Abstract
In this report, all of the criteria necessary for the demonstration of nitrogen fixation in wheat (Triticum aestivum L.), the world's most important crop, are shown upon inoculation with a nitrogen-fixing bacterium, Klebsiella pneumoniae 342 (Kp342). Kp342 relieved nitrogen (N) deficiency symptoms and increased total N and N concentration in the plant. Nitrogen fixation was confirmed by 15N isotope dilution in the plant tissue and in a plant product, chlorophyll. All of these observations were in contrast to uninoculated plants, plants inoculated with a nitrogen-fixing mutant of Kp342, and plants inoculated with dead Kp342 cells. Nitrogenase reductase was produced by Kp342 in the intercellular space of the root cortex. Wild-type Kp342 and the nifH mutant colonized the interior of wheat roots in equal numbers on a fresh weight basis. The nitrogen fixation phenotype described here was specific to cv. Trenton. Inoculation of cvs. Russ or Stoa with Kp342 resulted in no relief of nitrogen deficiency symptoms.
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Claims 8
Claim · model-written summary
Kp342 relieved nitrogen deficiency symptoms in wheat.
- Taxon
- Wheat
Triticum aestivumt0 - Treatment
- Kp342e0
- Interactor
- Klebsiella pneumoniae 342Klebsiella variicola 342t0symbiont
- Outcome
- nitrogen (N) deficiency symptomse0
- Direction
- relievede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kp342 relieved nitrogen (N) deficiency symptoms”
t0“Wheat Provided by Klebsiella pneumoniae 342”
Claim · model-written summary
Kp342 increased total plant nitrogen and nitrogen concentration.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Kp342e1
- Interactor
- Klebsiella pneumoniae 342Klebsiella variicola 342t0symbiont
- Outcome
- total N and N concentration in the plante0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased total N and N concentration in the plant”
e1“Kp342 relieved nitrogen”
t0“Wheat Provided by Klebsiella pneumoniae 342”
Claim · model-written summary
Isotope dilution confirmed nitrogen fixation in plant tissue and chlorophyll.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- plant tissuee0
- Interactor
- Klebsiella pneumoniae 342Klebsiella variicola 342t0symbiont
- Outcome
- Nitrogen fixatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrogen fixation was confirmed by 15N isotope dilution in the plant tissue and in a plant product, chlorophyll.”
t0“Wheat Provided by Klebsiella pneumoniae 342”
Claim · model-written summary
Kp342 produced nitrogenase reductase in the root cortex intercellular space.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- root cortexe0
- Interactor
- Kp342e0symbiont
- Gene
- Nitrogenase reductasee0
- Outcome
- Nitrogenase reductase was producede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrogenase reductase was produced by Kp342 in the intercellular space of the root cortex.”
t0“Wheat Provided by Klebsiella pneumoniae 342”
Claim · model-written summary
Wild-type Kp342 and its nifH mutant colonized wheat root interiors in equal numbers.
- Taxon
- wheatTriticum aestivume0
- Interactor
- Kp342e0symbiont
- Gene
- nifHe0
- Outcome
- colonized the interior of wheat roots in equal numberse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wild-type Kp342 and the nifH mutant colonized the interior of wheat roots in equal numbers on a fresh weight basis.”
Claim · model-written summary
The nitrogen fixation phenotype was specific to cultivar Trenton.
- Taxon
- WheatTriticum aestivumt0
- Material
- cv. Trentone0
- Interactor
- Klebsiella pneumoniae 342Klebsiella variicola 342t0symbiont
- Outcome
- nitrogen fixation phenotypee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The nitrogen fixation phenotype described here was specific to cv. Trenton.”
t0“Wheat Provided by Klebsiella pneumoniae 342”
Claim · model-written summary
Kp342 did not relieve nitrogen deficiency symptoms in cultivar Russ.
- Taxon
- WheatTriticum aestivumt0
- Material
- Russe0
- Treatment
- Inoculation of cvs. Russ or Stoa with Kp342e0
- Interactor
- Kp342e0symbiont
- Outcome
- nitrogen deficiency symptomse0
- Direction
- no reliefe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of cvs. Russ or Stoa with Kp342 resulted in no relief of nitrogen deficiency symptoms.”
t0“Wheat Provided by Klebsiella pneumoniae 342”
Claim · model-written summary
Kp342 did not relieve nitrogen deficiency symptoms in cultivar Stoa.
- Taxon
- WheatTriticum aestivumt0
- Material
- Stoae0
- Treatment
- Inoculation of cvs. Russ or Stoa with Kp342e0
- Interactor
- Kp342e0symbiont
- Outcome
- nitrogen deficiency symptomse0
- Direction
- no reliefe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of cvs. Russ or Stoa with Kp342 resulted in no relief of nitrogen deficiency symptoms.”
t0“Wheat Provided by Klebsiella pneumoniae 342”
The same questions in other papers
inoculation · disease · Triticum aestivum (wheat)Positive and negative results
59 papers in the same direction as this paper · 104 in the opposite direction
- positive
- positive
- positive
160 papers more in this group
See all 164 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.