“to the wheat plant”
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